Restriction map for
pF1KB7815
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV FauI
FseI FspI HindIII KasI KpnI
KspI MaeI MamI MflI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 1427 GCGG CCGC(-3/-1)
AcyI 1029 GACGCC G[GA]^CG[CT]C
AflIII 991 ACGTGT A^C[GA][CT]GT
AlwI 3 GATCC GGATC(4/5)
AlwNI 355 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1487 CCTCAGG CC^T[ACGT]AGG
Aor51HI 266 AGCGCT AGC^GCT
AvaII 90 GGACC G^G[AT]CC
BbsI 987 GAAGAC GAAGAC(2/6)
BcnI 99 CCGGG CC^[GC]GG
BpuAI 987 GAAGAC GAAGAC(2/6)
BsaAI 392 TACGTG [CT]AC^GT[GA]
BsaHI 1029 GACGCC G[GA]^CG[CT]C
Bsp1407I 918 TGTACA T^GTACA
BsrGI 918 TGTACA T^GTACA
BstXI 20 CCATAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1487 CCTCAGG CC^T[ACGT]AGG
DraII 89 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 169 CTCTTC CTCTTC(1/4)
Eco47III 266 AGCGCT AGC^GCT
Eco81I 1487 CCTCAGG CC^T[ACGT]AGG
EcoO109I 89 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1295 CCAAGG C^C[AT][AT]GG
HgaI 1029 GACGC GACGC(5/10)
Hin1I 1029 GACGCC G[GA]^CG[CT]C
HincII 427 GTTAAC GT[CT]^[GA]AC
HindII 427 GTTAAC GT[CT]^[GA]AC
HpaI 427 GTTAAC GTT^AAC
Ksp632I 169 CTCTTC CTCTTC(1/4)
MfeI 203 CAATTG C^AATTG
MspA1I 1425 CAGCGG C[AC]G^C[GT]G
MunI 203 CAATTG C^AATTG
MwoI 1369 GCTAACGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 99 CCGGG CC^[GC]GG
NdeI 240 CATATG CA^TATG
PpuMI 89 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1313 CTGCAG CTGCA^G
SmlI 814 CTCAAG C^T[CT][GA]AG
SspBI 918 TGTACA T^GTACA
SspI 331 AATATT AAT^ATT
StyI 1295 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 96 GTAC GT^AC
919 GTAC
AluI 1163 AGCT AG^CT
1327 AGCT
BsaI 1197 GAGACC GGTCTC(1/5)
1078 GGTCTC
BsaWI 159 ACCGGA [AT]^CCGG[AT]
853 ACCGGA
BseRI 1432 CTCCTC GAGGAG(10/8)
58 GAGGAG
BsmI 787 GAATGC GAATGC(1/-1)
1530 GAATGC
BspHI 309 TCATGA T^CATGA
375 TCATGA
BsrDI 340 CATTGC GCAATG(2/0)
959 GCAATG
Cac8I 151 GCCAGC GC[ACGT]^[ACGT]GC
269 GCTTGC
CfoI 77 GCGC GCG^C
267 GCGC
EcoRI 929 GAATTC G^AATTC
1097 GAATTC
EcoT22I 320 ATGCAT ATGCA^T
679 ATGCAT
HaeII 76 GGCGCT [GA]GCGC^[CT]
266 AGCGCT
HhaI 77 GCGC GCG^C
267 GCGC
HinP1I 77 GCGC G^CGC
267 GCGC
MaeIII 1211 GTAAC ^GT[ACGT]AC
1582 GTTAC
NlaIV 226 GGAGCC GG[ACGT]^[ACGT]CC
1401 GGCCCC
NsiI 320 ATGCAT ATGCA^T
679 ATGCAT
PleI 484 GACTC GAGTC(4/5)
1182 GACTC
RcaI 309 TCATGA T^CATGA
375 TCATGA
RsaI 96 GTAC GT^AC
919 GTAC
SexAI 128 ACCTGGT A^CC[AT]GGT
671 ACCAGGT
SfcI 120 CTACAG C^T[GA][CT]AG
1313 CTGCAG
TfiI 16 GAATC G^A[AT]TC
215 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI ApoI Asp700I BbvI BclI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MseI MslI
MspI MvaI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp509I TspRI XmnI