##################################
# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NlaIV NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 88 GCGG CCGC(-3/-1)
AflII 316 CTTAAG C^TTAAG
AlwI 312 GGATC GGATC(4/5)
Asp700I 51 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 501 GAGCCC G[GA]GC[CT]^C
BbsI 606 GAAGAC GAAGAC(2/6)
BbvI 390 GCTGC GCAGC(8/12)
BfrI 316 CTTAAG C^TTAAG
BpuAI 606 GAAGAC GAAGAC(2/6)
BsaJI 659 CCACGG C^C[ACGT][ACGT]GG
BsiYI 635 CCAATGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 635 CCAATGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 369 GAATGC GAATGC(1/-1)
BsrI 15 ACTGG ACTGG(1/-1)
BssKI 309 CCAGG ^CC[ACGT]GG
BstBI 437 TTCGAA TT^CGAA
BstNI 309 CCAGG CC^[AT]GG
BstYI 312 GGATCT [GA]^GATC[CT]
Cac8I 206 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 576 GGGCC G^G[ACGT]CC
DraII 575 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 659 CCACGG C^C[GA][CT]GG
EcoO109I 575 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 309 CCAGG ^CC[AT]GG
FauI 88 GCGGG CCCGC(4/6)
Fnu4HI 390 GCTGC GC^[ACGT]GC
HaeIII 577 GGCC GG^CC
HindIII 647 AAGCTT A^AGCTT
HphI 451 GGTGA GGTGA(8/7)
ItaI 390 GCTGC GC^[ACGT]GC
MflI 312 GGATCT [GA]^GATC[CT]
MslI 523 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 309 CCAGG CC^[AT]GG
NspV 437 TTCGAA TT^CGAA
Sau96I 576 GGGCC G^G[ACGT]CC
ScaI 291 AGTACT AGT^ACT
ScrFI 309 CCAGG CC^[ACGT]GG
SfuI 437 TTCGAA TT^CGAA
TseI 390 GCTGC G^C[AT]GC
XhoII 312 GGATCT [GA]^GATC[CT]
XmnI 51 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 65 GTAC GT^AC
292 GTAC
BmyI 501 GAGCCC G[AGT]GC[ACT]^C
560 GTGCCC
BsmFI 90 GGGAC GGGAC(10/14)
539 GGGAC
Bsp1286I 501 GAGCCC G[AGT]GC[ACT]^C
560 GTGCCC
HinfI 112 GATTC G^A[ACGT]TC
492 GAATC
PstI 391 CTGCAG CTGCA^G
401 CTGCAG
RsaI 65 GTAC GT^AC
292 GTAC
SfcI 391 CTGCAG C^T[GA][CT]AG
401 CTGCAG
SmlI 316 CTTAAG C^T[CT][GA]AG
571 CTTGAG
SspI 326 AATATT AAT^ATT
478 AATATT
TfiI 112 GATTC G^A[AT]TC
492 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AluI ApoI BfaI DdeI
DpnII MaeI MboI MboII MnlI
MseI MwoI NdeII Sau3AI TaqI
Tru9I Tsp509I TthHB8I