Restriction map for
pF1KB7873
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KasI
KpnI MaeI MamI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I Tth111I XbaI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 880 CGCG CG^CG
AcyI 518 GGCGTC G[GA]^CG[CT]C
AflIII 822 ACATGT A^C[GA][CT]GT
Alw44I 1012 GTGCAC G^TGCAC
AlwI 99 GATCC GGATC(4/5)
AocI 713 CCTCAGG CC^T[ACGT]AGG
ApaI 1286 GGGCCC GGGCC^C
ApaLI 1012 GTGCAC G^TGCAC
Asp700I 969 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1345 GAAGAC GAAGAC(2/6)
BbvCI 1525 GCTGAGG CCTCAGC(-5/-2)
BclI 1304 TGATCA T^GATCA
BpuAI 1345 GAAGAC GAAGAC(2/6)
BsaHI 518 GGCGTC G[GA]^CG[CT]C
BsaI 719 GAGACC GGTCTC(1/5)
BsmBI 520 CGTCTC CGTCTC(1/5)
BsmI 490 GCATTC GAATGC(1/-1)
BspHI 438 TCATGA T^CATGA
BspLU11I 822 ACATGT A^CATGT
BsrDI 1596 CATTGC GCAATG(2/0)
BsrFI 1061 ACCGGC [GA]^CCGG[CT]
BstAPI 1040 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 880 CGCG CG^CG
Bsu36I 713 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1061 ACCGGC [GA]^CCGG[CT]
DrdI 1686 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 713 CCTCAGG CC^T[ACGT]AGG
EcoNI 1218 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 965 GCGGG CCCGC(4/6)
FbaI 1304 TGATCA T^GATCA
Hin1I 518 GGCGTC G[GA]^CG[CT]C
HindIII 79 AAGCTT A^AGCTT
KspI 879 CCGCGG CCGC^GG
MseI 544 TTAA T^TAA
MvnI 880 CGCG CG^CG
PflMI 297 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 359 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 438 TCATGA T^CATGA
SacI 560 GAGCTC GAGCT^C
SacII 879 CCGCGG CCGC^GG
SapI 636 GAAGAGC GCTCTTC(1/4)
SbfI 1350 CCTGCAGG CCTGCA^GG
ScaI 983 AGTACT AGT^ACT
SexAI 134 ACCAGGT A^CC[AT]GGT
SfiI 1647 GGCCACTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1350 CCTGCAGG CCTGCA^GG
Tru9I 544 TTAA T^TAA
Van91I 297 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 969 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1041 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1354 CAGGCCCTG
AvaI 106 CTCGAG C^[CT]CG[GA]G
987 CTCGAG
AvaII 116 GGACC G^G[AT]CC
360 GGACC
AviII 827 TGCGCA TGC^GCA
917 TGCGCA
BbrPI 913 CACGTG CAC^GTG
1006 CACGTG
BsaAI 913 CACGTG [CT]AC^GT[GA]
1006 CACGTG
BsoBI 106 CTCGAG C^[CT]CG[GA]G
987 CTCGAG
BsrBI 533 CCGCTC CCGCTC(-3/-3)
789 CCGCTC
DpnII 99 GATC ^GATC
1305 GATC
FspI 827 TGCGCA TGC^GCA
917 TGCGCA
HaeII 892 AGCGCC [GA]GCGC^[CT]
1468 AGCGCC
MboI 99 GATC ^GATC
1305 GATC
NcoI 1101 CCATGG C^CATGG
1460 CCATGG
NdeII 99 GATC ^GATC
1305 GATC
NspI 732 GCATGT [GA]CATG^[CT]
822 ACATGT
PaeR7I 106 CTCGAG C^TCGAG
987 CTCGAG
PleI 1531 GACTC GAGTC(4/5)
1185 GAGTC
PmaCI 913 CACGTG CAC^GTG
1006 CACGTG
PmlI 913 CACGTG CAC^GTG
1006 CACGTG
Sau3AI 99 GATC ^GATC
1305 GATC
StuI 611 AGGCCT AGG^CCT
701 AGGCCT
TaqI 107 TCGA T^CGA
988 TCGA
TfiI 450 GATTC G^A[AT]TC
909 GAATC
TthHB8I 107 TCGA T^CGA
988 TCGA
XcmI 102 CCAGCTCGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
264 CCAACTGGAAGATGG
XhoI 106 CTCGAG C^TCGAG
987 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI Cac8I
CfoI Cfr13I DdeI DraII DraIII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I TspRI