Restriction map for
pF1KB5696
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BclI
BcnI BfaI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfaNI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TspRI Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 398 CCGC CCGC(-3/-1)
AfaI 289 GTAC GT^AC
AlwI 315 GATCC GGATC(4/5)
AspI 500 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 236 CTCGAG C^[CT]CG[GA]G
BanII 158 GAGCCC G[GA]GC[CT]^C
BbsI 346 GTCTTC GAAGAC(2/6)
BbvCI 388 GCTGAGG CCTCAGC(-5/-2)
BbvI 67 GCAGC GCAGC(8/12)
BcgI 228 GCAGATGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 690 GGATAC GTATCC(6/5)
BlpI 46 GCTGAGC GC^T[ACGT]AGC
BmyI 158 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
BpuAI 346 GTCTTC GAAGAC(2/6)
BsaJI 696 CCAAGG C^C[ACGT][ACGT]GG
BseRI 115 GAGGAG GAGGAG(10/8)
BsiYI 378 CCGTTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 378 CCGTTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 236 CTCGAG C^[CT]CG[GA]G
Bsp1286I 158 GAGCCC G[AGT]GC[ACT]^C
BstF5I 490 CATCC GGATG(2/0)
CelII 46 GCTGAGC GC^T[ACGT]AGC
Cfr13I 510 GGCCC G^G[ACGT]CC
EaeI 35 TGGCCG [CT]^GGCC[GA]
EarI 622 GAAGAG CTCTTC(1/4)
EcoRV 274 GATATC GAT^ATC
EcoT14I 696 CCAAGG C^C[AT][AT]GG
FokI 490 CATCC GGATG(9/13)
HindIII 452 AAGCTT A^AGCTT
HphI 403 GGTGA GGTGA(8/7)
Ksp632I 622 GAAGAG CTCTTC(1/4)
MspA1I 398 CCGCTG C[AC]G^C[GT]G
NlaIV 157 GGAGCC GG[ACGT]^[ACGT]CC
PaeR7I 236 CTCGAG C^TCGAG
PflFI 500 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 625 GAGTC GAGTC(4/5)
RsaI 289 GTAC GT^AC
Sau96I 510 GGCCC G^G[ACGT]CC
SfiI 27 GGCCAAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 696 CCAAGG C^C[AT][AT]GG
TseI 67 GCAGC G^C[AT]GC
Tsp45I 432 GTCAC ^GT[GC]AC
Tth111I 500 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 236 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BglI 28 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
37 GCCGAGCAGGC
BsrDI 597 CATTGC GCAATG(2/0)
281 GCAATG
BsrI 33 ACTGG ACTGG(1/-1)
501 ACTGG
DpnII 315 GATC ^GATC
427 GATC
Fnu4HI 67 GCAGC GC^[ACGT]GC
397 GCCGC
ItaI 67 GCAGC GC^[ACGT]GC
397 GCCGC
MboI 315 GATC ^GATC
427 GATC
MseI 515 TTAA T^TAA
616 TTAA
NdeII 315 GATC ^GATC
427 GATC
NlaIII 63 CATG CATG^
75 CATG
Sau3AI 315 GATC ^GATC
427 GATC
SmlI 186 CTCAAG C^T[CT][GA]AG
236 CTCGAG
TaqI 237 TCGA T^CGA
425 TCGA
TfiI 123 GAATC G^A[AT]TC
540 GATTC
Tru9I 515 TTAA T^TAA
616 TTAA
TthHB8I 237 TCGA T^CGA
425 TCGA
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AluI BsmAI BsmBI Cac8I DdeI
HaeIII HinfI MaeIII MboII MnlI
MwoI Tsp509I