Restriction map for
pF1KA0133
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# NO CUT ENZYMES ( 67 enzymes) #
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Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I AspI AviII AvrII
BcgI BlnI BsaI BseAI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BssHII Bst1107I BstZ17I CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII ScaI SfiI
SgrAI SnaBI SspI StuI SwaI
Tth111I XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 4453 GACGTC GACGT^C
AccI 390 GTCGAC GT^[AC][GT]AC
AccII 3504 CGCG CG^CG
AflII 28 CTTAAG C^TTAAG
Alw44I 4271 GTGCAC G^TGCAC
ApaLI 4271 GTGCAC G^TGCAC
AseI 277 ATTAAT AT^TAAT
AsnI 277 ATTAAT AT^TAAT
Asp700I 4179 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 340 GGATCC G^GATCC
BbeI 3329 GGCGCC GGCGC^C
BciVI 2278 GGATAC GTATCC(6/5)
BclI 3513 TGATCA T^GATCA
BfrI 28 CTTAAG C^TTAAG
BglII 1919 AGATCT A^GATCT
BsaBI 3030 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2072 TCCGGT [AT]^CCGG[AT]
Bsp1407I 4292 TGTACA T^GTACA
BspDI 1306 ATCGAT AT^CGAT
BspLU11I 3773 ACATGT A^CATGT
BsrFI 1215 ACCGGC [GA]^CCGG[CT]
BsrGI 4292 TGTACA T^GTACA
BstBI 1328 TTCGAA TT^CGAA
BstEII 4377 GGTGACC G^GT[ACGT]ACC
BstPI 4377 GGTGACC G^GT[ACGT]ACC
BstUI 3504 CGCG CG^CG
Cfr10I 1215 ACCGGC [GA]^CCGG[CT]
ClaI 1306 ATCGAT AT^CGAT
EcoO65I 4377 GGTGACC G^GT[ACGT]ACC
EcoRV 1245 GATATC GAT^ATC
FbaI 3513 TGATCA T^GATCA
KasI 3329 GGCGCC G^GCGCC
MamI 3030 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3504 CGCG CG^CG
NarI 3329 GGCGCC GG^CGCC
NspV 1328 TTCGAA TT^CGAA
PpuMI 3201 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 277 ATTAAT AT^TAAT
SalI 390 GTCGAC G^TCGAC
SapI 4119 GCTCTTC GCTCTTC(1/4)
SexAI 3181 ACCAGGT A^CC[AT]GGT
SfoI 3329 GGCGCC GGC^GCC
SfuI 1328 TTCGAA TT^CGAA
SmaI 3320 CCCGGG CCC^GGG
SpeI 2065 ACTAGT A^CTAGT
SphI 1498 GCATGC GCATG^C
SspBI 4292 TGTACA T^GTACA
XbaI 933 TCTAGA T^CTAGA
XcmI 1061 CCACAGAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3320 CCCGGG C^CCGGG
XmaI 3320 CCCGGG C^CCGGG
XmnI 4179 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 3329 GGCGCC G[GA]^CG[CT]C
4453 GACGTC
AhdI 1534 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3758 GACTGGATGTC
AocI 621 CCTGAGG CC^T[ACGT]AGG
4144 CCTAAGG
AspEI 1534 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3758 GACTGGATGTC
BanI 2476 GGTGCC G^G[CT][GA]CC
3329 GGCGCC
BbrPI 1174 CACGTG CAC^GTG
1536 CACGTG
BglI 1342 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1597 GCCGACATGGC
BsaHI 3329 GGCGCC G[GA]^CG[CT]C
4453 GACGTC
BsmAI 4493 GAGAC GTCTC(1/5)
2332 GTCTC
BsmI 2462 GCATTC GAATGC(1/-1)
3082 GCATTC
BssSI 4310 CACGAG CACGAG(-5/-1)
1735 CTCGTG
Bsu36I 621 CCTGAGG CC^T[ACGT]AGG
4144 CCTAAGG
CfoI 1351 GCGC GCG^C
3330 GCGC
DraIII 3842 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4111 CACAACGTG
Eam1105I 1534 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3758 GACTGGATGTC
Eco81I 621 CCTGAGG CC^T[ACGT]AGG
4144 CCTAAGG
EcoNI 1017 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3456 CCTCTACCAGG
EcoRI 1136 GAATTC G^AATTC
2986 GAATTC
HaeII 1350 GGCGCT [GA]GCGC^[CT]
3329 GGCGCC
HhaI 1351 GCGC GCG^C
3330 GCGC
Hin1I 3329 GGCGCC G[GA]^CG[CT]C
4453 GACGTC
HinP1I 1351 GCGC G^CGC
3330 GCGC
HincII 390 GTCGAC GT[CT]^[GA]AC
562 GTCAAC
HindII 390 GTCGAC GT[CT]^[GA]AC
562 GTCAAC
NspI 1498 GCATGC [GA]CATG^[CT]
3773 ACATGT
PmaCI 1174 CACGTG CAC^GTG
1536 CACGTG
PmlI 1174 CACGTG CAC^GTG
1536 CACGTG
PshAI 2488 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3379 GACCTGGGTC
SacI 1732 GAGCTC GAGCT^C
4507 GAGCTC
SbfI 470 CCTGCAGG CCTGCA^GG
1017 CCTGCAGG
Sse8387I 470 CCTGCAGG CCTGCA^GG
1017 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII