Restriction map for
pF1KB9703
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI DsaI Eam1105I EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I KspI
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NcoI NdeI NdeII NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI Sau3AI
SbfI SexAI SfaNI SfiI SfuI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 484 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 237 AGCGCT AGC^GCT
BalI 620 TGGCCA TGG^CCA
BanII 649 GAGCTC G[GA]GC[CT]^C
BbsI 90 GTCTTC GAAGAC(2/6)
BbvCI 642 CCTCAGC CCTCAGC(-5/-2)
BpuAI 90 GTCTTC GAAGAC(2/6)
BsgI 655 GTGCAG GTGCAG(16/14)
BsiEI 224 CGGCCG CG[GA][CT]^CG
BsmBI 307 GAGACG CGTCTC(1/5)
BspMI 665 ACCTGC ACCTGC(4/8)
BsrFI 452 GCCGGC [GA]^CCGG[CT]
BsrI 87 CCAGT ACTGG(1/-1)
BstAPI 483 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 567 CATCC GGATG(2/0)
Cfr10I 452 GCCGGC [GA]^CCGG[CT]
EagI 224 CGGCCG C^GGCCG
EclXI 224 CGGCCG C^GGCCG
Eco47III 237 AGCGCT AGC^GCT
Eco52I 224 CGGCCG C^GGCCG
EcoNI 594 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 599 CCAAGG C^C[AT][AT]GG
FokI 567 CATCC GGATG(9/13)
MaeII 506 ACGT A^CGT
MluNI 620 TGGCCA TGG^CCA
MscI 620 TGGCCA TGG^CCA
MspA1I 487 CAGCTG C[AC]G^C[GT]G
NaeI 452 GCCGGC GCC^GGC
NgoMIV 452 GCCGGC G^CCGGC
NheI 234 GCTAGC G^CTAGC
NspI 524 GCATGC [GA]CATG^[CT]
PflMI 893 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 487 CAGCTG CAG^CTG
SacI 649 GAGCTC GAGCT^C
SgrAI 451 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 865 CTCAAG C^T[CT][GA]AG
SphI 524 GCATGC GCATG^C
SspI 877 AATATT AAT^ATT
StyI 599 CCAAGG C^C[AT][AT]GG
Van91I 893 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 19 GAGCAC G[AT]GC[AT]^C
649 GAGCTC
BglI 379 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
770 GCCATCGGGGC
BpmI 16 CTGGAG CTGGAG(16/14)
106 CTGGAG
BsiHKAI 19 GAGCAC G[AT]GC[AT]^C
649 GAGCTC
BsmAI 307 GAGAC GTCTC(1/5)
396 GTCTC
BsmFI 98 GGGAC GGGAC(10/14)
731 GGGAC
HphI 268 GGTGA GGTGA(8/7)
80 TCACC
NlaIII 525 CATG CATG^
845 CATG
PpuMI 98 GGGACCC [GA]G^G[AT]CC[CT]
911 AGGACCC
PstI 208 CTGCAG CTGCA^G
481 CTGCAG
ScaI 176 AGTACT AGT^ACT
390 AGTACT
SfcI 208 CTGCAG C^T[GA][CT]AG
481 CTGCAG
TaqI 62 TCGA T^CGA
548 TCGA
TthHB8I 62 TCGA T^CGA
548 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BbeI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BsiYI BslI BsoBI Bsp1286I BsrBI
BssKI BssSI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MaeI
MboII MnlI MspI MvaI MvnI
MwoI NarI NciI NlaIV RsaI
Sau96I ScrFI SfoI SmaI TseI
XmaCI XmaI