Restriction map for
pF1KB9738
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BalI BamHI BanI
BbeI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiWI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FbaI FokI FseI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 1246 GAATTC [GA]^AATT[CT]
Alw44I 1378 GTGCAC G^TGCAC
AlwI 610 GATCC GGATC(4/5)
ApaLI 1378 GTGCAC G^TGCAC
ApoI 1246 GAATTC [GA]^AATT[CT]
AspI 591 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1054 CACGTG CAC^GTG
BbvCI 1431 CCTCAGC CCTCAGC(-5/-2)
BglI 459 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 369 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 369 GCTCAGC GC^T[ACGT]AGC
BsaAI 1054 CACGTG [CT]AC^GT[GA]
BsiEI 344 CGGCCG CG[GA][CT]^CG
BsmBI 721 GAGACG CGTCTC(1/5)
BsmI 988 GCATTC GAATGC(1/-1)
Bsp1407I 881 TGTACA T^GTACA
BsrFI 265 GCCGGC [GA]^CCGG[CT]
BsrGI 881 TGTACA T^GTACA
BstAPI 68 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 609 AGATCC [GA]^GATC[CT]
CelII 369 GCTCAGC GC^T[ACGT]AGC
Cfr10I 265 GCCGGC [GA]^CCGG[CT]
EagI 344 CGGCCG C^GGCCG
EarI 1335 GAAGAG CTCTTC(1/4)
EclXI 344 CGGCCG C^GGCCG
Eco52I 344 CGGCCG C^GGCCG
EcoRI 1246 GAATTC G^AATTC
Ksp632I 1335 GAAGAG CTCTTC(1/4)
MflI 609 AGATCC [GA]^GATC[CT]
NaeI 265 GCCGGC GCC^GGC
NgoMIV 265 GCCGGC G^CCGGC
NspI 486 ACATGC [GA]CATG^[CT]
PflFI 591 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1054 CACGTG CAC^GTG
PmlI 1054 CACGTG CAC^GTG
SacI 1006 GAGCTC GAGCT^C
SapI 1335 GAAGAGC GCTCTTC(1/4)
SfiI 458 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 736 CCCGGG CCC^GGG
SspBI 881 TGTACA T^GTACA
StuI 182 AGGCCT AGG^CCT
Tsp509I 1247 AATT ^AATT
Tth111I 591 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 609 AGATCC [GA]^GATC[CT]
XmaCI 736 CCCGGG C^CCGGG
XmaI 736 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 30 ACGCGT A^C[GA][CT]GT
304 ACGCGT
Asp700I 132 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGGGCTTC
AspHI 1006 GAGCTC G[AT]GC[AT]^C
1378 GTGCAC
AviII 289 TGCGCA TGC^GCA
296 TGCGCA
BbsI 1225 GTCTTC GAAGAC(2/6)
1410 GTCTTC
BfaI 215 CTAG C^TAG
1384 CTAG
BpuAI 1225 GTCTTC GAAGAC(2/6)
1410 GTCTTC
BsgI 831 CTGCAC GTGCAG(16/14)
927 CTGCAC
BsiHKAI 1006 GAGCTC G[AT]GC[AT]^C
1378 GTGCAC
BsmAI 250 GAGAC GTCTC(1/5)
721 GAGAC
BspMI 203 GCAGGT ACCTGC(4/8)
981 GCAGGT
BstXI 1090 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1286 CCATCTACCTGG
DpnII 63 GATC ^GATC
610 GATC
DraIII 355 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
449 CACAGGGTG
DrdI 952 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1216 GACTTCAGAGTC
EaeI 208 TGGCCG [CT]^GGCC[GA]
344 CGGCCG
EcoNI 153 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
546 CCTGCTCCAGG
FspI 289 TGCGCA TGC^GCA
296 TGCGCA
MaeI 215 CTAG C^TAG
1384 CTAG
MboI 63 GATC ^GATC
610 GATC
MluI 30 ACGCGT A^CGCGT
304 ACGCGT
NdeII 63 GATC ^GATC
610 GATC
PflMI 448 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1233 CCAAGAGCTGG
PstI 88 CTGCAG CTGCA^G
901 CTGCAG
Sau3AI 63 GATC ^GATC
610 GATC
SfaNI 318 GATGC GCATC(5/9)
173 GCATC
SfcI 88 CTGCAG C^T[GA][CT]AG
901 CTGCAG
TaqI 1214 TCGA T^CGA
1475 TCGA
TthHB8I 1214 TCGA T^CGA
1475 TCGA
Van91I 448 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1233 CCAAGAGCTGG
XmnI 132 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KspI MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PpuMI PvuII RsaI SacII Sau96I
ScrFI StyI TseI Tsp45I TspRI