Restriction map for
pF1KB3384
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmFI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI MfeI MroI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SexAI SfoI SgrAI
SnaBI SpeI SphI SspBI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AocI 1264 CCTGAGG CC^T[ACGT]AGG
BanII 3 GAGCTC G[GA]GC[CT]^C
BcgI 521 GCATGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 509 GGATAC GTATCC(6/5)
BfaI 1849 CTAG C^TAG
BglI 22 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1158 TACGTG [CT]AC^GT[GA]
BsaBI 1126 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 254 GAGACC GGTCTC(1/5)
BsmBI 1814 GAGACG CGTCTC(1/5)
BspLU11I 513 ACATGT A^CATGT
BspMI 757 GCAGGT ACCTGC(4/8)
BsrDI 355 GCAATG GCAATG(2/0)
BsrFI 269 GCCGGT [GA]^CCGG[CT]
BstAPI 1039 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 529 TTCGAA TT^CGAA
Bsu36I 1264 CCTGAGG CC^T[ACGT]AGG
CfoI 888 GCGC GCG^C
Cfr10I 269 GCCGGT [GA]^CCGG[CT]
DrdI 460 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1585 CCGCGG C^C[GA][CT]GG
Eco81I 1264 CCTGAGG CC^T[ACGT]AGG
EcoNI 769 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 233 GATATC GAT^ATC
EcoT22I 710 ATGCAT ATGCA^T
FauI 1708 GCGGG CCCGC(4/6)
HaeII 887 AGCGCC [GA]GCGC^[CT]
HhaI 888 GCGC GCG^C
HinP1I 888 GCGC G^CGC
HindIII 289 AAGCTT A^AGCTT
KspI 1585 CCGCGG CCGC^GG
MaeI 1849 CTAG C^TAG
MamI 1126 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1672 ACGCGT A^CGCGT
MseI 1699 TTAA T^TAA
MslI 1990 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 713 CATATG CA^TATG
NsiI 710 ATGCAT ATGCA^T
NspI 513 ACATGT [GA]CATG^[CT]
NspV 529 TTCGAA TT^CGAA
PleI 1372 GAGTC GAGTC(4/5)
SacI 3 GAGCTC GAGCT^C
SacII 1585 CCGCGG CCGC^GG
SbfI 891 CCTGCAGG CCTGCA^GG
SfiI 21 GGCCATTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 529 TTCGAA TT^CGAA
SmaI 1550 CCCGGG CCC^GGG
SmlI 553 CTCAAG C^T[CT][GA]AG
Sse8387I 891 CCTGCAGG CCTGCA^GG
SspI 1886 AATATT AAT^ATT
StuI 582 AGGCCT AGG^CCT
Tru9I 1699 TTAA T^TAA
XcmI 1188 CCATATTGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1550 CCCGGG C^CCGGG
XmaI 1550 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 1586 CGCG CG^CG
1673 CGCG
AflIII 513 ACATGT A^C[GA][CT]GT
1672 ACGCGT
BamHI 1411 GGATCC G^GATCC
1581 GGATCC
BsgI 959 CTGCAC GTGCAG(16/14)
2000 CTGCAC
BsmI 1590 GAATGC GAATGC(1/-1)
2068 GAATGC
BsrBI 1683 CCGCTC CCGCTC(-3/-3)
1706 GAGCGG
BstUI 1586 CGCG CG^CG
1673 CGCG
DraII 206 AGGACCC [GA]G^G[ACGT]CC[CT]
620 AGGTCCT
DraIII 741 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1740 CACGGCGTG
EcoO109I 206 AGGACCC [GA]G^G[ACGT]CC[CT]
620 AGGTCCT
EcoRI 1897 GAATTC G^AATTC
2091 GAATTC
HgaI 1671 GACGC GACGC(5/10)
1674 GCGTC
MaeII 594 ACGT A^CGT
1159 ACGT
MaeIII 446 GTGAC ^GT[ACGT]AC
1374 GTCAC
MvnI 1586 CGCG CG^CG
1673 CGCG
PflMI 740 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1789 CCACATTCTGG
PpuMI 206 AGGACCC [GA]G^G[AT]CC[CT]
620 AGGTCCT
PstI 782 CTGCAG CTGCA^G
892 CTGCAG
ScaI 534 AGTACT AGT^ACT
1522 AGTACT
TfiI 1168 GAATC G^A[AT]TC
1572 GAATC
Tsp45I 446 GTGAC ^GT[GC]AC
1374 GTCAC
Van91I 740 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1789 CCACATTCTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BalI BbsI BbvCI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XhoII XmnI