##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvI BcgI BciVI BclI
BcnI BglI BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII ItaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AflII 316 CTTAAG C^TTAAG
AluI 1080 AGCT AG^CT
AlwNI 561 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 1511 GGTCC G^G[AT]CC
BbvCI 196 CCTCAGC CCTCAGC(-5/-2)
BfrI 316 CTTAAG C^TTAAG
BglII 846 AGATCT A^GATCT
BsaI 630 GAGACC GGTCTC(1/5)
BsaJI 71 CCCAGG C^C[ACGT][ACGT]GG
BsgI 1295 GTGCAG GTGCAG(16/14)
BsiYI 1502 CCTTTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1502 CCTTTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 630 GAGAC GTCTC(1/5)
BstXI 227 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 846 AGATCT [GA]^GATC[CT]
Cac8I 1413 GCAAGC GC[ACGT]^[ACGT]GC
DraI 1186 TTTAAA TTT^AAA
EcoRV 421 GATATC GAT^ATC
HindIII 1079 AAGCTT A^AGCTT
MflI 846 AGATCT [GA]^GATC[CT]
NdeI 1111 CATATG CA^TATG
SfaNI 126 GATGC GCATC(5/9)
XhoII 846 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 1259 GATCC GGATC(4/5)
93 GGATC
AseI 175 ATTAAT AT^TAAT
1171 ATTAAT
AsnI 175 ATTAAT AT^TAAT
1171 ATTAAT
Bsp1407I 687 TGTACA T^GTACA
1107 TGTACA
BsrGI 687 TGTACA T^GTACA
1107 TGTACA
BssKI 72 CCAGG ^CC[ACGT]GG
1210 CCTGG
BstNI 72 CCAGG CC^[AT]GG
1210 CCTGG
Cfr13I 1472 GGCCC G^G[ACGT]CC
1511 GGTCC
EarI 1092 CTCTTC CTCTTC(1/4)
1179 CTCTTC
EcoRII 72 CCAGG ^CC[AT]GG
1210 CCTGG
Ksp632I 1092 CTCTTC CTCTTC(1/4)
1179 CTCTTC
MboII 1093 TCTTC GAAGA(8/7)
1180 TCTTC
MvaI 72 CCAGG CC^[AT]GG
1210 CCTGG
PshBI 175 ATTAAT AT^TAAT
1171 ATTAAT
Sau96I 1472 GGCCC G^G[ACGT]CC
1511 GGTCC
ScrFI 72 CCAGG CC^[ACGT]GG
1210 CCTGG
SfcI 674 CTACAG C^T[GA][CT]AG
803 CTATAG
SspBI 687 TGTACA T^GTACA
1107 TGTACA
StuI 1303 AGGCCT AGG^CCT
1434 AGGCCT
TfiI 144 GAATC G^A[AT]TC
1006 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI ApoI Asp700I BfaI
BpmI BseRI BsrI BstF5I DdeI
DpnII FokI HaeIII HincII HindII
HinfI HphI MaeI MaeIII MboI
MnlI MseI MslI NdeII NlaIII
NspI PleI RsaI Sau3AI SmlI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I XmnI