##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccII AciI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MslI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1119 GTCTAC GT^[AC][GT]AC
AccIII 620 TCCGGA T^CCGGA
AclI 1190 AACGTT AA^CGTT
AlwI 1333 GATCC GGATC(4/5)
AlwNI 1331 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 1434 GGCACC G^G[CT][GA]CC
BglII 927 AGATCT A^GATCT
BsaJI 47 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 620 TCCGGA [AT]^CCGG[AT]
BseAI 620 TCCGGA T^CCGGA
BseRI 460 GAGGAG GAGGAG(10/8)
BsgI 989 CTGCAC GTGCAG(16/14)
BsiYI 604 CCTGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 604 CCTGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 280 GAGAC GTCTC(1/5)
BsmI 907 GAATGC GAATGC(1/-1)
BspEI 620 TCCGGA T^CCGGA
BspLU11I 1135 ACATGT A^CATGT
BssKI 48 CCAGG ^CC[ACGT]GG
BstNI 48 CCAGG CC^[AT]GG
EarI 466 GAAGAG CTCTTC(1/4)
EcoRII 48 CCAGG ^CC[AT]GG
HapII 621 CCGG C^CGG
HindIII 1218 AAGCTT A^AGCTT
HpaII 621 CCGG C^CGG
HphI 481 GGTGA GGTGA(8/7)
Ksp632I 466 GAAGAG CTCTTC(1/4)
MroI 620 TCCGGA T^CCGGA
MspI 621 CCGG C^CGG
MvaI 48 CCAGG CC^[AT]GG
NdeI 1472 CATATG CA^TATG
Psp1406I 1190 AACGTT AA^CGTT
PstI 170 CTGCAG CTGCA^G
ScrFI 48 CCAGG CC^[ACGT]GG
SmlI 1675 CTTGAG C^T[CT][GA]AG
SphI 42 GCATGC GCATG^C
SwaI 680 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1135 ACATGT A^C[GA][CT]GT
1604 ACGTGT
Asp700I 925 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1003 GAAACAATTC
BclI 186 TGATCA T^GATCA
1593 TGATCA
BsrDI 1508 CATTGC GCAATG(2/0)
658 GCAATG
BstYI 927 AGATCT [GA]^GATC[CT]
1332 AGATCC
Cac8I 42 GCATGC GC[ACGT]^[ACGT]GC
852 GCTTGC
EcoRI 252 GAATTC G^AATTC
337 GAATTC
FbaI 186 TGATCA T^GATCA
1593 TGATCA
MflI 927 AGATCT [GA]^GATC[CT]
1332 AGATCC
NlaIV 1378 GGTTCC GG[ACGT]^[ACGT]CC
1434 GGCACC
SfaNI 1236 GATGC GCATC(5/9)
1582 GATGC
SfcI 170 CTGCAG C^T[GA][CT]AG
1121 CTACAG
XhoII 927 AGATCT [GA]^GATC[CT]
1332 AGATCC
XmnI 925 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1003 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsrI BstF5I DdeI DpnII
DraI Fnu4HI FokI HinfI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
NspI PleI RsaI Sau3AI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI