Restriction map for
pF1KB8692
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BalI BbrPI BcgI BciVI BglI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HpaI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PstI PvuI RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 72 enzymes) #
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AccII 22 CGCG CG^CG
AclI 1376 AACGTT AA^CGTT
AcsI 915 AAATTT [GA]^AATT[CT]
AcyI 509 GGCGCC G[GA]^CG[CT]C
AflII 982 CTTAAG C^TTAAG
AhdI 881 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 946 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 580 CCTCAGG CC^T[ACGT]AGG
Aor51HI 522 AGCGCT AGC^GCT
ApoI 915 AAATTT [GA]^AATT[CT]
Asp700I 1006 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 881 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 336 GAGCTC G[AT]GC[AT]^C
AvrII 1267 CCTAGG C^CTAGG
BamHI 369 GGATCC G^GATCC
BanI 509 GGCGCC G^G[CT][GA]CC
BanII 336 GAGCTC G[GA]GC[CT]^C
BbeI 509 GGCGCC GGCGC^C
BbvCI 355 GCTGAGG CCTCAGC(-5/-2)
BclI 920 TGATCA T^GATCA
BfrI 982 CTTAAG C^TTAAG
BglII 1086 AGATCT A^GATCT
BlnI 1267 CCTAGG C^CTAGG
BmyI 336 GAGCTC G[AGT]GC[ACT]^C
BpmI 730 CTGGAG CTGGAG(16/14)
BsaBI 1285 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 509 GGCGCC G[GA]^CG[CT]C
BsgI 1406 GTGCAG GTGCAG(16/14)
BsiHKAI 336 GAGCTC G[AT]GC[AT]^C
BsmBI 209 GAGACG CGTCTC(1/5)
Bsp1286I 336 GAGCTC G[AGT]GC[ACT]^C
BspHI 153 TCATGA T^CATGA
BspMI 58 GCAGGT ACCTGC(4/8)
BsrFI 440 GCCGGC [GA]^CCGG[CT]
BstUI 22 CGCG CG^CG
Bsu36I 580 CCTCAGG CC^T[ACGT]AGG
Cfr10I 440 GCCGGC [GA]^CCGG[CT]
DraII 60 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 694 GACCTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 881 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 522 AGCGCT AGC^GCT
Eco81I 580 CCTCAGG CC^T[ACGT]AGG
EcoO109I 60 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 292 GCGGG CCCGC(4/6)
FbaI 920 TGATCA T^GATCA
Hin1I 509 GGCGCC G[GA]^CG[CT]C
HindIII 1429 AAGCTT A^AGCTT
KasI 509 GGCGCC G^GCGCC
MaeII 1377 ACGT A^CGT
MamI 1285 GATCGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 22 CGCG CG^CG
NaeI 440 GCCGGC GCC^GGC
NarI 509 GGCGCC GG^CGCC
NgoMIV 440 GCCGGC G^CCGGC
NheI 246 GCTAGC G^CTAGC
PflMI 871 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 60 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 600 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1376 AACGTT AA^CGTT
PvuII 41 CAGCTG CAG^CTG
RcaI 153 TCATGA T^CATGA
SacI 336 GAGCTC GAGCT^C
ScaI 1130 AGTACT AGT^ACT
SfaNI 991 GATGC GCATC(5/9)
SfoI 509 GGCGCC GGC^GCC
SmaI 167 CCCGGG CCC^GGG
TaqI 829 TCGA T^CGA
TthHB8I 829 TCGA T^CGA
Van91I 871 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 167 CCCGGG C^CCGGG
XmaI 167 CCCGGG C^CCGGG
XmnI 1006 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 167 CCCGGG C^[CT]CG[GA]G
434 CCCGAG
AvaII 61 GGTCC G^G[AT]CC
797 GGACC
BbsI 691 GAAGAC GAAGAC(2/6)
1147 GAAGAC
BpuAI 691 GAAGAC GAAGAC(2/6)
1147 GAAGAC
BsmAI 209 GAGAC GTCTC(1/5)
598 GAGAC
BsoBI 167 CCCGGG C^[CT]CG[GA]G
434 CCCGAG
BsrI 374 CCAGT ACTGG(1/-1)
574 CCAGT
EcoT14I 53 CCAAGG C^C[AT][AT]GG
1267 CCTAGG
HaeII 509 GGCGCC [GA]GCGC^[CT]
522 AGCGCT
MaeIII 904 GTTAC ^GT[ACGT]AC
1081 GTTAC
MspA1I 41 CAGCTG C[AC]G^C[GT]G
505 CAGCGG
NlaIII 154 CATG CATG^
877 CATG
PleI 447 GAGTC GAGTC(4/5)
866 GAGTC
SapI 1322 GAAGAGC GCTCTTC(1/4)
1369 GAAGAGC
SmlI 792 CTTGAG C^T[CT][GA]AG
982 CTTAAG
StuI 359 AGGCCT AGG^CCT
560 AGGCCT
StyI 53 CCAAGG C^C[AT][AT]GG
1267 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BfaI BsaJI BseRI BsiYI
BslI BsmFI BsmI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI XhoII