Restriction map for
pF1KE1066
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I AlwNI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BalI BbeI BbrPI BbvCI BciVI
BclI BglI BglII BlpI Bpu1102I
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI EarI EcoNI EcoRI
EcoT22I FbaI FseI FspI HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MslI
MunI NarI NdeI NheI NotI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 193 GTCTAC GT^[AC][GT]AC
AhdI 743 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1122 CCTCAGG CC^T[ACGT]AGG
Aor51HI 122 AGCGCT AGC^GCT
AspEI 743 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 72 GGACC G^G[AT]CC
AvrII 1164 CCTAGG C^CTAGG
BamHI 1224 GGATCC G^GATCC
BanI 470 GGCACC G^G[CT][GA]CC
BbsI 663 GTCTTC GAAGAC(2/6)
BcgI 1429 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1164 CCTAGG C^CTAGG
BpuAI 663 GTCTTC GAAGAC(2/6)
BsaAI 109 TACGTG [CT]AC^GT[GA]
BsaBI 964 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 647 GAGACC GGTCTC(1/5)
BsgI 1406 GTGCAG GTGCAG(16/14)
BsmBI 5 CGTCTC CGTCTC(1/5)
BsmI 1053 GAATGC GAATGC(1/-1)
BsrFI 149 GCCGGC [GA]^CCGG[CT]
BstF5I 1415 GGATG GGATG(2/0)
BstYI 1224 GGATCC [GA]^GATC[CT]
Bsu36I 1122 CCTCAGG CC^T[ACGT]AGG
Cfr10I 149 GCCGGC [GA]^CCGG[CT]
DraI 1453 TTTAAA TTT^AAA
Eam1105I 743 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 122 AGCGCT AGC^GCT
Eco81I 1122 CCTCAGG CC^T[ACGT]AGG
EcoRV 1333 GATATC GAT^ATC
FokI 1415 GGATG GGATG(9/13)
HincII 940 GTCAAC GT[CT]^[GA]AC
HindII 940 GTCAAC GT[CT]^[GA]AC
MaeII 110 ACGT A^CGT
MamI 964 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1224 GGATCC [GA]^GATC[CT]
NaeI 149 GCCGGC GCC^GGC
NcoI 1211 CCATGG C^CATGG
NgoMIV 149 GCCGGC G^CCGGC
NruI 1394 TCGCGA TCG^CGA
NspI 1086 GCATGT [GA]CATG^[CT]
PaeR7I 1034 CTCGAG C^TCGAG
PflMI 1344 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 71 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1427 CTGCAG CTGCA^G
SacI 598 GAGCTC GAGCT^C
SfaNI 94 GATGC GCATC(5/9)
SgrAI 148 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1060 AATATT AAT^ATT
StuI 417 AGGCCT AGG^CCT
Van91I 1344 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1211 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1034 CTCGAG C^TCGAG
XhoII 1224 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
1380 GGCGTC
AflII 253 CTTAAG C^TTAAG
546 CTTAAG
ApaI 52 GGGCCC GGGCC^C
1177 GGGCCC
Asp700I 268 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
415 GAAGGCCTTC
BfaI 1165 CTAG C^TAG
1316 CTAG
BfrI 253 CTTAAG C^TTAAG
546 CTTAAG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
1380 GGCGTC
BstEII 517 GGTGACC G^GT[ACGT]ACC
1440 GGTGACC
BstPI 517 GGTGACC G^GT[ACGT]ACC
1440 GGTGACC
DsaI 86 CCGTGG C^C[GA][CT]GG
1211 CCATGG
EaeI 58 CGGCCG [CT]^GGCC[GA]
204 CGGCCG
EagI 58 CGGCCG C^GGCCG
204 CGGCCG
EclXI 58 CGGCCG C^GGCCG
204 CGGCCG
Eco52I 58 CGGCCG C^GGCCG
204 CGGCCG
EcoO65I 517 GGTGACC G^GT[ACGT]ACC
1440 GGTGACC
HaeII 30 GGCGCT [GA]GCGC^[CT]
122 AGCGCT
Hin1I 3 GGCGTC G[GA]^CG[CT]C
1380 GGCGTC
MaeI 1165 CTAG C^TAG
1316 CTAG
PvuII 840 CAGCTG CAG^CTG
934 CAGCTG
SfcI 837 CTACAG C^T[GA][CT]AG
1427 CTGCAG
SmaI 26 CCCGGG CCC^GGG
68 CCCGGG
XmaCI 26 CCCGGG C^CCGGG
68 CCCGGG
XmaI 26 CCCGGG C^CCGGG
68 CCCGGG
XmnI 268 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
415 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SexAI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I