Restriction map for
pF1KA1763
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AvrII BbeI BbsI BcgI BglI
BlnI BpuAI BsaHI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoT22I
FseI HaeII Hin1I KasI KpnI
KspI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3844 GTAGAC GT^[AC][GT]AC
AccII 2442 CGCG CG^CG
AflII 1282 CTTAAG C^TTAAG
AflIII 3585 ACACGT A^C[GA][CT]GT
AocI 118 CCTCAGG CC^T[ACGT]AGG
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
AviII 3480 TGCGCA TGC^GCA
BamHI 1504 GGATCC G^GATCC
BbvCI 3853 GCTGAGG CCTCAGC(-5/-2)
BciVI 1253 GTATCC GTATCC(6/5)
BcnI 2355 CCGGG CC^[GC]GG
BfaI 799 CTAG C^TAG
BfrI 1282 CTTAAG C^TTAAG
BglII 1578 AGATCT A^GATCT
BsaBI 3750 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2225 ACCGGA [AT]^CCGG[AT]
BspHI 908 TCATGA T^CATGA
BstAPI 816 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1193 TTCGAA TT^CGAA
BstUI 2442 CGCG CG^CG
BstXI 3599 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 118 CCTCAGG CC^T[ACGT]AGG
DraI 3283 TTTAAA TTT^AAA
DsaI 2427 CCACGG C^C[GA][CT]GG
Eco81I 118 CCTCAGG CC^T[ACGT]AGG
EcoRV 1024 GATATC GAT^ATC
FspI 3480 TGCGCA TGC^GCA
HgaI 30 GACGC GACGC(5/10)
HpaI 3271 GTTAAC GTT^AAC
MaeI 799 CTAG C^TAG
MamI 3750 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2211 CAATTG C^AATTG
MunI 2211 CAATTG C^AATTG
MvnI 2442 CGCG CG^CG
NciI 2355 CCGGG CC^[GC]GG
NdeI 1955 CATATG CA^TATG
NspI 2718 GCATGT [GA]CATG^[CT]
NspV 1193 TTCGAA TT^CGAA
PpuMI 1600 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 733 ATTAAT AT^TAAT
RcaI 908 TCATGA T^CATGA
ScaI 3914 AGTACT AGT^ACT
SfuI 1193 TTCGAA TT^CGAA
StuI 2660 AGGCCT AGG^CCT
XcmI 761 CCACTTCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspI 3565 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3591 GACCGAGTC
BanI 1465 GGCACC G^G[CT][GA]CC
2627 GGCACC
BbrPI 3523 CACGTG CAC^GTG
3586 CACGTG
BlpI 3108 GCTGAGC GC^T[ACGT]AGC
3516 GCTCAGC
Bpu1102I 3108 GCTGAGC GC^T[ACGT]AGC
3516 GCTCAGC
BsaAI 3523 CACGTG [CT]AC^GT[GA]
3586 CACGTG
BsaI 209 GGTCTC GGTCTC(1/5)
2798 GGTCTC
BspMI 1330 GCAGGT ACCTGC(4/8)
3475 GCAGGT
BstEII 270 GGTCACC G^GT[ACGT]ACC
315 GGTGACC
BstPI 270 GGTCACC G^GT[ACGT]ACC
315 GGTGACC
CelII 3108 GCTGAGC GC^T[ACGT]AGC
3516 GCTCAGC
DraIII 1523 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2629 CACCATGTG
EcoO65I 270 GGTCACC G^GT[ACGT]ACC
315 GGTGACC
FauI 2701 CCCGC CCCGC(4/6)
2707 CCCGC
HapII 2226 CCGG C^CGG
2355 CCGG
HincII 850 GTCAAC GT[CT]^[GA]AC
3271 GTTAAC
HindII 850 GTCAAC GT[CT]^[GA]AC
3271 GTTAAC
HindIII 1497 AAGCTT A^AGCTT
3156 AAGCTT
HpaII 2226 CCGG C^CGG
2355 CCGG
MaeII 3524 ACGT A^CGT
3587 ACGT
MspI 2226 CCGG C^CGG
2355 CCGG
PflFI 3565 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3591 GACCGAGTC
PmaCI 3523 CACGTG CAC^GTG
3586 CACGTG
PmlI 3523 CACGTG CAC^GTG
3586 CACGTG
SapI 710 GAAGAGC GCTCTTC(1/4)
2745 GCTCTTC
Tth111I 3565 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3591 GACCGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbvI BclI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI