Restriction map for
pF1KE0554
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TfiI Tru9I Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 474 ACGTGT A^C[GA][CT]GT
AlwI 201 GATCC GGATC(4/5)
ApaI 224 GGGCCC GGGCC^C
AspHI 563 GAGCTC G[AT]GC[AT]^C
BalI 17 TGGCCA TGG^CCA
BbsI 361 GAAGAC GAAGAC(2/6)
BciVI 706 GTATCC GTATCC(6/5)
BfaI 191 CTAG C^TAG
BpuAI 361 GAAGAC GAAGAC(2/6)
BsgI 320 CTGCAC GTGCAG(16/14)
BsiEI 335 CGGTCG CG[GA][CT]^CG
BsiHKAI 563 GAGCTC G[AT]GC[AT]^C
BsmI 217 GAATGC GAATGC(1/-1)
Bsp1407I 477 TGTACA T^GTACA
BspHI 213 TCATGA T^CATGA
BsrBI 664 GAGCGG CCGCTC(-3/-3)
BsrDI 486 CATTGC GCAATG(2/0)
BsrFI 333 GCCGGT [GA]^CCGG[CT]
BsrGI 477 TGTACA T^GTACA
BstYI 200 AGATCC [GA]^GATC[CT]
Cfr10I 333 GCCGGT [GA]^CCGG[CT]
EaeI 17 TGGCCA [CT]^GGCC[GA]
HaeII 63 AGCGCC [GA]GCGC^[CT]
MaeI 191 CTAG C^TAG
MaeII 474 ACGT A^CGT
MflI 200 AGATCC [GA]^GATC[CT]
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
PflMI 380 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 154 GGGACCC [GA]G^G[AT]CC[CT]
PstI 511 CTGCAG CTGCA^G
RcaI 213 TCATGA T^CATGA
SacI 563 GAGCTC GAGCT^C
SfcI 511 CTGCAG C^T[GA][CT]AG
SspBI 477 TGTACA T^GTACA
StuI 262 AGGCCT AGG^CCT
Tsp509I 538 AATT ^AATT
Van91I 380 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 200 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 478 GTAC GT^AC
594 GTAC
AocI 528 CCTGAGG CC^T[ACGT]AGG
658 CCTCAGG
AvaI 50 CCCGGG C^[CT]CG[GA]G
341 CCCGGG
AvaII 155 GGACC G^G[AT]CC
393 GGTCC
BanII 224 GGGCCC G[GA]GC[CT]^C
563 GAGCTC
BglI 66 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
673 GCCTCCAAGGC
BmyI 224 GGGCCC G[AGT]GC[ACT]^C
563 GAGCTC
BseRI 418 GAGGAG GAGGAG(10/8)
714 GAGGAG
BsoBI 50 CCCGGG C^[CT]CG[GA]G
341 CCCGGG
Bsp1286I 224 GGGCCC G[AGT]GC[ACT]^C
563 GAGCTC
BspMI 248 ACCTGC ACCTGC(4/8)
106 GCAGGT
Bsu36I 528 CCTGAGG CC^T[ACGT]AGG
658 CCTCAGG
DpnII 201 GATC ^GATC
641 GATC
Eco81I 528 CCTGAGG CC^T[ACGT]AGG
658 CCTCAGG
EcoT14I 228 CCAAGG C^C[AT][AT]GG
677 CCAAGG
FauI 221 GCGGG CCCGC(4/6)
666 GCGGG
HphI 415 GGTGA GGTGA(8/7)
293 TCACC
MboI 201 GATC ^GATC
641 GATC
MboII 358 GAAGA GAAGA(8/7)
361 GAAGA
MspA1I 369 CCGCTG C[AC]G^C[GT]G
411 CAGCGG
NdeII 201 GATC ^GATC
641 GATC
NlaIII 214 CATG CATG^
481 CATG
RsaI 478 GTAC GT^AC
594 GTAC
Sau3AI 201 GATC ^GATC
641 GATC
SmaI 50 CCCGGG CCC^GGG
341 CCCGGG
StyI 228 CCAAGG C^C[AT][AT]GG
677 CCAAGG
XmaCI 50 CCCGGG C^CCGGG
341 CCCGGG
XmaI 50 CCCGGG C^CCGGG
341 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwNI BbvI BcnI
BpmI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MslI MspI MvaI MwoI NciI
NlaIV Sau96I ScrFI SfaNI TseI
TspRI