Restriction map for
pF1KB8595
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccI AccII AccIII AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcnI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HaeII
HapII HgaI HhaI HinP1I HpaI
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 1983 ACATGT A^C[GA][CT]GT
AhdI 3506 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1105 CCTAAGG CC^T[ACGT]AGG
ApaI 5949 GGGCCC GGGCC^C
AspEI 3506 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2226 TGGCCA TGG^CCA
BanII 5949 GGGCCC G[GA]GC[CT]^C
BbvCI 3835 GCTGAGG CCTCAGC(-5/-2)
BcgI 1542 GCATGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1118 GGATAC GTATCC(6/5)
BglI 4729 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 735 AGATCT A^GATCT
BsaAI 4154 TACGTG [CT]AC^GT[GA]
BsaBI 5844 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 13 CGTCTC CGTCTC(1/5)
BspLU11I 1983 ACATGT A^CATGT
BspMI 1899 GCAGGT ACCTGC(4/8)
BssSI 6132 CTCGTG CACGAG(-5/-1)
BstBI 6407 TTCGAA TT^CGAA
Bsu36I 1105 CCTAAGG CC^T[ACGT]AGG
DraI 1411 TTTAAA TTT^AAA
DraIII 2146 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4264 GACCTTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 6035 CCATGG C^C[GA][CT]GG
Eam1105I 3506 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1105 CCTAAGG CC^T[ACGT]AGG
EcoNI 5679 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3028 GATATC GAT^ATC
FauI 2455 CCCGC CCCGC(4/6)
MamI 5844 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2226 TGGCCA TGG^CCA
MscI 2226 TGGCCA TGG^CCA
NcoI 6035 CCATGG C^CATGG
NheI 3370 GCTAGC G^CTAGC
NspV 6407 TTCGAA TT^CGAA
PpuMI 816 GGGACCT [GA]G^G[AT]CC[CT]
SapI 913 GCTCTTC GCTCTTC(1/4)
ScaI 5521 AGTACT AGT^ACT
SexAI 2147 ACCAGGT A^CC[AT]GGT
SfuI 6407 TTCGAA TT^CGAA
SphI 6222 GCATGC GCATG^C
XcmI 5738 CCAAAACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 8 enzymes) #
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AclI 3299 AACGTT AA^CGTT
6532 AACGTT
Asp700I 407 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4649 GAAGCCATTC
HindIII 1306 AAGCTT A^AGCTT
3433 AAGCTT
Psp1406I 3299 AACGTT AA^CGTT
6532 AACGTT
PstI 383 CTGCAG CTGCA^G
6185 CTGCAG
SmlI 634 CTCAAG C^T[CT][GA]AG
4822 CTCAAG
XbaI 1659 TCTAGA T^CTAGA
4395 TCTAGA
XmnI 407 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4649 GAAGCCATTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII Hin1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII