################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 98 CCGC CCGC(-3/-1) AflIII 1321 ACATGT A^C[GA][CT]GT AlwI 315 GGATC GGATC(4/5) ApaI 107 GGGCCC GGGCC^C BbsI 556 GTCTTC GAAGAC(2/6) BclI 495 TGATCA T^GATCA BcnI 464 CCGGG CC^[GC]GG BglI 100 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 90 GCTGAGC GC^T[ACGT]AGC Bpu1102I 90 GCTGAGC GC^T[ACGT]AGC BpuAI 556 GTCTTC GAAGAC(2/6) BsaAI 777 TACGTA [CT]AC^GT[GA] BsaI 150 GGTCTC GGTCTC(1/5) BseRI 768 CTCCTC GAGGAG(10/8) BsgI 930 GTGCAG GTGCAG(16/14) BsmI 817 GAATGC GAATGC(1/-1) Bsp1407I 113 TGTACA T^GTACA BspLU11I 1321 ACATGT A^CATGT BspMI 14 ACCTGC ACCTGC(4/8) BsrGI 113 TGTACA T^GTACA BstEII 39 GGTGACC G^GT[ACGT]ACC BstPI 39 GGTGACC G^GT[ACGT]ACC CelII 90 GCTGAGC GC^T[ACGT]AGC DraI 898 TTTAAA TTT^AAA DsaI 23 CCATGG C^C[GA][CT]GG EcoO65I 39 GGTGACC G^GT[ACGT]ACC EcoT14I 23 CCATGG C^C[AT][AT]GG FauI 97 CCCGC CCCGC(4/6) FbaI 495 TGATCA T^GATCA HapII 464 CCGG C^CGG HpaII 464 CCGG C^CGG MspA1I 88 CAGCTG C[AC]G^C[GT]G MspI 464 CCGG C^CGG NciI 464 CCGGG CC^[GC]GG NcoI 23 CCATGG C^CATGG NdeI 1329 CATATG CA^TATG NheI 367 GCTAGC G^CTAGC NspI 1321 ACATGT [GA]CATG^[CT] PpuMI 168 AGGACCC [GA]G^G[AT]CC[CT] PvuII 88 CAGCTG CAG^CTG SexAI 146 ACCTGGT A^CC[AT]GGT SfcI 138 CTACAG C^T[GA][CT]AG SnaBI 777 TACGTA TAC^GTA SspBI 113 TGTACA T^GTACA StyI 23 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### Asp700I 1086 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1170 GAAAGCTTTC BbvI 87 GCAGC GCAGC(8/12) 411 GCAGC BsmAI 1545 GAGAC GTCTC(1/5) 151 GTCTC BsrDI 384 GCAATG GCAATG(2/0) 989 GCAATG Fnu4HI 87 GCAGC GC^[ACGT]GC 411 GCAGC ItaI 87 GCAGC GC^[ACGT]GC 411 GCAGC NlaIII 24 CATG CATG^ 1322 CATG PleI 161 GACTC GAGTC(4/5) 1449 GACTC SacI 477 GAGCTC GAGCT^C 765 GAGCTC StuI 752 AGGCCT AGG^CCT 1088 AGGCCT TseI 87 GCAGC G^C[AT]GC 411 GCAGC XmnI 1086 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1170 GAAAGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI AvaII BanII BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRI EcoRII FokI HaeIII HindIII HinfI HphI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I TspRI