##################################
# NO CUT ENZYMES ( 201 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FokI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
HphI KasI KpnI KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 428 AAATTC [GA]^AATT[CT]
AflIII 147 ACATGT A^C[GA][CT]GT
AlwNI 51 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 428 AAATTC [GA]^AATT[CT]
Asp700I 370 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 66 GTGCTC G[AT]GC[AT]^C
BbsI 473 GAAGAC GAAGAC(2/6)
BbvCI 214 GCTGAGG CCTCAGC(-5/-2)
BbvI 450 GCAGC GCAGC(8/12)
BmyI 66 GTGCTC G[AGT]GC[ACT]^C
BpmI 57 CTGGAG CTGGAG(16/14)
BpuAI 473 GAAGAC GAAGAC(2/6)
BseRI 241 GAGGAG GAGGAG(10/8)
BsiHKAI 66 GTGCTC G[AT]GC[AT]^C
BsiYI 210 CCCAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 210 CCCAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 66 GTGCTC G[AGT]GC[ACT]^C
BspHI 234 TCATGA T^CATGA
BspLU11I 147 ACATGT A^CATGT
BsrDI 298 GCAATG GCAATG(2/0)
Cac8I 32 GCATGC GC[ACGT]^[ACGT]GC
Fnu4HI 450 GCAGC GC^[ACGT]GC
HincII 303 GTTGAC GT[CT]^[GA]AC
HindII 303 GTTGAC GT[CT]^[GA]AC
ItaI 450 GCAGC GC^[ACGT]GC
MaeIII 332 GTCAC ^GT[ACGT]AC
MseI 361 TTAA T^TAA
MwoI 94 GCATTAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI 234 TCATGA T^CATGA
SphI 32 GCATGC GCATG^C
Tru9I 361 TTAA T^TAA
TseI 450 GCAGC G^C[AT]GC
Tsp45I 332 GTCAC ^GT[GC]AC
TspRI 352 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 370 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsrI 353 ACTGG ACTGG(1/-1)
385 ACTGG
DdeI 69 CTCAG C^T[ACGT]AG
215 CTGAG
EarI 79 GAAGAG CTCTTC(1/4)
238 GAAGAG
EcoT22I 30 ATGCAT ATGCA^T
121 ATGCAT
Ksp632I 79 GAAGAG CTCTTC(1/4)
238 GAAGAG
MspA1I 212 CAGCTG C[AC]G^C[GT]G
451 CAGCTG
NsiI 30 ATGCAT ATGCA^T
121 ATGCAT
NspI 32 GCATGC [GA]CATG^[CT]
147 ACATGT
PleI 168 GACTC GAGTC(4/5)
60 GAGTC
PvuII 212 CAGCTG CAG^CTG
451 CAGCTG
TfiI 73 GATTC G^A[AT]TC
496 GATTC
Tsp509I 199 AATT ^AATT
429 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BssKI BstNI EcoRII HinfI
MboII MnlI MvaI NlaIII ScrFI