Restriction map for
pF1KB7614
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI HincII
HindII HpaI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 961 GGTACC G^GTACC
AcsI 738 GAATTT [GA]^AATT[CT]
AcyI 842 GGCGCC G[GA]^CG[CT]C
AflIII 587 ACATGT A^C[GA][CT]GT
AlwNI 158 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 738 GAATTT [GA]^AATT[CT]
Asp718I 961 GGTACC G^GTACC
BbeI 842 GGCGCC GGCGC^C
BcgI 438 CGAGCAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 250 CTAG C^TAG
BsaHI 842 GGCGCC G[GA]^CG[CT]C
BsmI 1071 GCATTC GAATGC(1/-1)
BspLU11I 587 ACATGT A^CATGT
BsrFI 839 ACCGGC [GA]^CCGG[CT]
BssSI 676 CTCGTG CACGAG(-5/-1)
BstXI 792 CCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 843 GCGC GCG^C
Cfr10I 839 ACCGGC [GA]^CCGG[CT]
EarI 465 GAAGAG CTCTTC(1/4)
EcoT22I 1069 ATGCAT ATGCA^T
HaeII 842 GGCGCC [GA]GCGC^[CT]
HhaI 843 GCGC GCG^C
Hin1I 842 GGCGCC G[GA]^CG[CT]C
HinP1I 843 GCGC G^CGC
HindIII 108 AAGCTT A^AGCTT
KasI 842 GGCGCC G^GCGCC
KpnI 961 GGTACC GGTAC^C
Ksp632I 465 GAAGAG CTCTTC(1/4)
MaeI 250 CTAG C^TAG
NarI 842 GGCGCC GG^CGCC
NsiI 1069 ATGCAT ATGCA^T
NspI 587 ACATGT [GA]CATG^[CT]
PshAI 923 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 62 GGCCAGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 842 GGCGCC GGC^GCC
XcmI 114 CCAAGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 91 GATCC GGATC(4/5)
470 GGATC
AocI 283 CCTGAGG CC^T[ACGT]AGG
1018 CCTGAGG
ApaI 229 GGGCCC GGGCC^C
949 GGGCCC
AvaI 436 CTCGAG C^[CT]CG[GA]G
805 CTCGAG
BglI 63 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
845 GCCAGAAGGGC
BlpI 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
Bpu1102I 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
BsaI 271 GAGACC GGTCTC(1/5)
905 GGTCTC
BseRI 1002 CTCCTC GAGGAG(10/8)
400 GAGGAG
BsoBI 436 CTCGAG C^[CT]CG[GA]G
805 CTCGAG
BsrI 704 CCAGT ACTGG(1/-1)
925 CCAGT
BstF5I 215 GGATG GGATG(2/0)
813 GGATG
Bsu36I 283 CCTGAGG CC^T[ACGT]AGG
1018 CCTGAGG
Cac8I 681 GCAGGC GC[ACGT]^[ACGT]GC
854 GCAAGC
CelII 105 GCTAAGC GC^T[ACGT]AGC
573 GCTTAGC
Eco81I 283 CCTGAGG CC^T[ACGT]AGG
1018 CCTGAGG
EcoT14I 114 CCAAGG C^C[AT][AT]GG
258 CCAAGG
FauI 192 CCCGC CCCGC(4/6)
4 GCGGG
FokI 215 GGATG GGATG(9/13)
813 GGATG
MboII 465 GAAGA GAAGA(8/7)
648 TCTTC
PaeR7I 436 CTCGAG C^TCGAG
805 CTCGAG
PflMI 43 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
967 CCAGGCTATGG
PleI 299 GAGTC GAGTC(4/5)
403 GAGTC
SmlI 436 CTCGAG C^T[CT][GA]AG
805 CTCGAG
StyI 114 CCAAGG C^C[AT][AT]GG
258 CCAAGG
TfiI 137 GAATC G^A[AT]TC
177 GATTC
Tsp45I 349 GTCAC ^GT[GC]AC
1048 GTCAC
Tsp509I 443 AATT ^AATT
739 AATT
Van91I 43 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
967 CCAGGCTATGG
XhoI 436 CTCGAG C^TCGAG
805 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI Asp700I AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstNI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TseI TspRI TthHB8I XmnI