Restriction map for
pF1KB7836
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspEI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 241 GGCGTC G[GA]^CG[CT]C
AflII 1338 CTTAAG C^TTAAG
AspI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 869 GGACC G^G[AT]CC
BbsI 514 GTCTTC GAAGAC(2/6)
BcnI 117 CCGGG CC^[GC]GG
BfrI 1338 CTTAAG C^TTAAG
BpuAI 514 GTCTTC GAAGAC(2/6)
BsaHI 241 GGCGTC G[GA]^CG[CT]C
BsaI 150 GGTCTC GGTCTC(1/5)
BsaWI 1213 ACCGGA [AT]^CCGG[AT]
BseRI 246 CTCCTC GAGGAG(10/8)
BsgI 1601 GTGCAG GTGCAG(16/14)
BsiYI 873 CCATACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 873 CCATACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 243 CGTCTC CGTCTC(1/5)
BsmFI 159 GGGAC GGGAC(10/14)
BsmI 1457 GAATGC GAATGC(1/-1)
BspDI 1565 ATCGAT AT^CGAT
BspHI 1365 TCATGA T^CATGA
BsrBI 1436 CCGCTC CCGCTC(-3/-3)
BsrDI 1235 GCAATG GCAATG(2/0)
Cac8I 90 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 1565 ATCGAT AT^CGAT
FauI 15 GCGGG CCCGC(4/6)
HgaI 242 GCGTC GACGC(5/10)
Hin1I 241 GGCGTC G[GA]^CG[CT]C
HincII 285 GTCAAC GT[CT]^[GA]AC
HindII 285 GTCAAC GT[CT]^[GA]AC
HindIII 746 AAGCTT A^AGCTT
HphI 1533 TCACC GGTGA(8/7)
MaeII 53 ACGT A^CGT
MspA1I 13 CAGCGG C[AC]G^C[GT]G
MwoI 638 GCTATAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 117 CCGGG CC^[GC]GG
PflFI 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 873 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 868 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1365 TCATGA T^CATGA
SexAI 146 ACCTGGT A^CC[AT]GGT
SwaI 723 ATTTAAAT ATTT^AAAT
Tth111I 279 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 873 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AciI 1436 CCGC CCGC(-3/-1)
15 GCGG
AfaI 114 GTAC GT^AC
650 GTAC
AflIII 652 ACATGT A^C[GA][CT]GT
1660 ACATGT
AlwI 211 GATCC GGATC(4/5)
368 GATCC
Asp700I 828 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1201 GAAAGAATTC
AspHI 61 GTGCTC G[AT]GC[AT]^C
264 GAGCAC
BalI 489 TGGCCA TGG^CCA
1593 TGGCCA
BfaI 253 CTAG C^TAG
1094 CTAG
BmyI 61 GTGCTC G[AGT]GC[ACT]^C
264 GAGCAC
BsiHKAI 61 GTGCTC G[AT]GC[AT]^C
264 GAGCAC
BsmAI 151 GTCTC GTCTC(1/5)
244 GTCTC
Bsp1286I 61 GTGCTC G[AGT]GC[ACT]^C
264 GAGCAC
BspLU11I 652 ACATGT A^CATGT
1660 ACATGT
BstYI 210 AGATCC [GA]^GATC[CT]
367 AGATCC
Cfr13I 26 GGCCC G^G[ACGT]CC
869 GGACC
DpnII 211 GATC ^GATC
368 GATC
DraII 25 AGGCCCC [GA]G^G[ACGT]CC[CT]
868 AGGACCC
EaeI 489 TGGCCA [CT]^GGCC[GA]
1593 TGGCCA
EarI 382 GAAGAG CTCTTC(1/4)
1804 GAAGAG
EcoO109I 25 AGGCCCC [GA]G^G[ACGT]CC[CT]
868 AGGACCC
EcoT22I 1459 ATGCAT ATGCA^T
1742 ATGCAT
HapII 117 CCGG C^CGG
1214 CCGG
HpaII 117 CCGG C^CGG
1214 CCGG
Ksp632I 382 GAAGAG CTCTTC(1/4)
1804 GAAGAG
MaeI 253 CTAG C^TAG
1094 CTAG
MboI 211 GATC ^GATC
368 GATC
MflI 210 AGATCC [GA]^GATC[CT]
367 AGATCC
MluNI 489 TGGCCA TGG^CCA
1593 TGGCCA
MscI 489 TGGCCA TGG^CCA
1593 TGGCCA
MspI 117 CCGG C^CGG
1214 CCGG
NdeII 211 GATC ^GATC
368 GATC
NsiI 1459 ATGCAT ATGCA^T
1742 ATGCAT
PleI 161 GACTC GAGTC(4/5)
1810 GACTC
RsaI 114 GTAC GT^AC
650 GTAC
Sau3AI 211 GATC ^GATC
368 GATC
Sau96I 26 GGCCC G^G[ACGT]CC
869 GGACC
SmlI 962 CTCAAG C^T[CT][GA]AG
1338 CTTAAG
TfiI 301 GATTC G^A[AT]TC
1626 GATTC
Tsp45I 36 GTCAC ^GT[GC]AC
1496 GTGAC
XhoII 210 AGATCC [GA]^GATC[CT]
367 AGATCC
XmnI 828 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1201 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI BbvI BpmI
BsaJI BsrI BssKI BstF5I BstNI
DdeI DraI EcoRI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboII MnlI MseI MslI MvaI
NlaIII NlaIV NspI ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I