Restriction map for
pF1KB7752
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRV FbaI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwNI 282 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BpmI 1330 CTGGAG CTGGAG(16/14)
BsaWI 1428 ACCGGA [AT]^CCGG[AT]
BsgI 1120 GTGCAG GTGCAG(16/14)
BsiEI 1162 CGGCCG CG[GA][CT]^CG
BsmBI 1324 CGTCTC CGTCTC(1/5)
BsmI 1211 GAATGC GAATGC(1/-1)
Bsp1407I 1136 TGTACA T^GTACA
BsrGI 1136 TGTACA T^GTACA
BstEII 59 GGTCACC G^GT[ACGT]ACC
BstPI 59 GGTCACC G^GT[ACGT]ACC
BstXI 362 CCATGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 495 GGATCT [GA]^GATC[CT]
DraII 1442 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 1162 CGGCCG C^GGCCG
EclXI 1162 CGGCCG C^GGCCG
Eco52I 1162 CGGCCG C^GGCCG
EcoNI 747 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1442 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 59 GGTCACC G^GT[ACGT]ACC
EcoRI 1352 GAATTC G^AATTC
EcoT22I 1213 ATGCAT ATGCA^T
FseI 1159 GGCCGGCC GGCCGG^CC
MaeII 1055 ACGT A^CGT
MflI 495 GGATCT [GA]^GATC[CT]
MseI 926 TTAA T^TAA
NaeI 1160 GCCGGC GCC^GGC
NcoI 362 CCATGG C^CATGG
NgoMIV 1160 GCCGGC G^CCGGC
NsiI 1213 ATGCAT ATGCA^T
PpuMI 1442 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1410 GAGCTC GAGCT^C
SapI 1308 GAAGAGC GCTCTTC(1/4)
SfaNI 344 GCATC GCATC(5/9)
SspBI 1136 TGTACA T^GTACA
TfiI 1131 GATTC G^A[AT]TC
Tru9I 926 TTAA T^TAA
XbaI 375 TCTAGA T^CTAGA
XhoII 495 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 568 AAATTC [GA]^AATT[CT]
1352 GAATTC
AcyI 889 GACGCC G[GA]^CG[CT]C
1180 GGCGTC
AfaI 1032 GTAC GT^AC
1137 GTAC
ApoI 568 AAATTC [GA]^AATT[CT]
1352 GAATTC
AspHI 1048 GAGCAC G[AT]GC[AT]^C
1410 GAGCTC
BbsI 733 GAAGAC GAAGAC(2/6)
1381 GAAGAC
BfaI 131 CTAG C^TAG
376 CTAG
BglI 511 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1160 GCCGGCCGGGC
BpuAI 733 GAAGAC GAAGAC(2/6)
1381 GAAGAC
BsaHI 889 GACGCC G[GA]^CG[CT]C
1180 GGCGTC
BsiHKAI 1048 GAGCAC G[AT]GC[AT]^C
1410 GAGCTC
BsmFI 170 GGGAC GGGAC(10/14)
777 GTCCC
BspMI 1034 ACCTGC ACCTGC(4/8)
1233 GCAGGT
BsrDI 507 CATTGC GCAATG(2/0)
1454 GCAATG
BsrFI 1160 GCCGGC [GA]^CCGG[CT]
1284 GCCGGT
Cfr10I 1160 GCCGGC [GA]^CCGG[CT]
1284 GCCGGT
DsaI 350 CCGTGG C^C[GA][CT]GG
362 CCATGG
EcoT14I 362 CCATGG C^C[AT][AT]GG
1278 CCAAGG
Hin1I 889 GACGCC G[GA]^CG[CT]C
1180 GGCGTC
HincII 975 GTCAAC GT[CT]^[GA]AC
1057 GTCAAC
HindII 975 GTCAAC GT[CT]^[GA]AC
1057 GTCAAC
MaeI 131 CTAG C^TAG
376 CTAG
NlaIV 1227 GGGTCC GG[ACGT]^[ACGT]CC
1425 GGAACC
NspI 113 GCATGT [GA]CATG^[CT]
1215 GCATGT
PstI 460 CTGCAG CTGCA^G
955 CTGCAG
PvuII 282 CAGCTG CAG^CTG
322 CAGCTG
RsaI 1032 GTAC GT^AC
1137 GTAC
SfcI 460 CTGCAG C^T[GA][CT]AG
955 CTGCAG
SmlI 859 CTCAAG C^T[CT][GA]AG
1246 CTTGAG
StyI 362 CCATGG C^C[AT][AT]GG
1278 CCAAGG
Tsp509I 569 AATT ^AATT
1353 AATT
XcmI 674 CCATCACCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
893 CCATCTTTGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
PleI Sau3AI Sau96I ScrFI TaqI
TseI Tsp45I TspRI TthHB8I