Restriction map for
pF1KA0054
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
ApaLI AscI Asp718I AviII BbeI
BsaHI BsiEI BsiWI BsmBI BsrFI
BssHII BstEII BstPI Cfr10I CpoI
DraIII DrdI EagI EclXI Eco52I
EcoNI EcoO65I FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
Sse8387I StuI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 5625 CGCG CG^CG
AflII 49 CTTAAG C^TTAAG
AhdI 5752 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5518 CCTGAGG CC^T[ACGT]AGG
Aor51HI 5692 AGCGCT AGC^GCT
ApaI 5702 GGGCCC GGGCC^C
AseI 3871 ATTAAT AT^TAAT
AsnI 3871 ATTAAT AT^TAAT
AspEI 5752 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5255 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 460 CACGTG CAC^GTG
BfrI 49 CTTAAG C^TTAAG
BglII 1161 AGATCT A^GATCT
BlpI 5473 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5473 GCTCAGC GC^T[ACGT]AGC
BsaBI 901 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4708 GAGACC GGTCTC(1/5)
BspDI 1787 ATCGAT AT^CGAT
BspHI 3213 TCATGA T^CATGA
BspLU11I 243 ACATGT A^CATGT
BspMI 3585 ACCTGC ACCTGC(4/8)
BsrBI 2598 CCGCTC CCGCTC(-3/-3)
BssSI 2714 CACGAG CACGAG(-5/-1)
Bst1107I 4493 GTATAC GTA^TAC
BstUI 5625 CGCG CG^CG
BstZ17I 4493 GTATAC GTA^TAC
Bsu36I 5518 CCTGAGG CC^T[ACGT]AGG
CelII 5473 GCTCAGC GC^T[ACGT]AGC
ClaI 1787 ATCGAT AT^CGAT
DraI 5821 TTTAAA TTT^AAA
Eam1105I 5752 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5692 AGCGCT AGC^GCT
Eco81I 5518 CCTGAGG CC^T[ACGT]AGG
EcoRI 732 GAATTC G^AATTC
EcoRV 455 GATATC GAT^ATC
EcoT22I 1177 ATGCAT ATGCA^T
MamI 901 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1447 CAATTG C^AATTG
MunI 1447 CAATTG C^AATTG
MvnI 5625 CGCG CG^CG
NsiI 1177 ATGCAT ATGCA^T
PaeR7I 5545 CTCGAG C^TCGAG
PflFI 5255 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
PshAI 1584 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3871 ATTAAT AT^TAAT
RcaI 3213 TCATGA T^CATGA
SexAI 5256 ACCTGGT A^CC[AT]GGT
SmaI 3326 CCCGGG CCC^GGG
SnaBI 2987 TACGTA TAC^GTA
SpeI 436 ACTAGT A^CTAGT
SspI 1068 AATATT AAT^ATT
Tth111I 5255 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5545 CTCGAG C^TCGAG
XmaCI 3326 CCCGGG C^CCGGG
XmaI 3326 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 239 GTAGAC GT^[AC][GT]AC
4493 GTATAC
AccIII 3859 TCCGGA T^CCGGA
4347 TCCGGA
AclI 1211 AACGTT AA^CGTT
2022 AACGTT
Asp700I 2278 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4437 GAATAGCTTC
AvrII 514 CCTAGG C^CTAGG
5781 CCTAGG
BamHI 1914 GGATCC G^GATCC
5458 GGATCC
BcgI 4557 CGAGCATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
161 GCATCAAAATCG
BciVI 3643 GGATAC GTATCC(6/5)
331 GTATCC
BglI 280 GCCTTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5115 GCCTCACAGGC
BlnI 514 CCTAGG C^CTAGG
5781 CCTAGG
BpmI 4618 CTCCAG CTGGAG(16/14)
1487 CTGGAG
BsaWI 3859 TCCGGA [AT]^CCGG[AT]
4347 TCCGGA
BseAI 3859 TCCGGA T^CCGGA
4347 TCCGGA
BsgI 84 CTGCAC GTGCAG(16/14)
4301 GTGCAG
Bsp1407I 2727 TGTACA T^GTACA
5142 TGTACA
BspEI 3859 TCCGGA T^CCGGA
4347 TCCGGA
BsrGI 2727 TGTACA T^GTACA
5142 TGTACA
BstBI 2900 TTCGAA TT^CGAA
3881 TTCGAA
BstXI 2815 CCAGTAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3423 CCAGAATGATGG
MroI 3859 TCCGGA T^CCGGA
4347 TCCGGA
NspV 2900 TTCGAA TT^CGAA
3881 TTCGAA
PflMI 1874 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2391 CCAGGCCATGG
PpuMI 4275 GGGACCC [GA]G^G[AT]CC[CT]
5253 AGGACCT
Psp1406I 1211 AACGTT AA^CGTT
2022 AACGTT
PvuII 307 CAGCTG CAG^CTG
1978 CAGCTG
SacI 5655 GAGCTC GAGCT^C
5787 GAGCTC
SapI 11 GAAGAGC GCTCTTC(1/4)
1275 GAAGAGC
SfuI 2900 TTCGAA TT^CGAA
3881 TTCGAA
SphI 302 GCATGC GCATG^C
1179 GCATGC
SspBI 2727 TGTACA T^GTACA
5142 TGTACA
Van91I 1874 CCATACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2391 CCAGGCCATGG
XmnI 2278 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4437 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII