Restriction map for
pF1KB7868
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssSI BstBI CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 892 GTATAC GT^[AC][GT]AC
AccII 566 CGCG CG^CG
AccIII 842 TCCGGA T^CCGGA
AcyI 307 GGCGCC G[GA]^CG[CT]C
Alw44I 426 GTGCAC G^TGCAC
AlwI 1275 GATCC GGATC(4/5)
ApaLI 426 GTGCAC G^TGCAC
Asp700I 1473 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1323 TGGCCA TGG^CCA
BbeI 307 GGCGCC GGCGC^C
BbrPI 1507 CACGTG CAC^GTG
BcgI 1803 CGAAGCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 249 TGATCA T^GATCA
BsaHI 307 GGCGCC G[GA]^CG[CT]C
BsaWI 842 TCCGGA [AT]^CCGG[AT]
BseAI 842 TCCGGA T^CCGGA
BsgI 187 GTGCAG GTGCAG(16/14)
BspEI 842 TCCGGA T^CCGGA
BspLU11I 96 ACATGT A^CATGT
BsrBI 1243 CCGCTC CCGCTC(-3/-3)
BssHII 565 GCGCGC G^CGCGC
Bst1107I 892 GTATAC GTA^TAC
BstEII 114 GGTGACC G^GT[ACGT]ACC
BstPI 114 GGTGACC G^GT[ACGT]ACC
BstUI 566 CGCG CG^CG
BstXI 290 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 892 GTATAC GTA^TAC
EagI 1531 CGGCCG C^GGCCG
EclXI 1531 CGGCCG C^GGCCG
Eco52I 1531 CGGCCG C^GGCCG
EcoNI 1174 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 114 GGTGACC G^GT[ACGT]ACC
FbaI 249 TGATCA T^GATCA
Hin1I 307 GGCGCC G[GA]^CG[CT]C
HphI 114 GGTGA GGTGA(8/7)
KasI 307 GGCGCC G^GCGCC
MluNI 1323 TGGCCA TGG^CCA
MroI 842 TCCGGA T^CCGGA
MscI 1323 TGGCCA TGG^CCA
MslI 1374 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 566 CGCG CG^CG
NaeI 1694 GCCGGC GCC^GGC
NarI 307 GGCGCC GG^CGCC
NcoI 554 CCATGG C^CATGG
NdeI 1157 CATATG CA^TATG
NgoMIV 1694 GCCGGC G^CCGGC
PflFI 367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1507 CACGTG CAC^GTG
PmlI 1507 CACGTG CAC^GTG
PvuII 83 CAGCTG CAG^CTG
SfaNI 869 GCATC GCATC(5/9)
SfoI 307 GGCGCC GGC^GCC
SmlI 1612 CTCAAG C^T[CT][GA]AG
SphI 1493 GCATGC GCATG^C
StuI 1475 AGGCCT AGG^CCT
TfiI 597 GAATC G^A[AT]TC
Tsp509I 913 AATT ^AATT
Tth111I 367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1473 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 96 ACATGT A^C[GA][CT]GT
1836 ACGTGT
AocI 109 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
BsaAI 895 TACGTG [CT]AC^GT[GA]
1507 CACGTG
BsaI 846 GAGACC GGTCTC(1/5)
1609 GGTCTC
BsiEI 1240 CGACCG CG[GA][CT]^CG
1531 CGGCCG
BspMI 1119 GCAGGT ACCTGC(4/8)
1180 GCAGGT
BsrFI 1502 ACCGGC [GA]^CCGG[CT]
1694 GCCGGC
BstAPI 147 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
676 GCAGCTTCTGC
Bsu36I 109 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
Cfr10I 1502 ACCGGC [GA]^CCGG[CT]
1694 GCCGGC
DraIII 412 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
1861 CACAAGGTG
EarI 1212 CTCTTC CTCTTC(1/4)
964 GAAGAG
Eco81I 109 CCTGAGG CC^T[ACGT]AGG
1054 CCTGAGG
EcoT14I 554 CCATGG C^C[AT][AT]GG
1253 CCTTGG
Ksp632I 1212 CTCTTC CTCTTC(1/4)
964 GAAGAG
PflMI 1272 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1860 CCACAAGGTGG
StyI 554 CCATGG C^C[AT][AT]GG
1253 CCTTGG
TaqI 705 TCGA T^CGA
1239 TCGA
TthHB8I 705 TCGA T^CGA
1239 TCGA
Van91I 1272 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1860 CCACAAGGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI XcmI XhoII