Restriction map for
pF1KA1292
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI Aor51HI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BcgI BfaI BfrI BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FspI HpaI KpnI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 316 GTCTAC GT^[AC][GT]AC
AlwI 1140 GGATC GGATC(4/5)
AocI 618 CCTTAGG CC^T[ACGT]AGG
Asp700I 498 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1731 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1953 GAAGAC GAAGAC(2/6)
BclI 1755 TGATCA T^GATCA
BglII 2096 AGATCT A^GATCT
BlpI 1068 GCTCAGC GC^T[ACGT]AGC
BpmI 1626 CTCCAG CTGGAG(16/14)
Bpu1102I 1068 GCTCAGC GC^T[ACGT]AGC
BpuAI 1953 GAAGAC GAAGAC(2/6)
BsmAI 1631 GAGAC GTCTC(1/5)
BsmBI 1631 GAGACG CGTCTC(1/5)
BstAPI 1155 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1808 GGTCACC G^GT[ACGT]ACC
BstPI 1808 GGTCACC G^GT[ACGT]ACC
BstYI 2096 AGATCT [GA]^GATC[CT]
Bsu36I 618 CCTTAGG CC^T[ACGT]AGG
CelII 1068 GCTCAGC GC^T[ACGT]AGC
Eco81I 618 CCTTAGG CC^T[ACGT]AGG
EcoNI 1569 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1808 GGTCACC G^GT[ACGT]ACC
FbaI 1755 TGATCA T^GATCA
FseI 387 GGCCGGCC GGCCGG^CC
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
MflI 2096 AGATCT [GA]^GATC[CT]
NcoI 368 CCATGG C^CATGG
NspI 1041 GCATGC [GA]CATG^[CT]
PaeR7I 1122 CTCGAG C^TCGAG
PflFI 1731 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1300 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1948 CAGCTG CAG^CTG
SbfI 1746 CCTGCAGG CCTGCA^GG
SphI 1041 GCATGC GCATG^C
Sse8387I 1746 CCTGCAGG CCTGCA^GG
TfiI 949 GATTC G^A[AT]TC
Tth111I 1731 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1300 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1122 CTCGAG C^TCGAG
XhoII 2096 AGATCT [GA]^GATC[CT]
XmnI 498 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 1686 GACGTC GACGT^C
1731 GACGTC
AhdI 988 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACAACCTGTC
Alw44I 281 GTGCAC G^TGCAC
1721 GTGCAC
ApaLI 281 GTGCAC G^TGCAC
1721 GTGCAC
AspEI 988 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACAACCTGTC
BalI 2 TGGCCA TGG^CCA
1278 TGGCCA
BbeI 555 GGCGCC GGCGC^C
1663 GGCGCC
BbrPI 132 CACGTG CAC^GTG
535 CACGTG
BbvCI 1945 CCTCAGC CCTCAGC(-5/-2)
334 GCTGAGG
BciVI 1318 GGATAC GTATCC(6/5)
104 GTATCC
BseRI 1740 CTCCTC GAGGAG(10/8)
1486 GAGGAG
Bsp1407I 80 TGTACA T^GTACA
872 TGTACA
BsrGI 80 TGTACA T^GTACA
872 TGTACA
BstXI 5 CCACTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
362 CCATCACCATGG
DraIII 275 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
286 CACATGGTG
DrdI 1534 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1686 GACGTCACTGTC
Eam1105I 988 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACAACCTGTC
EarI 394 CTCTTC CTCTTC(1/4)
1525 CTCTTC
HindIII 661 AAGCTT A^AGCTT
1969 AAGCTT
KasI 555 GGCGCC G^GCGCC
1663 GGCGCC
Ksp632I 394 CTCTTC CTCTTC(1/4)
1525 CTCTTC
KspI 486 CCGCGG CCGC^GG
1726 CCGCGG
MluNI 2 TGGCCA TGG^CCA
1278 TGGCCA
MscI 2 TGGCCA TGG^CCA
1278 TGGCCA
MseI 1973 TTAA T^TAA
2060 TTAA
NarI 555 GGCGCC GG^CGCC
1663 GGCGCC
PmaCI 132 CACGTG CAC^GTG
535 CACGTG
PmlI 132 CACGTG CAC^GTG
535 CACGTG
SacII 486 CCGCGG CCGC^GG
1726 CCGCGG
SfoI 555 GGCGCC GGC^GCC
1663 GGCGCC
SspBI 80 TGTACA T^GTACA
872 TGTACA
StuI 622 AGGCCT AGG^CCT
1118 AGGCCT
TaqI 240 TCGA T^CGA
1123 TCGA
Tru9I 1973 TTAA T^TAA
2060 TTAA
TthHB8I 240 TCGA T^CGA
1123 TCGA
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BsaAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TseI
Tsp45I TspRI XcmI XmaCI XmaI