Restriction map for pF1KA1789
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MscI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII        317  TCCGGA               T^CCGGA
AcsI          120  GAATTC               [GA]^AATT[CT]
AflII        1264  CTTAAG               C^TTAAG
AlwI          448  GATCC                GGATC(4/5)
AlwNI         951  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          120  GAATTC               [GA]^AATT[CT]
BbvI          328  GCAGC                GCAGC(8/12)
BciVI         489  GGATAC               GTATCC(6/5)
BfrI         1264  CTTAAG               C^TTAAG
BseAI         317  TCCGGA               T^CCGGA
BseRI        1231  GAGGAG               GAGGAG(10/8)
BsmFI          60  GTCCC                GGGAC(10/14)
BspEI         317  TCCGGA               T^CCGGA
BspHI         903  TCATGA               T^CATGA
BspMI        1081  GCAGGT               ACCTGC(4/8)
BstF5I       1380  GGATG                GGATG(2/0)
BstYI         447  AGATCC               [GA]^GATC[CT]
DdeI          620  CTCAG                C^T[ACGT]AG
EcoRI         120  GAATTC               G^AATTC
Fnu4HI        328  GCAGC                GC^[ACGT]GC
FokI         1380  GGATG                GGATG(9/13)
HincII        941  GTCAAC               GT[CT]^[GA]AC
HindII        941  GTCAAC               GT[CT]^[GA]AC
HindIII      1261  AAGCTT               A^AGCTT
ItaI          328  GCAGC                GC^[ACGT]GC
MaeII        1317  ACGT                 A^CGT
MflI          447  AGATCC               [GA]^GATC[CT]
MroI          317  TCCGGA               T^CCGGA
MwoI         1152  GCAGTCCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI           65  ACATGC               [GA]CATG^[CT]
RcaI          903  TCATGA               T^CATGA
SfcI          749  CTGTAG               C^T[GA][CT]AG
SspI          340  AATATT               AAT^ATT
TseI          328  GCAGC                G^C[AT]GC
XhoII         447  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbsI          129  GAAGAC               GAAGAC(2/6)
              381  GAAGAC              
BpuAI         129  GAAGAC               GAAGAC(2/6)
              381  GAAGAC              
BsaWI          56  TCCGGT               [AT]^CCGG[AT]
              317  TCCGGA              
BsrDI         889  CATTGC               GCAATG(2/0)
              352  GCAATG              
DpnII         448  GATC                 ^GATC
              530  GATC                
HaeIII        198  GGCC                 GG^CC
             1139  GGCC                
HapII          57  CCGG                 C^CGG
              318  CCGG                
HpaII          57  CCGG                 C^CGG
              318  CCGG                
HphI          598  TCACC                GGTGA(8/7)
              947  TCACC               
MboI          448  GATC                 ^GATC
              530  GATC                
MslI          168  CATCAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              684  CATAAAAGTG          
MspI           57  CCGG                 C^CGG
              318  CCGG                
NdeI          386  CATATG               CA^TATG
              650  CATATG              
NdeII         448  GATC                 ^GATC
              530  GATC                
NlaIV          59  GGTCCC               GG[ACGT]^[ACGT]CC
              523  GGACCC              
Sau3AI        448  GATC                 ^GATC
              530  GATC                
SfaNI        1211  GCATC                GCATC(5/9)
             1269  GCATC               
SmlI          981  CTTGAG               C^T[CT][GA]AG
             1264  CTTAAG              
TaqI          263  TCGA                 T^CGA
             1272  TCGA                
Tsp509I        87  AATT                 ^AATT
              121  AATT                
TthHB8I       263  TCGA                 T^CGA
             1272  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AfaI        AluI        AvaII       BfaI        BsgI        
BsmAI       BsrI        Cfr13I      EarI        HinfI       
Ksp632I     MaeI        MaeIII      MboII       MnlI        
MseI        NlaIII      PleI        RsaI        Sau96I      
TfiI        Tru9I       TspRI       
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