Restriction map for
pF1KA0627
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
BbeI BbrPI BcgI BfrI BglI
BlpI Bpu1102I BsaAI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
FspI HpaI KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 103 TCCGGA T^CCGGA
AcyI 1870 GACGCC G[GA]^CG[CT]C
Alw44I 3827 GTGCAC G^TGCAC
ApaLI 3827 GTGCAC G^TGCAC
AseI 3580 ATTAAT AT^TAAT
AsnI 3580 ATTAAT AT^TAAT
Asp700I 1853 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2022 CCTAGG C^CTAGG
BamHI 2421 GGATCC G^GATCC
BanII 1428 GAGCTC G[GA]GC[CT]^C
BciVI 1227 GGATAC GTATCC(6/5)
BglII 3481 AGATCT A^GATCT
BlnI 2022 CCTAGG C^CTAGG
BsaBI 1838 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1870 GACGCC G[GA]^CG[CT]C
BsaI 1385 GGTCTC GGTCTC(1/5)
BsaWI 103 TCCGGA [AT]^CCGG[AT]
BseAI 103 TCCGGA T^CCGGA
BsmBI 687 GAGACG CGTCTC(1/5)
BspEI 103 TCCGGA T^CCGGA
BsrFI 1376 GCCGGT [GA]^CCGG[CT]
BstBI 2636 TTCGAA TT^CGAA
Cfr10I 1376 GCCGGT [GA]^CCGG[CT]
DraI 3409 TTTAAA TTT^AAA
DraIII 1103 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 785 GAATTC G^AATTC
FauI 1175 GCGGG CCCGC(4/6)
Hin1I 1870 GACGCC G[GA]^CG[CT]C
MamI 1838 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 103 TCCGGA T^CCGGA
MspA1I 65 CAGCTG C[AC]G^C[GT]G
NcoI 2666 CCATGG C^CATGG
NdeI 1847 CATATG CA^TATG
NspV 2636 TTCGAA TT^CGAA
PaeR7I 3047 CTCGAG C^TCGAG
PpuMI 964 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3580 ATTAAT AT^TAAT
PvuII 65 CAGCTG CAG^CTG
SacI 1428 GAGCTC GAGCT^C
SalI 1739 GTCGAC G^TCGAC
SfuI 2636 TTCGAA TT^CGAA
StuI 2016 AGGCCT AGG^CCT
XbaI 1327 TCTAGA T^CTAGA
XhoI 3047 CTCGAG C^TCGAG
XmnI 1853 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 1017 GTCTAC GT^[AC][GT]AC
1739 GTCGAC
AvaI 2468 CTCGGG C^[CT]CG[GA]G
3047 CTCGAG
BalI 2408 TGGCCA TGG^CCA
3509 TGGCCA
BclI 513 TGATCA T^GATCA
2346 TGATCA
BcnI 1269 CCGGG CC^[GC]GG
1587 CCGGG
BsoBI 2468 CTCGGG C^[CT]CG[GA]G
3047 CTCGAG
BspHI 789 TCATGA T^CATGA
3434 TCATGA
BsrDI 3008 CATTGC GCAATG(2/0)
2648 GCAATG
CfoI 521 GCGC GCG^C
1179 GCGC
DraII 964 AGGTCCT [GA]G^G[ACGT]CC[CT]
1702 GGGGCCT
DsaI 1489 CCACGG C^C[GA][CT]GG
2666 CCATGG
EcoO109I 964 AGGTCCT [GA]G^G[ACGT]CC[CT]
1702 GGGGCCT
EcoT22I 1855 ATGCAT ATGCA^T
1883 ATGCAT
FbaI 513 TGATCA T^GATCA
2346 TGATCA
HaeII 520 GGCGCT [GA]GCGC^[CT]
1178 GGCGCT
HhaI 521 GCGC GCG^C
1179 GCGC
HinP1I 521 GCGC G^CGC
1179 GCGC
HincII 1739 GTCGAC GT[CT]^[GA]AC
3779 GTCAAC
HindII 1739 GTCGAC GT[CT]^[GA]AC
3779 GTCAAC
MaeII 690 ACGT A^CGT
3825 ACGT
MfeI 657 CAATTG C^AATTG
1995 CAATTG
MluNI 2408 TGGCCA TGG^CCA
3509 TGGCCA
MscI 2408 TGGCCA TGG^CCA
3509 TGGCCA
MunI 657 CAATTG C^AATTG
1995 CAATTG
NciI 1269 CCGGG CC^[GC]GG
1587 CCGGG
NsiI 1855 ATGCAT ATGCA^T
1883 ATGCAT
NspI 750 ACATGC [GA]CATG^[CT]
2138 GCATGT
PflMI 1294 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1714 CCAGGTTATGG
RcaI 789 TCATGA T^CATGA
3434 TCATGA
SspI 2323 AATATT AAT^ATT
3306 AATATT
Van91I 1294 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1714 CCAGGTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII EaeI EarI Eco81I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PshAI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII