Restriction map for
pF1KB7307
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI ApaI AscI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcnI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaII KasI KspI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2895 GGTACC G^GTACC
AccI 1915 GTAGAC GT^[AC][GT]AC
AciI 36 GCGG CCGC(-3/-1)
AflII 669 CTTAAG C^TTAAG
AflIII 1738 ACATGT A^C[GA][CT]GT
AhdI 3107 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4792 GTGCAC G^TGCAC
AocI 4520 CCTTAGG CC^T[ACGT]AGG
ApaLI 4792 GTGCAC G^TGCAC
Asp718I 2895 GGTACC G^GTACC
AspEI 3107 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 596 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 269 CTCGAG C^[CT]CG[GA]G
BcgI 2040 GCAAAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1359 TGATCA T^GATCA
BfrI 669 CTTAAG C^TTAAG
BglI 3362 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2840 GAGACC GGTCTC(1/5)
BsmBI 4676 CGTCTC CGTCTC(1/5)
BsoBI 269 CTCGAG C^[CT]CG[GA]G
BspLU11I 1738 ACATGT A^CATGT
BsrBI 34 GAGCGG CCGCTC(-3/-3)
BstAPI 4492 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 4520 CCTTAGG CC^T[ACGT]AGG
Eam1105I 3107 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4520 CCTTAGG CC^T[ACGT]AGG
EcoRI 3694 GAATTC G^AATTC
EcoT22I 4643 ATGCAT ATGCA^T
FbaI 1359 TGATCA T^GATCA
HindIII 2239 AAGCTT A^AGCTT
HpaI 3453 GTTAAC GTT^AAC
KpnI 2895 GGTACC GGTAC^C
MaeII 1334 ACGT A^CGT
NdeI 938 CATATG CA^TATG
NsiI 4643 ATGCAT ATGCA^T
PaeR7I 269 CTCGAG C^TCGAG
PflFI 596 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4030 GAAGAGC GCTCTTC(1/4)
SexAI 3471 ACCTGGT A^CC[AT]GGT
SphI 1582 GCATGC GCATG^C
SwaI 810 ATTTAAAT ATTT^AAAT
Tsp45I 4041 GTCAC ^GT[GC]AC
Tth111I 596 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2445 TCTAGA T^CTAGA
XhoI 269 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 904 ATTAAT AT^TAAT
973 ATTAAT
AsnI 904 ATTAAT AT^TAAT
973 ATTAAT
BbsI 325 GAAGAC GAAGAC(2/6)
1609 GAAGAC
BpuAI 325 GAAGAC GAAGAC(2/6)
1609 GAAGAC
BsaBI 577 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTTTGATC
BseRI 266 CTCCTC GAGGAG(10/8)
2890 GAGGAG
BsmI 4361 GAATGC GAATGC(1/-1)
2669 GCATTC
Bsp1407I 1876 TGTACA T^GTACA
3582 TGTACA
BsrGI 1876 TGTACA T^GTACA
3582 TGTACA
BstXI 285 CCAGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4760 CCATGCCCATGG
MamI 577 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTTTGATC
MspA1I 3578 CAGCTG C[AC]G^C[GT]G
4571 CAGCTG
PshBI 904 ATTAAT AT^TAAT
973 ATTAAT
PvuII 3578 CAGCTG CAG^CTG
4571 CAGCTG
SacI 2978 GAGCTC GAGCT^C
4944 GAGCTC
ScaI 2966 AGTACT AGT^ACT
3118 AGTACT
SspBI 1876 TGTACA T^GTACA
3582 TGTACA
SspI 1628 AATATT AAT^ATT
2717 AATATT
XcmI 510 CCATGAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4609 CCACCTACTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbvI BciVI BfaI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI