##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
HphI        ItaI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    StuI        StyI        SwaI        TaqI        
TseI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   15 enzymes) #
###################################

Alw44I         16  GTGCAC               G^TGCAC
ApaLI          16  GTGCAC               G^TGCAC
Asp700I       153  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          16  GTGCAC               G[AT]GC[AT]^C
BsiHKAI        16  GTGCAC               G[AT]GC[AT]^C
BsiYI         269  CCTTTATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          269  CCTTTATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssKI         357  CCAGG                ^CC[ACGT]GG
BstNI         357  CCAGG                CC^[AT]GG
Cac8I         191  GCATGC               GC[ACGT]^[ACGT]GC
EcoRII        357  CCAGG                ^CC[AT]GG
MvaI          357  CCAGG                CC^[AT]GG
ScrFI         357  CCAGG                CC^[ACGT]GG
SphI          191  GCATGC               GCATG^C
XmnI          153  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI         1533  GCGG                 CCGC(-3/-1)
             1869  GCGG                
AfaI         1573  GTAC                 GT^AC
             1930  GTAC                
AvaII        1283  GGTCC                G^G[AT]CC
             1535  GGTCC               
BbsI          864  GTCTTC               GAAGAC(2/6)
             1872  GTCTTC              
BmyI           16  GTGCAC               G[AGT]GC[ACT]^C
             1552  GGGCAC              
BpuAI         864  GTCTTC               GAAGAC(2/6)
             1872  GTCTTC              
BsmAI        1826  GAGAC                GTCTC(1/5)
             1924  GAGAC               
Bsp1286I       16  GTGCAC               G[AGT]GC[ACT]^C
             1552  GGGCAC              
Bsp1407I     1572  TGTACA               T^GTACA
             1929  TGTACA              
BsrGI        1572  TGTACA               T^GTACA
             1929  TGTACA              
Bst1107I     1130  GTATAC               GTA^TAC
             1466  GTATAC              
BstZ17I      1130  GTATAC               GTA^TAC
             1466  GTATAC              
Cfr13I       1283  GGTCC                G^G[ACGT]CC
             1535  GGTCC               
MslI          661  CACAGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              829  CACAGATGTG          
NlaIII        192  CATG                 CATG^
             1850  CATG                
NspI          191  GCATGC               [GA]CATG^[CT]
             1849  GCATGT              
RsaI         1573  GTAC                 GT^AC
             1930  GTAC                
Sau96I       1283  GGTCC                G^G[ACGT]CC
             1535  GGTCC               
SspBI        1572  TGTACA               T^GTACA
             1929  TGTACA              
SspI           72  AATATT               AAT^ATT
              674  AATATT              
TfiI         1227  GATTC                G^A[AT]TC
             1703  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AccI        AcsI        AluI        ApoI        BfaI        
BpmI        BsrI        DdeI        DpnII       DraI        
EarI        EcoRI       HindIII     HinfI       Ksp632I     
MaeI        MboI        MboII       MnlI        MseI        
NdeII       PleI        Sau3AI      Tru9I       Tsp509I     
TspRI