Restriction map for
pF1KB0026
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AccI AccIII AclI AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BciVI BfaI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 2659 GACGTC GACGT^C
AccII 1048 CGCG CG^CG
AflII 718 CTTAAG C^TTAAG
AocI 991 CCTCAGG CC^T[ACGT]AGG
Aor51HI 765 AGCGCT AGC^GCT
ApaI 1838 GGGCCC GGGCC^C
Asp700I 1623 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1703 GGATCC G^GATCC
BfrI 718 CTTAAG C^TTAAG
BglII 1163 AGATCT A^GATCT
BsiEI 2426 CGGCCG CG[GA][CT]^CG
BsmBI 1633 CGTCTC CGTCTC(1/5)
BsmI 235 GCATTC GAATGC(1/-1)
Bsp1407I 2432 TGTACA T^GTACA
BspDI 79 ATCGAT AT^CGAT
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrDI 330 CATTGC GCAATG(2/0)
BsrGI 2432 TGTACA T^GTACA
BstUI 1048 CGCG CG^CG
Bsu36I 991 CCTCAGG CC^T[ACGT]AGG
ClaI 79 ATCGAT AT^CGAT
DraIII 3162 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2426 CGGCCG C^GGCCG
EclXI 2426 CGGCCG C^GGCCG
Eco47III 765 AGCGCT AGC^GCT
Eco52I 2426 CGGCCG C^GGCCG
Eco81I 991 CCTCAGG CC^T[ACGT]AGG
EcoRV 628 GATATC GAT^ATC
MseI 719 TTAA T^TAA
MvnI 1048 CGCG CG^CG
NcoI 488 CCATGG C^CATGG
PflMI 2415 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 1956 CCTGCAGG CCTGCA^GG
ScaI 2051 AGTACT AGT^ACT
SexAI 1389 ACCAGGT A^CC[AT]GGT
Sse8387I 1956 CCTGCAGG CCTGCA^GG
SspBI 2432 TGTACA T^GTACA
Tru9I 719 TTAA T^TAA
Van91I 2415 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1623 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 730 GTGCAC G^TGCAC
2281 GTGCAC
ApaLI 730 GTGCAC G^TGCAC
2281 GTGCAC
AspI 19 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAAAGTC
BbeI 1472 GGCGCC GGCGC^C
2010 GGCGCC
BbsI 1446 GAAGAC GAAGAC(2/6)
2761 GTCTTC
BglI 1885 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2771 GCCTGCTGGGC
BpuAI 1446 GAAGAC GAAGAC(2/6)
2761 GTCTTC
BsaI 798 GAGACC GGTCTC(1/5)
2099 GAGACC
BsgI 907 CTGCAC GTGCAG(16/14)
2578 CTGCAC
BssSI 2568 CACGAG CACGAG(-5/-1)
650 CTCGTG
BstAPI 1206 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2048 GCAAGTACTGC
DsaI 488 CCATGG C^C[GA][CT]GG
1307 CCGTGG
KasI 1472 GGCGCC G^GCGCC
2010 GGCGCC
NarI 1472 GGCGCC GG^CGCC
2010 GGCGCC
PflFI 19 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAAAGTC
PshAI 2559 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2947 GACCTCAGTC
SfoI 1472 GGCGCC GGC^GCC
2010 GGCGCC
SphI 295 GCATGC GCATG^C
843 GCATGC
TfiI 897 GAATC G^A[AT]TC
2975 GAATC
Tth111I 19 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2593 GACAAAGTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
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AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcgI BclI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII