################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccII AciI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1119 GTCTAC GT^[AC][GT]AC AccIII 620 TCCGGA T^CCGGA AclI 1190 AACGTT AA^CGTT AlwI 1333 GATCC GGATC(4/5) AlwNI 1331 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 1434 GGCACC G^G[CT][GA]CC BglII 927 AGATCT A^GATCT BsaJI 47 CCCAGG C^C[ACGT][ACGT]GG BsaWI 620 TCCGGA [AT]^CCGG[AT] BseAI 620 TCCGGA T^CCGGA BseRI 460 GAGGAG GAGGAG(10/8) BsgI 989 CTGCAC GTGCAG(16/14) BsiYI 604 CCTGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 604 CCTGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 280 GAGAC GTCTC(1/5) BsmI 907 GAATGC GAATGC(1/-1) BspEI 620 TCCGGA T^CCGGA BspLU11I 1135 ACATGT A^CATGT BssKI 48 CCAGG ^CC[ACGT]GG BstNI 48 CCAGG CC^[AT]GG EarI 466 GAAGAG CTCTTC(1/4) EcoRII 48 CCAGG ^CC[AT]GG HapII 621 CCGG C^CGG HindIII 1218 AAGCTT A^AGCTT HpaII 621 CCGG C^CGG HphI 481 GGTGA GGTGA(8/7) Ksp632I 466 GAAGAG CTCTTC(1/4) MroI 620 TCCGGA T^CCGGA MspI 621 CCGG C^CGG MvaI 48 CCAGG CC^[AT]GG NdeI 1472 CATATG CA^TATG Psp1406I 1190 AACGTT AA^CGTT PstI 170 CTGCAG CTGCA^G ScrFI 48 CCAGG CC^[ACGT]GG SmlI 1675 CTTGAG C^T[CT][GA]AG SphI 42 GCATGC GCATG^C SwaI 680 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1135 ACATGT A^C[GA][CT]GT 1604 ACGTGT Asp700I 925 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1003 GAAACAATTC BclI 186 TGATCA T^GATCA 1593 TGATCA BsrDI 1508 CATTGC GCAATG(2/0) 658 GCAATG BstYI 927 AGATCT [GA]^GATC[CT] 1332 AGATCC Cac8I 42 GCATGC GC[ACGT]^[ACGT]GC 852 GCTTGC EcoRI 252 GAATTC G^AATTC 337 GAATTC FbaI 186 TGATCA T^GATCA 1593 TGATCA MflI 927 AGATCT [GA]^GATC[CT] 1332 AGATCC NlaIV 1378 GGTTCC GG[ACGT]^[ACGT]CC 1434 GGCACC SfaNI 1236 GATGC GCATC(5/9) 1582 GATGC SfcI 170 CTGCAG C^T[GA][CT]AG 1121 CTACAG XhoII 927 AGATCT [GA]^GATC[CT] 1332 AGATCC XmnI 925 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1003 GAAACAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsrI BstF5I DdeI DpnII DraI Fnu4HI FokI HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI TfiI Tru9I TseI Tsp45I Tsp509I TspRI