################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BciVI BclI BcnI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AflII 316 CTTAAG C^TTAAG AluI 1080 AGCT AG^CT AlwNI 561 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 1511 GGTCC G^G[AT]CC BbvCI 196 CCTCAGC CCTCAGC(-5/-2) BfrI 316 CTTAAG C^TTAAG BglII 846 AGATCT A^GATCT BsaI 630 GAGACC GGTCTC(1/5) BsaJI 71 CCCAGG C^C[ACGT][ACGT]GG BsgI 1295 GTGCAG GTGCAG(16/14) BsiYI 1502 CCTTTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1502 CCTTTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 630 GAGAC GTCTC(1/5) BstXI 227 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 846 AGATCT [GA]^GATC[CT] Cac8I 1413 GCAAGC GC[ACGT]^[ACGT]GC DraI 1186 TTTAAA TTT^AAA EcoRV 421 GATATC GAT^ATC HindIII 1079 AAGCTT A^AGCTT MflI 846 AGATCT [GA]^GATC[CT] NdeI 1111 CATATG CA^TATG SfaNI 126 GATGC GCATC(5/9) XhoII 846 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 1259 GATCC GGATC(4/5) 93 GGATC AseI 175 ATTAAT AT^TAAT 1171 ATTAAT AsnI 175 ATTAAT AT^TAAT 1171 ATTAAT Bsp1407I 687 TGTACA T^GTACA 1107 TGTACA BsrGI 687 TGTACA T^GTACA 1107 TGTACA BssKI 72 CCAGG ^CC[ACGT]GG 1210 CCTGG BstNI 72 CCAGG CC^[AT]GG 1210 CCTGG Cfr13I 1472 GGCCC G^G[ACGT]CC 1511 GGTCC EarI 1092 CTCTTC CTCTTC(1/4) 1179 CTCTTC EcoRII 72 CCAGG ^CC[AT]GG 1210 CCTGG Ksp632I 1092 CTCTTC CTCTTC(1/4) 1179 CTCTTC MboII 1093 TCTTC GAAGA(8/7) 1180 TCTTC MvaI 72 CCAGG CC^[AT]GG 1210 CCTGG PshBI 175 ATTAAT AT^TAAT 1171 ATTAAT Sau96I 1472 GGCCC G^G[ACGT]CC 1511 GGTCC ScrFI 72 CCAGG CC^[ACGT]GG 1210 CCTGG SfcI 674 CTACAG C^T[GA][CT]AG 803 CTATAG SspBI 687 TGTACA T^GTACA 1107 TGTACA StuI 1303 AGGCCT AGG^CCT 1434 AGGCCT TfiI 144 GAATC G^A[AT]TC 1006 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AfaI ApoI Asp700I BfaI BpmI BseRI BsrI BstF5I DdeI DpnII FokI HaeIII HincII HindII HinfI HphI MaeI MaeIII MboI MnlI MseI MslI NdeII NlaIII NspI PleI RsaI Sau3AI SmlI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I XmnI