################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 428 AAATTC [GA]^AATT[CT] AflIII 147 ACATGT A^C[GA][CT]GT AlwNI 51 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 428 AAATTC [GA]^AATT[CT] Asp700I 370 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 66 GTGCTC G[AT]GC[AT]^C BbsI 473 GAAGAC GAAGAC(2/6) BbvCI 214 GCTGAGG CCTCAGC(-5/-2) BbvI 450 GCAGC GCAGC(8/12) BmyI 66 GTGCTC G[AGT]GC[ACT]^C BpmI 57 CTGGAG CTGGAG(16/14) BpuAI 473 GAAGAC GAAGAC(2/6) BseRI 241 GAGGAG GAGGAG(10/8) BsiHKAI 66 GTGCTC G[AT]GC[AT]^C BsiYI 210 CCCAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 210 CCCAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 66 GTGCTC G[AGT]GC[ACT]^C BspHI 234 TCATGA T^CATGA BspLU11I 147 ACATGT A^CATGT BsrDI 298 GCAATG GCAATG(2/0) Cac8I 32 GCATGC GC[ACGT]^[ACGT]GC Fnu4HI 450 GCAGC GC^[ACGT]GC HincII 303 GTTGAC GT[CT]^[GA]AC HindII 303 GTTGAC GT[CT]^[GA]AC ItaI 450 GCAGC GC^[ACGT]GC MaeIII 332 GTCAC ^GT[ACGT]AC MseI 361 TTAA T^TAA MwoI 94 GCATTAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC RcaI 234 TCATGA T^CATGA SphI 32 GCATGC GCATG^C Tru9I 361 TTAA T^TAA TseI 450 GCAGC G^C[AT]GC Tsp45I 332 GTCAC ^GT[GC]AC TspRI 352 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 370 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsrI 353 ACTGG ACTGG(1/-1) 385 ACTGG DdeI 69 CTCAG C^T[ACGT]AG 215 CTGAG EarI 79 GAAGAG CTCTTC(1/4) 238 GAAGAG EcoT22I 30 ATGCAT ATGCA^T 121 ATGCAT Ksp632I 79 GAAGAG CTCTTC(1/4) 238 GAAGAG MspA1I 212 CAGCTG C[AC]G^C[GT]G 451 CAGCTG NsiI 30 ATGCAT ATGCA^T 121 ATGCAT NspI 32 GCATGC [GA]CATG^[CT] 147 ACATGT PleI 168 GACTC GAGTC(4/5) 60 GAGTC PvuII 212 CAGCTG CAG^CTG 451 CAGCTG TfiI 73 GATTC G^A[AT]TC 496 GATTC Tsp509I 199 AATT ^AATT 429 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AluI BssKI BstNI EcoRII HinfI MboII MnlI MvaI NlaIII ScrFI