Restriction map for
pF1KB9415
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BclI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspMI BsrDI
BssHII BstBI BstEII BstPI BstUI
Bsu36I CfoI ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KspI MamI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StyI SwaI Tsp45I Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 1967 GGTACC G^GTACC
AccI 317 GTATAC GT^[AC][GT]AC
AflII 1933 CTTAAG C^TTAAG
Alw44I 1356 GTGCAC G^TGCAC
AlwNI 1669 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1356 GTGCAC G^TGCAC
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
Asp700I 4 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1967 GGTACC G^GTACC
AspHI 1356 GTGCAC G[AT]GC[AT]^C
AvaI 487 CCCGAG C^[CT]CG[GA]G
BanI 1967 GGTACC G^G[CT][GA]CC
BbsI 1999 GAAGAC GAAGAC(2/6)
BciVI 1052 GTATCC GTATCC(6/5)
BcnI 974 CCGGG CC^[GC]GG
BfrI 1933 CTTAAG C^TTAAG
BglII 945 AGATCT A^GATCT
BmyI 1356 GTGCAC G[AGT]GC[ACT]^C
BpmI 923 CTCCAG CTGGAG(16/14)
BpuAI 1999 GAAGAC GAAGAC(2/6)
BsaI 159 GAGACC GGTCTC(1/5)
BsaJI 785 CCAGGG C^C[ACGT][ACGT]GG
BseRI 1624 GAGGAG GAGGAG(10/8)
BsiHKAI 1356 GTGCAC G[AT]GC[AT]^C
BsoBI 487 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1356 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 177 TGTACA T^GTACA
BsrFI 139 GCCGGT [GA]^CCGG[CT]
BsrGI 177 TGTACA T^GTACA
BssSI 883 CTCGTG CACGAG(-5/-1)
Bst1107I 317 GTATAC GTA^TAC
BstAPI 1036 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 785 CCAGG CC^[AT]GG
BstXI 1920 CCAGAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 317 GTATAC GTA^TAC
Cfr10I 139 GCCGGT [GA]^CCGG[CT]
DraI 660 TTTAAA TTT^AAA
DraII 878 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1281 CTCTTC CTCTTC(1/4)
EcoNI 76 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 878 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 785 CCAGG ^CC[AT]GG
HpaI 606 GTTAAC GTT^AAC
KpnI 1967 GGTACC GGTAC^C
Ksp632I 1281 CTCTTC CTCTTC(1/4)
MaeII 828 ACGT A^CGT
MaeIII 382 GTAAC ^GT[ACGT]AC
MvaI 785 CCAGG CC^[AT]GG
NciI 974 CCGGG CC^[GC]GG
NdeI 53 CATATG CA^TATG
NheI 804 GCTAGC G^CTAGC
PflMI 1001 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 878 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 589 ATTAAT AT^TAAT
SapI 1280 GCTCTTC GCTCTTC(1/4)
SfiI 2029 GGCCAAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 272 GCATGC GCATG^C
SspBI 177 TGTACA T^GTACA
SspI 1657 AATATT AAT^ATT
StuI 1422 AGGCCT AGG^CCT
Van91I 1001 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1114 CCAAATCGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 178 GTAC GT^AC
1968 GTAC
AflIII 106 ACATGT A^C[GA][CT]GT
392 ACATGT
BlpI 1157 GCTTAGC GC^T[ACGT]AGC
1616 GCTCAGC
Bpu1102I 1157 GCTTAGC GC^T[ACGT]AGC
1616 GCTCAGC
BsiYI 76 CCTTCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1001 CCAAACACTGG
BslI 76 CCTTCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1001 CCAAACACTGG
BspLU11I 106 ACATGT A^CATGT
392 ACATGT
BsrBI 1614 CCGCTC CCGCTC(-3/-3)
1842 CCGCTC
BsrI 1007 ACTGG ACTGG(1/-1)
1693 ACTGG
BssKI 785 CCAGG ^CC[ACGT]GG
974 CCGGG
CelII 1157 GCTTAGC GC^T[ACGT]AGC
1616 GCTCAGC
EcoRI 330 GAATTC G^AATTC
1338 GAATTC
FauI 1841 CCCGC CCCGC(4/6)
1761 GCGGG
HincII 453 GTCAAC GT[CT]^[GA]AC
606 GTTAAC
HindII 453 GTCAAC GT[CT]^[GA]AC
606 GTTAAC
MfeI 1046 CAATTG C^AATTG
1088 CAATTG
MunI 1046 CAATTG C^AATTG
1088 CAATTG
PleI 307 GACTC GAGTC(4/5)
490 GAGTC
PvuII 1199 CAGCTG CAG^CTG
1669 CAGCTG
RsaI 178 GTAC GT^AC
1968 GTAC
ScrFI 785 CCAGG CC^[ACGT]GG
974 CCGGG
SfcI 755 CTACAG C^T[GA][CT]AG
797 CTACAG
TaqI 792 TCGA T^CGA
1134 TCGA
TthHB8I 792 TCGA T^CGA
1134 TCGA
XbaI 1449 TCTAGA T^CTAGA
1873 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BfaI BglI BsmAI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MboI MboII MflI MnlI MseI
MspA1I MspI MwoI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I SfaNI
SmlI TfiI Tru9I TseI Tsp509I
TspRI XhoII