Restriction map for
pF1KE0199
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI AspI AvrII
BalI BbeI BbrPI BfrI BglII
BlnI BsaAI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HaeII HindIII HpaI
KasI KspI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NgoMIV NheI NotI NsiI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1576 GGTACC G^GTACC
AccI 2050 GTCTAC GT^[AC][GT]AC
AcyI 1168 GACGCC G[GA]^CG[CT]C
AgeI 1573 ACCGGT A^CCGGT
AhdI 1539 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 795 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1576 GGTACC G^GTACC
AspEI 1539 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 875 TGCGCA TGC^GCA
BamHI 624 GGATCC G^GATCC
BbvCI 834 GCTGAGG CCTCAGC(-5/-2)
BcgI 1050 GCACCAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3195 TGATCA T^GATCA
BsaBI 338 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1168 GACGCC G[GA]^CG[CT]C
BsaWI 1573 ACCGGT [AT]^CCGG[AT]
BsmI 2082 GAATGC GAATGC(1/-1)
BsrFI 1573 ACCGGT [GA]^CCGG[CT]
BssSI 1003 CACGAG CACGAG(-5/-1)
BstEII 1902 GGTCACC G^GT[ACGT]ACC
BstPI 1902 GGTCACC G^GT[ACGT]ACC
CfoI 876 GCGC GCG^C
Cfr10I 1573 ACCGGT [GA]^CCGG[CT]
EaeI 1528 CGGCCA [CT]^GGCC[GA]
Eam1105I 1539 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3082 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1902 GGTCACC G^GT[ACGT]ACC
EcoRI 3298 GAATTC G^AATTC
FbaI 3195 TGATCA T^GATCA
FspI 875 TGCGCA TGC^GCA
HhaI 876 GCGC GCG^C
Hin1I 1168 GACGCC G[GA]^CG[CT]C
HinP1I 876 GCGC G^CGC
HincII 228 GTTGAC GT[CT]^[GA]AC
HindII 228 GTTGAC GT[CT]^[GA]AC
KpnI 1576 GGTACC GGTAC^C
MaeII 910 ACGT A^CGT
MamI 338 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2840 CATATG CA^TATG
NruI 334 TCGCGA TCG^CGA
PaeR7I 661 CTCGAG C^TCGAG
PinAI 1573 ACCGGT A^CCGGT
SapI 2113 GAAGAGC GCTCTTC(1/4)
XhoI 661 CTCGAG C^TCGAG
XmnI 795 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 335 CGCG CG^CG
1594 CGCG
AflIII 992 ACATGT A^C[GA][CT]GT
1143 ACATGT
Alw44I 757 GTGCAC G^TGCAC
906 GTGCAC
AlwNI 2213 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3169 CAGGGGCTG
AocI 1500 CCTCAGG CC^T[ACGT]AGG
3040 CCTGAGG
ApaLI 757 GTGCAC G^TGCAC
906 GTGCAC
BciVI 1882 GGATAC GTATCC(6/5)
1875 GTATCC
BlpI 2218 GCTGAGC GC^T[ACGT]AGC
2683 GCTCAGC
Bpu1102I 2218 GCTGAGC GC^T[ACGT]AGC
2683 GCTCAGC
BsaI 172 GAGACC GGTCTC(1/5)
1546 GGTCTC
BspLU11I 992 ACATGT A^CATGT
1143 ACATGT
BstUI 335 CGCG CG^CG
1594 CGCG
Bsu36I 1500 CCTCAGG CC^T[ACGT]AGG
3040 CCTGAGG
CelII 2218 GCTGAGC GC^T[ACGT]AGC
2683 GCTCAGC
EarI 1481 CTCTTC CTCTTC(1/4)
2113 GAAGAG
Eco81I 1500 CCTCAGG CC^T[ACGT]AGG
3040 CCTGAGG
HgaI 957 GACGC GACGC(5/10)
1168 GACGC
Ksp632I 1481 CTCTTC CTCTTC(1/4)
2113 GAAGAG
MvnI 335 CGCG CG^CG
1594 CGCG
NcoI 2090 CCATGG C^CATGG
2135 CCATGG
StuI 2306 AGGCCT AGG^CCT
2632 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI