Restriction map for
pF1KB5965
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspI AvrII BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraI
DraII DsaI EagI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 202 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 161 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 202 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1355 TGCGCA TGC^GCA
BalI 1718 TGGCCA TGG^CCA
BamHI 911 GGATCC G^GATCC
BanI 686 GGTGCC G^G[CT][GA]CC
BciVI 1010 GTATCC GTATCC(6/5)
BfaI 1252 CTAG C^TAG
BglI 1486 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 841 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 841 GCTAAGC GC^T[ACGT]AGC
Bsp1407I 1295 TGTACA T^GTACA
BsrDI 778 CATTGC GCAATG(2/0)
BsrGI 1295 TGTACA T^GTACA
Bst1107I 827 GTATAC GTA^TAC
BstZ17I 827 GTATAC GTA^TAC
CelII 841 GCTAAGC GC^T[ACGT]AGC
CfoI 1356 GCGC GCG^C
DraIII 1273 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 625 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1718 TGGCCA [CT]^GGCC[GA]
Eam1105I 202 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 513 CCTAACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1289 CCAAGG C^C[AT][AT]GG
FspI 1355 TGCGCA TGC^GCA
HgaI 244 GCGTC GACGC(5/10)
HhaI 1356 GCGC GCG^C
HinP1I 1356 GCGC G^CGC
MaeI 1252 CTAG C^TAG
MaeII 370 ACGT A^CGT
MluNI 1718 TGGCCA TGG^CCA
MscI 1718 TGGCCA TGG^CCA
NdeI 1351 CATATG CA^TATG
NspI 1284 GCATGC [GA]CATG^[CT]
SapI 448 GCTCTTC GCTCTTC(1/4)
SbfI 471 CCTGCAGG CCTGCA^GG
SmaI 572 CCCGGG CCC^GGG
SphI 1284 GCATGC GCATG^C
Sse8387I 471 CCTGCAGG CCTGCA^GG
SspBI 1295 TGTACA T^GTACA
StyI 1289 CCAAGG C^C[AT][AT]GG
Tsp509I 19 AATT ^AATT
XcmI 1581 CCAGGGTGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 572 CCCGGG C^CCGGG
XmaI 572 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 1124 GTGCAC G^TGCAC
1258 GTGCAC
ApaLI 1124 GTGCAC G^TGCAC
1258 GTGCAC
AvaII 270 GGTCC G^G[AT]CC
352 GGTCC
BpmI 1078 CTCCAG CTGGAG(16/14)
302 CTGGAG
BsaBI 655 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
726 GATGCAGATC
BsaI 754 GAGACC GGTCTC(1/5)
934 GAGACC
BsgI 1302 CTGCAC GTGCAG(16/14)
1390 GTGCAG
BspMI 1300 ACCTGC ACCTGC(4/8)
474 GCAGGT
BstAPI 1304 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1383 GCAACAGGTGC
BstXI 1560 CCAGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1700 CCATCCAGGTGG
BstYI 731 AGATCC [GA]^GATC[CT]
911 GGATCC
EarI 449 CTCTTC CTCTTC(1/4)
329 GAAGAG
FauI 957 CCCGC CCCGC(4/6)
1368 CCCGC
HphI 1105 GGTGA GGTGA(8/7)
1688 TCACC
Ksp632I 449 CTCTTC CTCTTC(1/4)
329 GAAGAG
MamI 655 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
726 GATGCAGATC
MflI 731 AGATCC [GA]^GATC[CT]
911 GGATCC
NlaIII 435 CATG CATG^
1285 CATG
PvuII 103 CAGCTG CAG^CTG
223 CAGCTG
StuI 1055 AGGCCT AGG^CCT
1484 AGGCCT
TaqI 68 TCGA T^CGA
508 TCGA
TthHB8I 68 TCGA T^CGA
508 TCGA
XhoII 731 AGATCC [GA]^GATC[CT]
911 GGATCC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AocI Asp700I AspHI AvaI BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII Eco81I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
TseI Tsp45I TspRI XmnI