Restriction map for
pF1KE0028
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# NO CUT ENZYMES ( 95 enzymes) #
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Acc65I AclI AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
BbrPI BbsI BcgI BclI BfrI
BglII BpuAI BsaAI BsaBI BsaI
BsiEI BsiWI BspDI BspHI BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 912 GACGTC GACGT^C
AccIII 916 TCCGGA T^CCGGA
AflIII 2047 ACATGT A^C[GA][CT]GT
AhdI 907 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1153 CCTGAGG CC^T[ACGT]AGG
Asp700I 111 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 907 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2118 CCTAGG C^CTAGG
BamHI 1674 GGATCC G^GATCC
BbeI 992 GGCGCC GGCGC^C
BbvCI 942 CCTCAGC CCTCAGC(-5/-2)
BfaI 2119 CTAG C^TAG
BglI 2117 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2118 CCTAGG C^CTAGG
BlpI 1087 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1087 GCTGAGC GC^T[ACGT]AGC
BseAI 916 TCCGGA T^CCGGA
BsmBI 1426 CGTCTC CGTCTC(1/5)
BsmI 747 GAATGC GAATGC(1/-1)
BspEI 916 TCCGGA T^CCGGA
BspLU11I 2047 ACATGT A^CATGT
BsrFI 983 ACCGGC [GA]^CCGG[CT]
BssSI 770 CTCGTG CACGAG(-5/-1)
BstEII 2142 GGTCACC G^GT[ACGT]ACC
BstPI 2142 GGTCACC G^GT[ACGT]ACC
BstYI 1674 GGATCC [GA]^GATC[CT]
Bsu36I 1153 CCTGAGG CC^T[ACGT]AGG
CelII 1087 GCTGAGC GC^T[ACGT]AGC
Cfr10I 983 ACCGGC [GA]^CCGG[CT]
DraIII 767 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 25 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 907 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1153 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2142 GGTCACC G^GT[ACGT]ACC
HaeII 992 GGCGCC [GA]GCGC^[CT]
HindIII 592 AAGCTT A^AGCTT
KasI 992 GGCGCC G^GCGCC
KspI 54 CCGCGG CCGC^GG
MaeI 2119 CTAG C^TAG
MflI 1674 GGATCC [GA]^GATC[CT]
MroI 916 TCCGGA T^CCGGA
MseI 154 TTAA T^TAA
NarI 992 GGCGCC GG^CGCC
SacII 54 CCGCGG CCGC^GG
SbfI 996 CCTGCAGG CCTGCA^GG
SexAI 557 ACCAGGT A^CC[AT]GGT
SfoI 992 GGCGCC GGC^GCC
SphI 506 GCATGC GCATG^C
Sse8387I 996 CCTGCAGG CCTGCA^GG
StuI 659 AGGCCT AGG^CCT
Tru9I 154 TTAA T^TAA
XhoII 1674 GGATCC [GA]^GATC[CT]
XmnI 111 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 289 GTCTAC GT^[AC][GT]AC
1819 GTCTAC
AccII 55 CGCG CG^CG
1788 CGCG
AvaI 1133 CTCGGG C^[CT]CG[GA]G
1174 CCCGAG
BalI 953 TGGCCA TGG^CCA
2124 TGGCCA
BanI 892 GGCACC G^G[CT][GA]CC
992 GGCGCC
BciVI 1969 GGATAC GTATCC(6/5)
1918 GTATCC
BsaWI 916 TCCGGA [AT]^CCGG[AT]
967 TCCGGT
BsoBI 1133 CTCGGG C^[CT]CG[GA]G
1174 CCCGAG
Bsp1407I 1699 TGTACA T^GTACA
1856 TGTACA
BsrDI 602 CATTGC GCAATG(2/0)
1686 CATTGC
BsrGI 1699 TGTACA T^GTACA
1856 TGTACA
BstAPI 66 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
150 GCAGTTAATGC
BstUI 55 CGCG CG^CG
1788 CGCG
BstXI 1899 CCACAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2219 CCACCCTCCTGG
FauI 56 GCGGG CCCGC(4/6)
947 GCGGG
MaeII 913 ACGT A^CGT
1280 ACGT
MluNI 953 TGGCCA TGG^CCA
2124 TGGCCA
MscI 953 TGGCCA TGG^CCA
2124 TGGCCA
MvnI 55 CGCG CG^CG
1788 CGCG
PpuMI 905 AGGACCC [GA]G^G[AT]CC[CT]
2176 GGGACCT
SacI 858 GAGCTC GAGCT^C
1102 GAGCTC
SspBI 1699 TGTACA T^GTACA
1856 TGTACA
TaqI 276 TCGA T^CGA
444 TCGA
TfiI 1452 GAATC G^A[AT]TC
1490 GAATC
Tsp509I 677 AATT ^AATT
2035 AATT
TthHB8I 276 TCGA T^CGA
444 TCGA
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I TspRI