Restriction map for
pF1KB7757
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI HindIII KpnI MamI MfeI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfcI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 951 GAATTT [GA]^AATT[CT]
ApoI 951 GAATTT [GA]^AATT[CT]
Asp700I 987 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1146 GGTCC G^G[AT]CC
AviII 1130 TGCGCA TGC^GCA
BalI 704 TGGCCA TGG^CCA
BbrPI 1060 CACGTG CAC^GTG
BbsI 934 GTCTTC GAAGAC(2/6)
BfaI 754 CTAG C^TAG
BpuAI 934 GTCTTC GAAGAC(2/6)
BsaWI 522 ACCGGA [AT]^CCGG[AT]
BsgI 580 CTGCAC GTGCAG(16/14)
BsmBI 92 GAGACG CGTCTC(1/5)
BspLU11I 509 ACATGT A^CATGT
BssHII 940 GCGCGC G^CGCGC
BstAPI 1091 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 540 GGTGACC G^GT[ACGT]ACC
BstPI 540 GGTGACC G^GT[ACGT]ACC
BstYI 959 AGATCC [GA]^GATC[CT]
DraI 954 TTTAAA TTT^AAA
DraII 386 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 386 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 540 GGTGACC G^GT[ACGT]ACC
FseI 174 GGCCGGCC GGCCGG^CC
FspI 1130 TGCGCA TGC^GCA
HpaI 868 GTTAAC GTT^AAC
MaeI 754 CTAG C^TAG
MflI 959 AGATCC [GA]^GATC[CT]
MluI 458 ACGCGT A^CGCGT
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
PmaCI 1060 CACGTG CAC^GTG
PmlI 1060 CACGTG CAC^GTG
PvuI 32 CGATCG CGAT^CG
SacI 670 GAGCTC GAGCT^C
SfiI 388 GGCCCACACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 627 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 689 TCGA T^CGA
TspRI 1305 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 689 TCGA T^CGA
XcmI 854 CCAAATACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 959 AGATCC [GA]^GATC[CT]
XmnI 987 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 458 ACGCGT A^C[GA][CT]GT
509 ACATGT
Alw44I 883 GTGCAC G^TGCAC
1063 GTGCAC
AlwI 960 GATCC GGATC(4/5)
686 GGATC
ApaI 387 GGGCCC GGGCC^C
592 GGGCCC
ApaLI 883 GTGCAC G^TGCAC
1063 GTGCAC
BglI 251 GCCACGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
389 GCCCACACGGC
BsaAI 778 CACGTA [CT]AC^GT[GA]
1060 CACGTG
BsmAI 92 GAGAC GTCTC(1/5)
1230 GTCTC
BstF5I 876 CATCC GGATG(2/0)
1117 CATCC
EarI 424 CTCTTC CTCTTC(1/4)
1314 CTCTTC
FokI 876 CATCC GGATG(9/13)
1117 CATCC
HincII 475 GTCAAC GT[CT]^[GA]AC
868 GTTAAC
HindII 475 GTCAAC GT[CT]^[GA]AC
868 GTTAAC
HinfI 1153 GAATC G^A[ACGT]TC
1204 GAATC
Ksp632I 424 CTCTTC CTCTTC(1/4)
1314 CTCTTC
KspI 71 CCGCGG CCGC^GG
410 CCGCGG
PvuII 577 CAGCTG CAG^CTG
700 CAGCTG
SacII 71 CCGCGG CCGC^GG
410 CCGCGG
SmaI 246 CCCGGG CCC^GGG
430 CCCGGG
TfiI 1153 GAATC G^A[AT]TC
1204 GAATC
Tsp509I 952 AATT ^AATT
1321 AATT
XmaCI 246 CCCGGG C^CCGGG
430 CCCGGG
XmaI 246 CCCGGG C^CCGGG
430 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanI BanII BbeI
BbvI BcgI BcnI BmyI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BssSI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
Tru9I TseI Tsp45I