################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 88 GCGG CCGC(-3/-1) AflII 316 CTTAAG C^TTAAG AlwI 312 GGATC GGATC(4/5) Asp700I 51 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 501 GAGCCC G[GA]GC[CT]^C BbsI 606 GAAGAC GAAGAC(2/6) BbvI 390 GCTGC GCAGC(8/12) BfrI 316 CTTAAG C^TTAAG BpuAI 606 GAAGAC GAAGAC(2/6) BsaJI 659 CCACGG C^C[ACGT][ACGT]GG BsiYI 635 CCAATGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 635 CCAATGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 369 GAATGC GAATGC(1/-1) BsrI 15 ACTGG ACTGG(1/-1) BssKI 309 CCAGG ^CC[ACGT]GG BstBI 437 TTCGAA TT^CGAA BstNI 309 CCAGG CC^[AT]GG BstYI 312 GGATCT [GA]^GATC[CT] Cac8I 206 GCAGGC GC[ACGT]^[ACGT]GC Cfr13I 576 GGGCC G^G[ACGT]CC DraII 575 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 659 CCACGG C^C[GA][CT]GG EcoO109I 575 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRII 309 CCAGG ^CC[AT]GG FauI 88 GCGGG CCCGC(4/6) Fnu4HI 390 GCTGC GC^[ACGT]GC HaeIII 577 GGCC GG^CC HindIII 647 AAGCTT A^AGCTT HphI 451 GGTGA GGTGA(8/7) ItaI 390 GCTGC GC^[ACGT]GC MflI 312 GGATCT [GA]^GATC[CT] MslI 523 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 309 CCAGG CC^[AT]GG NspV 437 TTCGAA TT^CGAA Sau96I 576 GGGCC G^G[ACGT]CC ScaI 291 AGTACT AGT^ACT ScrFI 309 CCAGG CC^[ACGT]GG SfuI 437 TTCGAA TT^CGAA TseI 390 GCTGC G^C[AT]GC XhoII 312 GGATCT [GA]^GATC[CT] XmnI 51 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 65 GTAC GT^AC 292 GTAC BmyI 501 GAGCCC G[AGT]GC[ACT]^C 560 GTGCCC BsmFI 90 GGGAC GGGAC(10/14) 539 GGGAC Bsp1286I 501 GAGCCC G[AGT]GC[ACT]^C 560 GTGCCC HinfI 112 GATTC G^A[ACGT]TC 492 GAATC PstI 391 CTGCAG CTGCA^G 401 CTGCAG RsaI 65 GTAC GT^AC 292 GTAC SfcI 391 CTGCAG C^T[GA][CT]AG 401 CTGCAG SmlI 316 CTTAAG C^T[CT][GA]AG 571 CTTGAG SspI 326 AATATT AAT^ATT 478 AATATT TfiI 112 GATTC G^A[AT]TC 492 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AcsI AluI ApoI BfaI DdeI DpnII MaeI MboI MboII MnlI MseI MwoI NdeII Sau3AI TaqI Tru9I Tsp509I TthHB8I