Restriction map for
pF1KB6404
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsiYI BslI BsmBI BsmI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I Cfr13I CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 219 CGCG CG^CG
AlwNI 610 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 602 CCTCAGG CC^T[ACGT]AGG
BanII 348 GAGCTC G[GA]GC[CT]^C
BfaI 961 CTAG C^TAG
BsaJI 29 CCGAGG C^C[ACGT][ACGT]GG
BseRI 10 GAGGAG GAGGAG(10/8)
BsiEI 524 CGGCCG CG[GA][CT]^CG
Bsp1407I 879 TGTACA T^GTACA
BspDI 628 ATCGAT AT^CGAT
BsrGI 879 TGTACA T^GTACA
BstUI 219 CGCG CG^CG
Bsu36I 602 CCTCAGG CC^T[ACGT]AGG
ClaI 628 ATCGAT AT^CGAT
EagI 524 CGGCCG C^GGCCG
EarI 990 GAAGAG CTCTTC(1/4)
EclXI 524 CGGCCG C^GGCCG
Eco52I 524 CGGCCG C^GGCCG
Eco81I 602 CCTCAGG CC^T[ACGT]AGG
HgaI 621 GACGC GACGC(5/10)
HinfI 292 GATTC G^A[ACGT]TC
Ksp632I 990 GAAGAG CTCTTC(1/4)
MaeI 961 CTAG C^TAG
MvnI 219 CGCG CG^CG
SacI 348 GAGCTC GAGCT^C
SfaNI 587 GCATC GCATC(5/9)
SfcI 726 CTACAG C^T[GA][CT]AG
SmlI 490 CTCAAG C^T[CT][GA]AG
SspBI 879 TGTACA T^GTACA
TaqI 629 TCGA T^CGA
TfiI 292 GATTC G^A[AT]TC
TthHB8I 629 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 753 GTAC GT^AC
880 GTAC
AspHI 41 GAGCAC G[AT]GC[AT]^C
348 GAGCTC
AvaI 22 CCCGAG C^[CT]CG[GA]G
351 CTCGGG
BalI 126 TGGCCA TGG^CCA
759 TGGCCA
BbsI 618 GAAGAC GAAGAC(2/6)
747 GAAGAC
BbvI 401 GCTGC GCAGC(8/12)
955 GCTGC
BmyI 41 GAGCAC G[AGT]GC[ACT]^C
348 GAGCTC
BpuAI 618 GAAGAC GAAGAC(2/6)
747 GAAGAC
BsaI 25 GAGACC GGTCTC(1/5)
577 GGTCTC
BsiHKAI 41 GAGCAC G[AT]GC[AT]^C
348 GAGCTC
BsoBI 22 CCCGAG C^[CT]CG[GA]G
351 CTCGGG
Bsp1286I 41 GAGCAC G[AGT]GC[ACT]^C
348 GAGCTC
BssKI 281 CCTGG ^CC[ACGT]GG
687 CCTGG
BstNI 281 CCTGG CC^[AT]GG
687 CCTGG
CfoI 218 GCGC GCG^C
958 GCGC
EcoRII 281 CCTGG ^CC[AT]GG
687 CCTGG
HhaI 218 GCGC GCG^C
958 GCGC
HinP1I 218 GCGC G^CGC
958 GCGC
HphI 116 TCACC GGTGA(8/7)
927 TCACC
MaeII 344 ACGT A^CGT
751 ACGT
MluNI 126 TGGCCA TGG^CCA
759 TGGCCA
MscI 126 TGGCCA TGG^CCA
759 TGGCCA
MslI 763 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
898 CACACAGATG
MvaI 281 CCTGG CC^[AT]GG
687 CCTGG
MwoI 34 GCCGCCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
949 GCCTCTGCTGC
RsaI 753 GTAC GT^AC
880 GTAC
ScrFI 281 CCTGG CC^[ACGT]GG
687 CCTGG
TseI 401 GCTGC G^C[AT]GC
955 GCTGC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BamHI BsmAI BsmFI BsrI BstF5I
BstYI Cac8I DdeI DpnII EaeI
Fnu4HI FokI HaeIII ItaI MaeIII
MboI MboII MflI MnlI MseI
NdeII NlaIV Sau3AI Tru9I Tsp45I
Tsp509I TspRI XhoII