Restriction map for
pF1KB7109
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 493 AAATTC [GA]^AATT[CT]
AflII 285 CTTAAG C^TTAAG
AluI 72 AGCT AG^CT
AlwI 6 GGATC GGATC(4/5)
ApaI 550 GGGCCC GGGCC^C
ApoI 493 AAATTC [GA]^AATT[CT]
BalI 647 TGGCCA TGG^CCA
BanII 550 GGGCCC G[GA]GC[CT]^C
BbsI 414 GAAGAC GAAGAC(2/6)
BfrI 285 CTTAAG C^TTAAG
BmyI 550 GGGCCC G[AGT]GC[ACT]^C
BpuAI 414 GAAGAC GAAGAC(2/6)
BsaJI 242 CCCTGG C^C[ACGT][ACGT]GG
BsgI 135 GTGCAG GTGCAG(16/14)
BsmFI 641 GGGAC GGGAC(10/14)
Bsp1286I 550 GGGCCC G[AGT]GC[ACT]^C
BspMI 626 ACCTGC ACCTGC(4/8)
BsrDI 520 CATTGC GCAATG(2/0)
BstAPI 328 GCAGTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 702 TTTAAA TTT^AAA
DraII 550 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 647 TGGCCA [CT]^GGCC[GA]
EcoO109I 550 GGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 77 GTCAAC GT[CT]^[GA]AC
HindII 77 GTCAAC GT[CT]^[GA]AC
MaeII 39 ACGT A^CGT
MluNI 647 TGGCCA TGG^CCA
MscI 647 TGGCCA TGG^CCA
MspA1I 71 CAGCTG C[AC]G^C[GT]G
PvuII 71 CAGCTG CAG^CTG
SmlI 285 CTTAAG C^T[CT][GA]AG
SspI 196 AATATT AAT^ATT
Tsp509I 494 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 525 CCGC CCGC(-3/-1)
603 GCGG
BfaI 35 CTAG C^TAG
665 CTAG
BsiYI 149 CCAAAGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
404 CCAAAAGTGGG
BslI 149 CCAAAGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
404 CCAAAAGTGGG
BsmAI 281 GAGAC GTCTC(1/5)
696 GTCTC
BssKI 94 CCTGG ^CC[ACGT]GG
243 CCTGG
BstNI 94 CCTGG CC^[AT]GG
243 CCTGG
Cac8I 656 GCCAGC GC[ACGT]^[ACGT]GC
660 GCCTGC
Cfr13I 550 GGGCC G^G[ACGT]CC
551 GGCCC
DpnII 7 GATC ^GATC
668 GATC
EcoRII 94 CCTGG ^CC[AT]GG
243 CCTGG
HaeIII 551 GGCC GG^CC
648 GGCC
MaeI 35 CTAG C^TAG
665 CTAG
MboI 7 GATC ^GATC
668 GATC
MvaI 94 CCTGG CC^[AT]GG
243 CCTGG
NdeII 7 GATC ^GATC
668 GATC
NlaIV 447 GGTTCC GG[ACGT]^[ACGT]CC
550 GGGCCC
NspI 432 ACATGC [GA]CATG^[CT]
652 ACATGC
PleI 454 GACTC GAGTC(4/5)
673 GACTC
Sau3AI 7 GATC ^GATC
668 GATC
Sau96I 550 GGGCC G^G[ACGT]CC
551 GGCCC
ScrFI 94 CCTGG CC^[ACGT]GG
243 CCTGG
TfiI 262 GATTC G^A[AT]TC
369 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BbvI BsrI BstF5I DdeI Fnu4HI
FokI HinfI ItaI MaeIII MboII
MnlI MseI MwoI NlaIII Tru9I
TseI Tsp45I TspRI