Restriction map for
pF1KA0913
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AgeI ApoI AscI AseI AsnI
Asp700I AspI BamHI BbrPI BsaBI
BsiEI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I ClaI DraI EagI EclXI
Eco52I EcoRI EcoRV FseI HpaI
KspI MamI MfeI MluI MunI
MvnI NaeI NdeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacII SalI
SbfI SexAI SfuI SgrAI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 193 GGTACC G^GTACC
AflIII 2845 ACACGT A^C[GA][CT]GT
AhdI 4827 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5020 GTGCAC G^TGCAC
ApaLI 5020 GTGCAC G^TGCAC
Asp718I 193 GGTACC G^GTACC
AspEI 4827 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2244 TGCGCA TGC^GCA
AvrII 4569 CCTAGG C^CTAGG
BbeI 1319 GGCGCC GGCGC^C
BciVI 2362 GTATCC GTATCC(6/5)
BglII 2918 AGATCT A^GATCT
BlnI 4569 CCTAGG C^CTAGG
BsiWI 1164 CGTACG C^GTACG
BssSI 2367 CACGAG CACGAG(-5/-1)
BstEII 2860 GGTGACC G^GT[ACGT]ACC
BstPI 2860 GGTGACC G^GT[ACGT]ACC
CpoI 56 CGGACCG CG^G[AT]CCG
DrdI 1261 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4827 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2860 GGTGACC G^GT[ACGT]ACC
EcoT22I 4010 ATGCAT ATGCA^T
FspI 2244 TGCGCA TGC^GCA
HindIII 2908 AAGCTT A^AGCTT
KasI 1319 GGCGCC G^GCGCC
KpnI 193 GGTACC GGTAC^C
NarI 1319 GGCGCC GG^CGCC
NheI 2301 GCTAGC G^CTAGC
NsiI 4010 ATGCAT ATGCA^T
PaeR7I 5365 CTCGAG C^TCGAG
RsrII 56 CGGACCG CG^G[AT]CCG
ScaI 853 AGTACT AGT^ACT
SfiI 1960 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1319 GGCGCC GGC^GCC
SnaBI 1166 TACGTA TAC^GTA
XhoI 5365 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 816 TCCGGA T^CCGGA
5249 TCCGGA
AflII 2911 CTTAAG C^TTAAG
3375 CTTAAG
Aor51HI 35 AGCGCT AGC^GCT
5162 AGCGCT
BclI 2882 TGATCA T^GATCA
3647 TGATCA
BfrI 2911 CTTAAG C^TTAAG
3375 CTTAAG
BsaWI 816 TCCGGA [AT]^CCGG[AT]
5249 TCCGGA
BseAI 816 TCCGGA T^CCGGA
5249 TCCGGA
BspEI 816 TCCGGA T^CCGGA
5249 TCCGGA
BsrFI 1586 ACCGGC [GA]^CCGG[CT]
4299 GCCGGT
Cfr10I 1586 ACCGGC [GA]^CCGG[CT]
4299 GCCGGT
Eco47III 35 AGCGCT AGC^GCT
5162 AGCGCT
EcoNI 499 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2400 CCTGAGCAAGG
FbaI 2882 TGATCA T^GATCA
3647 TGATCA
HincII 786 GTCAAC GT[CT]^[GA]AC
2421 GTTGAC
HindII 786 GTCAAC GT[CT]^[GA]AC
2421 GTTGAC
MroI 816 TCCGGA T^CCGGA
5249 TCCGGA
MseI 2912 TTAA T^TAA
3376 TTAA
SacI 724 GAGCTC GAGCT^C
812 GAGCTC
Tru9I 2912 TTAA T^TAA
3376 TTAA
Tsp509I 154 AATT ^AATT
321 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI