Restriction map for pF1KB7633
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbvCI       BcgI        
BciVI       BcnI        BfaI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1407I    BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      CpoI        
DraI        DraII       DraIII      DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MluI        MroI        MspI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AfaI          841  GTAC                 GT^AC
AlwI          198  GGATC                GGATC(4/5)
AocI          834  CCTGAGG              CC^T[ACGT]AGG
AspI          176  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          93  GGACC                G^G[AT]CC
BalI          945  TGGCCA               TGG^CCA
BanII         489  GAGCTC               G[GA]GC[CT]^C
BbrPI         844  CACGTG               CAC^GTG
BbvI            4  GCTGC                GCAGC(8/12)
BclI          462  TGATCA               T^GATCA
BglII         430  AGATCT               A^GATCT
BsaAI         844  CACGTG               [CT]AC^GT[GA]
BseRI          76  GAGGAG               GAGGAG(10/8)
BsgI          248  GTGCAG               GTGCAG(16/14)
BspDI         652  ATCGAT               AT^CGAT
BspLU11I      591  ACATGT               A^CATGT
BsrDI          86  CATTGC               GCAATG(2/0)
BstYI         430  AGATCT               [GA]^GATC[CT]
Bsu36I        834  CCTGAGG              CC^T[ACGT]AGG
Cfr13I         93  GGACC                G^G[ACGT]CC
ClaI          652  ATCGAT               AT^CGAT
DrdI           18  GACAACCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          945  TGGCCA               [CT]^GGCC[GA]
Eco81I        834  CCTGAGG              CC^T[ACGT]AGG
EcoRI         822  GAATTC               G^AATTC
EcoT14I       191  CCAAGG               C^C[AT][AT]GG
FbaI          462  TGATCA               T^GATCA
Fnu4HI          4  GCTGC                GC^[ACGT]GC
HinfI         935  GAATC                G^A[ACGT]TC
ItaI            4  GCTGC                GC^[ACGT]GC
MaeII         845  ACGT                 A^CGT
MfeI          284  CAATTG               C^AATTG
MflI          430  AGATCT               [GA]^GATC[CT]
MluNI         945  TGGCCA               TGG^CCA
MscI          945  TGGCCA               TGG^CCA
MspA1I        208  CAGCTG               C[AC]G^C[GT]G
MunI          284  CAATTG               C^AATTG
NspI          591  ACATGT               [GA]CATG^[CT]
PflFI         176  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          48  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         844  CACGTG               CAC^GTG
PmlI          844  CACGTG               CAC^GTG
PvuII         208  CAGCTG               CAG^CTG
RsaI          841  GTAC                 GT^AC
SacI          489  GAGCTC               GAGCT^C
Sau96I         93  GGACC                G^G[ACGT]CC
SexAI          22  ACCTGGT              A^CC[AT]GGT
StuI          812  AGGCCT               AGG^CCT
StyI          191  CCAAGG               C^C[AT][AT]GG
TaqI          653  TCGA                 T^CGA
TfiI          935  GAATC                G^A[AT]TC
TseI            4  GCTGC                G^C[AT]GC
Tth111I       176  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       653  TCGA                 T^CGA
Van91I         48  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         430  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          230  GAATTT               [GA]^AATT[CT]
              822  GAATTC              
AflII         415  CTTAAG               C^TTAAG
              667  CTTAAG              
AflIII        591  ACATGT               A^C[GA][CT]GT
              843  ACACGT              
Alw44I        505  GTGCAC               G^TGCAC
              757  GTGCAC              
AlwNI         378  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              630  CAGTGACTG           
ApaLI         505  GTGCAC               G^TGCAC
              757  GTGCAC              
ApoI          230  GAATTT               [GA]^AATT[CT]
              822  GAATTC              
Asp700I       390  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              558  GAAGGCTTTC          
BbsI          213  GAAGAC               GAAGAC(2/6)
              408  GTCTTC              
BfrI          415  CTTAAG               C^TTAAG
              667  CTTAAG              
BpuAI         213  GAAGAC               GAAGAC(2/6)
              408  GTCTTC              
BsiYI          48  CCAGGAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              152  CCTCAGTAGGG         
BslI           48  CCAGGAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              152  CCTCAGTAGGG         
BsmI          373  GAATGC               GAATGC(1/-1)
              625  GAATGC              
HphI          241  GGTGA                GGTGA(8/7)
              778  GGTGA               
MboII         213  GAAGA                GAAGA(8/7)
              409  TCTTC               
MslI          496  CATCTGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              772  CACACTGGTG          
NlaIV          93  GGACCC               GG[ACGT]^[ACGT]CC
              143  GGAACC              
PstI            5  CTGCAG               CTGCA^G
              171  CTGCAG              
SfaNI         126  GATGC                GCATC(5/9)
              363  GATGC               
SfcI            5  CTGCAG               C^T[GA][CT]AG
              171  CTGCAG              
SmlI          415  CTTAAG               C^T[CT][GA]AG
              667  CTTAAG              
XmnI          390  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              558  GAAGGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        AspHI       BmyI        BpmI        BsaJI       
BsiHKAI     Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       DdeI        DpnII       EcoRII      FokI        
HaeIII      MaeIII      MboI        MnlI        MseI        
MvaI        NdeII       NlaIII      Sau3AI      ScrFI       
Tru9I       Tsp45I      Tsp509I     TspRI       
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