Restriction map for
pF1KB7600
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BanI
BbeI BbsI BbvCI BcgI BciVI
BfrI BglII BlnI BpuAI BsaBI
BsaI BseAI BsiEI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI KasI KpnI Ksp632I
MamI MflI MluI MroI MseI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 664 GACGTC GACGT^C
AccI 565 GTCGAC GT^[AC][GT]AC
AgeI 54 ACCGGT A^CCGGT
AlwI 240 GATCC GGATC(4/5)
AspHI 754 GAGCAC G[AT]GC[AT]^C
AspI 492 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1242 CACGTG CAC^GTG
BfaI 410 CTAG C^TAG
BsaAI 1242 CACGTG [CT]AC^GT[GA]
BsaWI 54 ACCGGT [AT]^CCGG[AT]
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
BsiWI 708 CGTACG C^GTACG
BsrBI 477 GAGCGG CCGCTC(-3/-3)
BsrDI 996 CATTGC GCAATG(2/0)
BssSI 1249 CTCGTG CACGAG(-5/-1)
BstAPI 130 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1207 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 565 GTCGAC GT[CT]^[GA]AC
HindII 565 GTCGAC GT[CT]^[GA]AC
HindIII 769 AAGCTT A^AGCTT
KspI 462 CCGCGG CCGC^GG
MaeI 410 CTAG C^TAG
MfeI 436 CAATTG C^AATTG
MunI 436 CAATTG C^AATTG
NspI 92 GCATGC [GA]CATG^[CT]
PflFI 492 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 54 ACCGGT A^CCGGT
PleI 967 GAGTC GAGTC(4/5)
PmaCI 1242 CACGTG CAC^GTG
PmlI 1242 CACGTG CAC^GTG
SacII 462 CCGCGG CCGC^GG
SalI 565 GTCGAC G^TCGAC
SexAI 1070 ACCAGGT A^CC[AT]GGT
SmaI 971 CCCGGG CCC^GGG
SphI 92 GCATGC GCATG^C
TfiI 1271 GAATC G^A[AT]TC
Tsp509I 437 AATT ^AATT
Tth111I 492 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 971 CCCGGG C^CCGGG
XmaI 971 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 514 GGCGTC G[GA]^CG[CT]C
664 GACGTC
AhdI 118 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
664 GACGTCGCGTC
ApaI 392 GGGCCC GGGCC^C
930 GGGCCC
AspEI 118 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
664 GACGTCGCGTC
AvaI 934 CCCGAG C^[CT]CG[GA]G
971 CCCGGG
BalI 86 TGGCCA TGG^CCA
428 TGGCCA
BclI 101 TGATCA T^GATCA
858 TGATCA
BlpI 232 GCTCAGC GC^T[ACGT]AGC
903 GCTCAGC
Bpu1102I 232 GCTCAGC GC^T[ACGT]AGC
903 GCTCAGC
BsaHI 514 GGCGTC G[GA]^CG[CT]C
664 GACGTC
BsmI 1040 GCATTC GAATGC(1/-1)
1154 GCATTC
BsoBI 934 CCCGAG C^[CT]CG[GA]G
971 CCCGGG
BsrFI 54 ACCGGT [GA]^CCGG[CT]
508 ACCGGC
BsrI 630 ACTGG ACTGG(1/-1)
68 CCAGT
BstXI 68 CCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCACATCCCTGG
CelII 232 GCTCAGC GC^T[ACGT]AGC
903 GCTCAGC
Cfr10I 54 ACCGGT [GA]^CCGG[CT]
508 ACCGGC
DsaI 338 CCACGG C^C[GA][CT]GG
462 CCGCGG
EaeI 86 TGGCCA [CT]^GGCC[GA]
428 TGGCCA
Eam1105I 118 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
664 GACGTCGCGTC
FbaI 101 TGATCA T^GATCA
858 TGATCA
Hin1I 514 GGCGTC G[GA]^CG[CT]C
664 GACGTC
HinfI 967 GAGTC G^A[ACGT]TC
1271 GAATC
HphI 39 GGTGA GGTGA(8/7)
980 TCACC
MboII 49 GAAGA GAAGA(8/7)
1296 GAAGA
MluNI 86 TGGCCA TGG^CCA
428 TGGCCA
MscI 86 TGGCCA TGG^CCA
428 TGGCCA
PflMI 396 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCACATGGTGG
PvuII 811 CAGCTG CAG^CTG
906 CAGCTG
Van91I 396 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCACATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI