Restriction map for
pF1KB6855
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstXI BstZ17I Cfr10I CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AocI 354 CCTCAGG CC^T[ACGT]AGG
Asp700I 153 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 30 GGTCC G^G[AT]CC
BanI 67 GGCACC G^G[CT][GA]CC
BbvCI 360 GCTGAGG CCTCAGC(-5/-2)
BbvI 86 GCTGC GCAGC(8/12)
BglII 126 AGATCT A^GATCT
BlpI 8 GCTCAGC GC^T[ACGT]AGC
BmyI 66 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 8 GCTCAGC GC^T[ACGT]AGC
BseRI 274 GAGGAG GAGGAG(10/8)
BsgI 87 CTGCAC GTGCAG(16/14)
BsmI 429 GAATGC GAATGC(1/-1)
Bsp1286I 66 GGGCAC G[AGT]GC[ACT]^C
BspDI 380 ATCGAT AT^CGAT
BstEII 226 GGTTACC G^GT[ACGT]ACC
BstPI 226 GGTTACC G^GT[ACGT]ACC
BstYI 126 AGATCT [GA]^GATC[CT]
Bsu36I 354 CCTCAGG CC^T[ACGT]AGG
Cac8I 52 GCCAGC GC[ACGT]^[ACGT]GC
CelII 8 GCTCAGC GC^T[ACGT]AGC
ClaI 380 ATCGAT AT^CGAT
DsaI 413 CCGTGG C^C[GA][CT]GG
Eco81I 354 CCTCAGG CC^T[ACGT]AGG
EcoNI 270 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 226 GGTTACC G^GT[ACGT]ACC
Fnu4HI 86 GCTGC GC^[ACGT]GC
HaeII 371 GGCGCT [GA]GCGC^[CT]
HinfI 202 GAGTC G^A[ACGT]TC
ItaI 86 GCTGC GC^[ACGT]GC
MaeIII 227 GTTAC ^GT[ACGT]AC
MflI 126 AGATCT [GA]^GATC[CT]
MslI 513 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 111 GCCTAACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 67 GGCACC GG[ACGT]^[ACGT]CC
PleI 202 GAGTC GAGTC(4/5)
TseI 86 GCTGC G^C[AT]GC
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 126 AGATCT [GA]^GATC[CT]
XmnI 153 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 258 GATCC GGATC(4/5)
174 GGATC
BbsI 153 GAAGAC GAAGAC(2/6)
348 GAAGAC
BpmI 211 CTGGAG CTGGAG(16/14)
271 CTGGAG
BpuAI 153 GAAGAC GAAGAC(2/6)
348 GAAGAC
BspMI 107 ACCTGC ACCTGC(4/8)
420 GCAGGT
CfoI 319 GCGC GCG^C
372 GCGC
Cfr13I 30 GGTCC G^G[ACGT]CC
50 GGGCC
HhaI 319 GCGC GCG^C
372 GCGC
HinP1I 319 GCGC G^CGC
372 GCGC
MspA1I 84 CAGCTG C[AC]G^C[GT]G
178 CAGCTG
NspI 116 ACATGC [GA]CATG^[CT]
321 GCATGT
PvuII 84 CAGCTG CAG^CTG
178 CAGCTG
Sau96I 30 GGTCC G^G[ACGT]CC
50 GGGCC
TaqI 123 TCGA T^CGA
381 TCGA
TthHB8I 123 TCGA T^CGA
381 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsrI
BssKI BstNI DdeI DpnII EcoRII
HaeIII HphI MboI MboII MnlI
MseI MvaI NdeII NlaIII Sau3AI
ScrFI SfaNI Tru9I