Restriction map for
pF1KB7857
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeIII MamI MfeI MroI
MunI NcoI NdeI NheI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 888 GACGTC GACGT^C
AflIII 938 ACGCGT A^C[GA][CT]GT
AviII 1111 TGCGCA TGC^GCA
BamHI 1502 GGATCC G^GATCC
BbeI 1393 GGCGCC GGCGC^C
BbvCI 1065 CCTCAGC CCTCAGC(-5/-2)
BlpI 1404 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1404 GCTCAGC GC^T[ACGT]AGC
BsiWI 1165 CGTACG C^GTACG
BssHII 1196 GCGCGC G^CGCGC
BstBI 769 TTCGAA TT^CGAA
BstXI 1086 CCACGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1404 GCTCAGC GC^T[ACGT]AGC
DraIII 1115 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 218 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1388 CCAAGG C^C[AT][AT]GG
FspI 1111 TGCGCA TGC^GCA
KasI 1393 GGCGCC G^GCGCC
MaeII 889 ACGT A^CGT
MluI 938 ACGCGT A^CGCGT
MseI 1238 TTAA T^TAA
NaeI 646 GCCGGC GCC^GGC
NarI 1393 GGCGCC GG^CGCC
NgoMIV 646 GCCGGC G^CCGGC
NotI 1289 GCGGCCGC GC^GGCCGC
NspI 543 GCATGC [GA]CATG^[CT]
NspV 769 TTCGAA TT^CGAA
PflMI 630 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 364 CTGCAG CTGCA^G
SacI 804 GAGCTC GAGCT^C
SfoI 1393 GGCGCC GGC^GCC
SfuI 769 TTCGAA TT^CGAA
SgrAI 1411 CACCGGCG C[GA]^CCGG[CT]G
SmlI 817 CTCAAG C^T[CT][GA]AG
SphI 543 GCATGC GCATG^C
StyI 1388 CCAAGG C^C[AT][AT]GG
Tru9I 1238 TTAA T^TAA
Van91I 630 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 153 CCAACTGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 593 GTGCAC G^TGCAC
1252 GTGCAC
AlwNI 631 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1330 CAGCTCCTG
ApaI 68 GGGCCC GGGCC^C
1626 GGGCCC
ApaLI 593 GTGCAC G^TGCAC
1252 GTGCAC
BalI 280 TGGCCA TGG^CCA
708 TGGCCA
BbsI 1518 GAAGAC GAAGAC(2/6)
325 GTCTTC
BcgI 788 GCAAAGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1376 GCAAGGCCTTCG
BglI 61 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
573 GCCTGAGCGGC
BpuAI 1518 GAAGAC GAAGAC(2/6)
325 GTCTTC
BsmBI 1638 CGTCTC CGTCTC(1/5)
928 GAGACG
BsmI 772 GAATGC GAATGC(1/-1)
1418 GCATTC
BstAPI 543 GCATGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
776 GCAGGGCGTGC
BstYI 1082 AGATCC [GA]^GATC[CT]
1502 GGATCC
DsaI 407 CCACGG C^C[GA][CT]GG
1053 CCACGG
EagI 1007 CGGCCG C^GGCCG
1290 CGGCCG
EclXI 1007 CGGCCG C^GGCCG
1290 CGGCCG
Eco52I 1007 CGGCCG C^GGCCG
1290 CGGCCG
HinfI 122 GACTC G^A[ACGT]TC
698 GACTC
MflI 1082 AGATCC [GA]^GATC[CT]
1502 GGATCC
MluNI 280 TGGCCA TGG^CCA
708 TGGCCA
MscI 280 TGGCCA TGG^CCA
708 TGGCCA
MslI 408 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1087 CACGCGGGTG
NlaIII 544 CATG CATG^
590 CATG
PleI 122 GACTC GAGTC(4/5)
698 GACTC
PpuMI 602 AGGTCCC [GA]G^G[AT]CC[CT]
632 AGGACCT
SapI 801 GAAGAGC GCTCTTC(1/4)
745 GCTCTTC
SfcI 219 CTACAG C^T[GA][CT]AG
364 CTGCAG
XhoII 1082 AGATCC [GA]^GATC[CT]
1502 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
TaqI TseI TspRI TthHB8I XmnI