Restriction map for
pF1KB6810
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HaeIII HgaI Hin1I
HincII HindII HindIII HpaI HphI
ItaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
TseI Tsp509I Van91I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 360 GCGG CCGC(-3/-1)
AfaI 111 GTAC GT^AC
AspHI 391 GAGCTC G[AT]GC[AT]^C
AspI 433 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 341 CTCGAG C^[CT]CG[GA]G
AvaII 438 GGTCC G^G[AT]CC
BanII 391 GAGCTC G[GA]GC[CT]^C
BcgI 183 CGACCTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 40 CTAG C^TAG
BpmI 381 CTCCAG CTGGAG(16/14)
BsiEI 81 CGACCG CG[GA][CT]^CG
BsiHKAI 391 GAGCTC G[AT]GC[AT]^C
BsmAI 226 GAGAC GTCTC(1/5)
BsmFI 439 GTCCC GGGAC(10/14)
BsmI 510 GCATTC GAATGC(1/-1)
BsoBI 341 CTCGAG C^[CT]CG[GA]G
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BsrDI 189 CATTGC GCAATG(2/0)
BsrI 383 CCAGT ACTGG(1/-1)
BssSI 329 CACGAG CACGAG(-5/-1)
BstF5I 528 GGATG GGATG(2/0)
Cac8I 248 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 252 GCGC GCG^C
Cfr13I 438 GGTCC G^G[ACGT]CC
EcoT14I 446 CCAAGG C^C[AT][AT]GG
FokI 528 GGATG GGATG(9/13)
HapII 355 CCGG C^CGG
HhaI 252 GCGC GCG^C
HinP1I 252 GCGC G^CGC
HpaII 355 CCGG C^CGG
MaeI 40 CTAG C^TAG
MaeII 505 ACGT A^CGT
MaeIII 207 GTCAC ^GT[ACGT]AC
MspI 355 CCGG C^CGG
PaeR7I 341 CTCGAG C^TCGAG
PflFI 433 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 202 CTGCAG CTGCA^G
RsaI 111 GTAC GT^AC
SacI 391 GAGCTC GAGCT^C
Sau96I 438 GGTCC G^G[ACGT]CC
SexAI 434 ACCTGGT A^CC[AT]GGT
SmlI 341 CTCGAG C^T[CT][GA]AG
StyI 446 CCAAGG C^C[AT][AT]GG
Tsp45I 207 GTCAC ^GT[GC]AC
TspRI 384 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 433 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 341 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
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Asp700I 70 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
126 GAAGGGATTC
BmyI 391 GAGCTC G[AGT]GC[ACT]^C
465 GTGCCC
BsaJI 446 CCAAGG C^C[ACGT][ACGT]GG
521 CCTCGG
Bsp1286I 391 GAGCTC G[AGT]GC[ACT]^C
465 GTGCCC
BssKI 171 CCTGG ^CC[ACGT]GG
435 CCTGG
BstNI 171 CCTGG CC^[AT]GG
435 CCTGG
DdeI 135 CTCAG C^T[ACGT]AG
194 CTGAG
EcoRII 171 CCTGG ^CC[AT]GG
435 CCTGG
MvaI 171 CCTGG CC^[AT]GG
435 CCTGG
MwoI 445 GCCAAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
501 GCTGACGTGGC
PleI 379 GACTC GAGTC(4/5)
316 GAGTC
ScrFI 171 CCTGG CC^[ACGT]GG
435 CCTGG
SfcI 202 CTGCAG C^T[GA][CT]AG
374 CTACAG
XmnI 70 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
126 GAAGGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BseRI BsiYI BslI EarI
HinfI Ksp632I MboII MnlI NlaIII
NlaIV TaqI TfiI TthHB8I