################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BcnI BfaI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MluI MroI MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AfaI 841 GTAC GT^AC AlwI 198 GGATC GGATC(4/5) AocI 834 CCTGAGG CC^T[ACGT]AGG AspI 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 93 GGACC G^G[AT]CC BalI 945 TGGCCA TGG^CCA BanII 489 GAGCTC G[GA]GC[CT]^C BbrPI 844 CACGTG CAC^GTG BbvI 4 GCTGC GCAGC(8/12) BclI 462 TGATCA T^GATCA BglII 430 AGATCT A^GATCT BsaAI 844 CACGTG [CT]AC^GT[GA] BseRI 76 GAGGAG GAGGAG(10/8) BsgI 248 GTGCAG GTGCAG(16/14) BspDI 652 ATCGAT AT^CGAT BspLU11I 591 ACATGT A^CATGT BsrDI 86 CATTGC GCAATG(2/0) BstYI 430 AGATCT [GA]^GATC[CT] Bsu36I 834 CCTGAGG CC^T[ACGT]AGG Cfr13I 93 GGACC G^G[ACGT]CC ClaI 652 ATCGAT AT^CGAT DrdI 18 GACAACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 945 TGGCCA [CT]^GGCC[GA] Eco81I 834 CCTGAGG CC^T[ACGT]AGG EcoRI 822 GAATTC G^AATTC EcoT14I 191 CCAAGG C^C[AT][AT]GG FbaI 462 TGATCA T^GATCA Fnu4HI 4 GCTGC GC^[ACGT]GC HinfI 935 GAATC G^A[ACGT]TC ItaI 4 GCTGC GC^[ACGT]GC MaeII 845 ACGT A^CGT MfeI 284 CAATTG C^AATTG MflI 430 AGATCT [GA]^GATC[CT] MluNI 945 TGGCCA TGG^CCA MscI 945 TGGCCA TGG^CCA MspA1I 208 CAGCTG C[AC]G^C[GT]G MunI 284 CAATTG C^AATTG NspI 591 ACATGT [GA]CATG^[CT] PflFI 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 48 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 844 CACGTG CAC^GTG PmlI 844 CACGTG CAC^GTG PvuII 208 CAGCTG CAG^CTG RsaI 841 GTAC GT^AC SacI 489 GAGCTC GAGCT^C Sau96I 93 GGACC G^G[ACGT]CC SexAI 22 ACCTGGT A^CC[AT]GGT StuI 812 AGGCCT AGG^CCT StyI 191 CCAAGG C^C[AT][AT]GG TaqI 653 TCGA T^CGA TfiI 935 GAATC G^A[AT]TC TseI 4 GCTGC G^C[AT]GC Tth111I 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 653 TCGA T^CGA Van91I 48 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 430 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 230 GAATTT [GA]^AATT[CT] 822 GAATTC AflII 415 CTTAAG C^TTAAG 667 CTTAAG AflIII 591 ACATGT A^C[GA][CT]GT 843 ACACGT Alw44I 505 GTGCAC G^TGCAC 757 GTGCAC AlwNI 378 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 630 CAGTGACTG ApaLI 505 GTGCAC G^TGCAC 757 GTGCAC ApoI 230 GAATTT [GA]^AATT[CT] 822 GAATTC Asp700I 390 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 558 GAAGGCTTTC BbsI 213 GAAGAC GAAGAC(2/6) 408 GTCTTC BfrI 415 CTTAAG C^TTAAG 667 CTTAAG BpuAI 213 GAAGAC GAAGAC(2/6) 408 GTCTTC BsiYI 48 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 152 CCTCAGTAGGG BslI 48 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 152 CCTCAGTAGGG BsmI 373 GAATGC GAATGC(1/-1) 625 GAATGC HphI 241 GGTGA GGTGA(8/7) 778 GGTGA MboII 213 GAAGA GAAGA(8/7) 409 TCTTC MslI 496 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 772 CACACTGGTG NlaIV 93 GGACCC GG[ACGT]^[ACGT]CC 143 GGAACC PstI 5 CTGCAG CTGCA^G 171 CTGCAG SfaNI 126 GATGC GCATC(5/9) 363 GATGC SfcI 5 CTGCAG C^T[GA][CT]AG 171 CTGCAG SmlI 415 CTTAAG C^T[CT][GA]AG 667 CTTAAG XmnI 390 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 558 GAAGGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AspHI BmyI BpmI BsaJI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HaeIII MaeIII MboI MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI Tru9I Tsp45I Tsp509I TspRI