##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NlaIV       NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AfaI         1046  GTAC                 GT^AC
AlwI           64  GATCC                GGATC(4/5)
AlwNI         268  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
BciVI        1124  GTATCC               GTATCC(6/5)
BsaAI         422  CACGTA               [CT]AC^GT[GA]
BseRI          46  GAGGAG               GAGGAG(10/8)
BsiYI         122  CCTCTATAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          122  CCTCTATAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI        1560  GAGAC                GTCTC(1/5)
BspLU11I     1565  ACATGT               A^CATGT
DraI          838  TTTAAA               TTT^AAA
DraII        1167  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        566  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I     1167  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      1602  ATGCAT               ATGCA^T
HincII       1569  GTTAAC               GT[CT]^[GA]AC
HindII       1569  GTTAAC               GT[CT]^[GA]AC
HpaI         1569  GTTAAC               GTT^AAC
MaeII         423  ACGT                 A^CGT
MwoI         1544  GCCGTGCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          196  CATATG               CA^TATG
NsiI         1602  ATGCAT               ATGCA^T
PpuMI        1167  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI         1046  GTAC                 GT^AC
StuI          944  AGGCCT               AGG^CCT
Tsp45I        650  GTCAC                ^GT[GC]AC
XcmI          107  CCATGAGGAATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AflIII        421  ACACGT               A^C[GA][CT]GT
             1565  ACATGT              
BfaI           61  CTAG                 C^TAG
             1463  CTAG                
BglII        1036  AGATCT               A^GATCT
             1456  AGATCT              
BsmI          925  GAATGC               GAATGC(1/-1)
             1114  GCATTC              
BssKI          42  CCAGG                ^CC[ACGT]GG
              289  CCTGG               
BstNI          42  CCAGG                CC^[AT]GG
              289  CCTGG               
Cac8I         929  GCAAGC               GC[ACGT]^[ACGT]GC
             1549  GCCAGC              
EcoRI         815  GAATTC               G^AATTC
              983  GAATTC              
EcoRII         42  CCAGG                ^CC[AT]GG
              289  CCTGG               
HaeIII        120  GGCC                 GG^CC
              945  GGCC                
HindIII       608  AAGCTT               A^AGCTT
             1280  AAGCTT              
MaeI           61  CTAG                 C^TAG
             1463  CTAG                
MaeIII        425  GTAAC                ^GT[ACGT]AC
              650  GTCAC               
MvaI           42  CCAGG                CC^[AT]GG
              289  CCTGG               
ScrFI          42  CCAGG                CC^[ACGT]GG
              289  CCTGG               
SfcI           84  CTACAG               C^T[GA][CT]AG
              125  CTATAG              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     AvaII       
BbsI        BpmI        BpuAI       BsaJI       BsrDI       
BsrI        BstBI       BstF5I      BstYI       Cfr13I      
DdeI        DpnII       FokI        HinfI       HphI        
MboI        MboII       MflI        MnlI        MseI        
MslI        NdeII       NlaIII      NspI        NspV        
PleI        Sau3AI      Sau96I      SfuI        TaqI        
TfiI        Tru9I       Tsp509I     TspRI       TthHB8I     
XhoII       XmnI