Restriction map for
pF1KB7826
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I HindIII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI MwoI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 742 AACGTT AA^CGTT
AcyI 340 GGCGTC G[GA]^CG[CT]C
AflIII 817 ACATGT A^C[GA][CT]GT
AlwNI 464 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 89 GGACC G^G[AT]CC
BbsI 1344 GAAGAC GAAGAC(2/6)
BciVI 144 GTATCC GTATCC(6/5)
BclI 636 TGATCA T^GATCA
BcnI 1609 CCGGG CC^[GC]GG
BpuAI 1344 GAAGAC GAAGAC(2/6)
BsaHI 340 GGCGTC G[GA]^CG[CT]C
BsaI 132 GGTCTC GGTCTC(1/5)
BseRI 58 GAGGAG GAGGAG(10/8)
BsmI 1069 GAATGC GAATGC(1/-1)
BspLU11I 817 ACATGT A^CATGT
BspMI 438 GCAGGT ACCTGC(4/8)
BstYI 227 GGATCT [GA]^GATC[CT]
Cac8I 901 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 89 GGACC G^G[ACGT]CC
DraII 88 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1795 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 196 CCATGG C^C[GA][CT]GG
EcoNI 1334 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 88 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 196 CCATGG C^C[AT][AT]GG
FbaI 636 TGATCA T^GATCA
HapII 1609 CCGG C^CGG
HgaI 341 GCGTC GACGC(5/10)
Hin1I 340 GGCGTC G[GA]^CG[CT]C
HincII 451 GTTAAC GT[CT]^[GA]AC
HindII 451 GTTAAC GT[CT]^[GA]AC
HpaI 451 GTTAAC GTT^AAC
HpaII 1609 CCGG C^CGG
MaeII 743 ACGT A^CGT
MflI 227 GGATCT [GA]^GATC[CT]
MspI 1609 CCGG C^CGG
NciI 1609 CCGGG CC^[GC]GG
NcoI 196 CCATGG C^CATGG
NlaIV 89 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 88 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 742 AACGTT AA^CGTT
PstI 400 CTGCAG CTGCA^G
SacI 1202 GAGCTC GAGCT^C
SapI 190 GAAGAGC GCTCTTC(1/4)
Sau96I 89 GGACC G^G[ACGT]CC
SexAI 128 ACCTGGT A^CC[AT]GGT
SmlI 691 CTTGAG C^T[CT][GA]AG
SphI 901 GCATGC GCATG^C
StyI 196 CCATGG C^C[AT][AT]GG
XbaI 411 TCTAGA T^CTAGA
XhoII 227 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 200 GGATC GGATC(4/5)
227 GGATC
BanII 676 GAGCCC G[GA]GC[CT]^C
1202 GAGCTC
BbvI 66 GCAGC GCAGC(8/12)
399 GCTGC
BfaI 412 CTAG C^TAG
665 CTAG
BsaBI 238 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
724 GATGATAATC
DraI 372 TTTAAA TTT^AAA
568 TTTAAA
FauI 1424 CCCGC CCCGC(4/6)
1073 GCGGG
Fnu4HI 66 GCAGC GC^[ACGT]GC
399 GCTGC
ItaI 66 GCAGC GC^[ACGT]GC
399 GCTGC
MaeI 412 CTAG C^TAG
665 CTAG
MamI 238 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
724 GATGATAATC
MslI 553 CATAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1291 CACACTGGTG
MspA1I 397 CAGCTG C[AC]G^C[GT]G
1183 CAGCTG
NdeI 887 CATATG CA^TATG
1647 CATATG
NspI 817 ACATGT [GA]CATG^[CT]
901 GCATGC
PvuII 397 CAGCTG CAG^CTG
1183 CAGCTG
SfcI 120 CTACAG C^T[GA][CT]AG
400 CTGCAG
SspI 507 AATATT AAT^ATT
589 AATATT
TseI 66 GCAGC G^C[AT]GC
399 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI DdeI DpnII EarI
EcoRI EcoRII FokI HaeIII HinfI
HphI Ksp632I MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI SspBI
StuI TfiI Tru9I Tsp45I Tsp509I
TspRI XmnI