################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MspA1I MunI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 317 TCCGGA T^CCGGA AcsI 120 GAATTC [GA]^AATT[CT] AflII 1264 CTTAAG C^TTAAG AlwI 448 GATCC GGATC(4/5) AlwNI 951 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 120 GAATTC [GA]^AATT[CT] BbvI 328 GCAGC GCAGC(8/12) BciVI 489 GGATAC GTATCC(6/5) BfrI 1264 CTTAAG C^TTAAG BseAI 317 TCCGGA T^CCGGA BseRI 1231 GAGGAG GAGGAG(10/8) BsmFI 60 GTCCC GGGAC(10/14) BspEI 317 TCCGGA T^CCGGA BspHI 903 TCATGA T^CATGA BspMI 1081 GCAGGT ACCTGC(4/8) BstF5I 1380 GGATG GGATG(2/0) BstYI 447 AGATCC [GA]^GATC[CT] DdeI 620 CTCAG C^T[ACGT]AG EcoRI 120 GAATTC G^AATTC Fnu4HI 328 GCAGC GC^[ACGT]GC FokI 1380 GGATG GGATG(9/13) HincII 941 GTCAAC GT[CT]^[GA]AC HindII 941 GTCAAC GT[CT]^[GA]AC HindIII 1261 AAGCTT A^AGCTT ItaI 328 GCAGC GC^[ACGT]GC MaeII 1317 ACGT A^CGT MflI 447 AGATCC [GA]^GATC[CT] MroI 317 TCCGGA T^CCGGA MwoI 1152 GCAGTCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 65 ACATGC [GA]CATG^[CT] RcaI 903 TCATGA T^CATGA SfcI 749 CTGTAG C^T[GA][CT]AG SspI 340 AATATT AAT^ATT TseI 328 GCAGC G^C[AT]GC XhoII 447 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbsI 129 GAAGAC GAAGAC(2/6) 381 GAAGAC BpuAI 129 GAAGAC GAAGAC(2/6) 381 GAAGAC BsaWI 56 TCCGGT [AT]^CCGG[AT] 317 TCCGGA BsrDI 889 CATTGC GCAATG(2/0) 352 GCAATG DpnII 448 GATC ^GATC 530 GATC HaeIII 198 GGCC GG^CC 1139 GGCC HapII 57 CCGG C^CGG 318 CCGG HpaII 57 CCGG C^CGG 318 CCGG HphI 598 TCACC GGTGA(8/7) 947 TCACC MboI 448 GATC ^GATC 530 GATC MslI 168 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 684 CATAAAAGTG MspI 57 CCGG C^CGG 318 CCGG NdeI 386 CATATG CA^TATG 650 CATATG NdeII 448 GATC ^GATC 530 GATC NlaIV 59 GGTCCC GG[ACGT]^[ACGT]CC 523 GGACCC Sau3AI 448 GATC ^GATC 530 GATC SfaNI 1211 GCATC GCATC(5/9) 1269 GCATC SmlI 981 CTTGAG C^T[CT][GA]AG 1264 CTTAAG TaqI 263 TCGA T^CGA 1272 TCGA Tsp509I 87 AATT ^AATT 121 AATT TthHB8I 263 TCGA T^CGA 1272 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AfaI AluI AvaII BfaI BsgI BsmAI BsrI Cfr13I EarI HinfI Ksp632I MaeI MaeIII MboII MnlI MseI NlaIII PleI RsaI Sau96I TfiI Tru9I TspRI