Restriction map for
pF1KE0297
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AcsI AhdI ApoI AscI
AseI AsnI AspEI AspI AvrII
BbrPI BclI BlnI BsmI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DraI DrdI
Eam1105I EarI EcoRI EcoRV EcoT22I
FbaI HindIII HpaI Ksp632I MfeI
MunI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 6 GACGTC GACGT^C
Acc65I 215 GGTACC G^GTACC
AccI 3460 GTAGAC GT^[AC][GT]AC
AccIII 1742 TCCGGA T^CCGGA
AflII 472 CTTAAG C^TTAAG
AgeI 2180 ACCGGT A^CCGGT
Aor51HI 2954 AGCGCT AGC^GCT
Asp700I 1443 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 215 GGTACC G^GTACC
AviII 2156 TGCGCA TGC^GCA
BamHI 53 GGATCC G^GATCC
BciVI 3255 GTATCC GTATCC(6/5)
BfrI 472 CTTAAG C^TTAAG
BglII 1257 AGATCT A^GATCT
BsaBI 4552 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1742 TCCGGA T^CCGGA
BsiWI 359 CGTACG C^GTACG
BsmBI 4 GAGACG CGTCTC(1/5)
BspEI 1742 TCCGGA T^CCGGA
BspLU11I 4355 ACATGT A^CATGT
BsrDI 1799 GCAATG GCAATG(2/0)
BstEII 2385 GGTGACC G^GT[ACGT]ACC
BstPI 2385 GGTGACC G^GT[ACGT]ACC
CpoI 1643 CGGACCG CG^G[AT]CCG
Eco47III 2954 AGCGCT AGC^GCT
EcoO65I 2385 GGTGACC G^GT[ACGT]ACC
FseI 1154 GGCCGGCC GGCCGG^CC
FspI 2156 TGCGCA TGC^GCA
KpnI 215 GGTACC GGTAC^C
MamI 4552 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2582 ACGCGT A^CGCGT
MroI 1742 TCCGGA T^CCGGA
MseI 473 TTAA T^TAA
NdeI 1595 CATATG CA^TATG
NheI 3577 GCTAGC G^CTAGC
PinAI 2180 ACCGGT A^CCGGT
RsrII 1643 CGGACCG CG^G[AT]CCG
SgrAI 2645 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4328 GCATGC GCATG^C
Tru9I 473 TTAA T^TAA
Tsp509I 3916 AATT ^AATT
XmnI 1443 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AocI 1141 CCTGAGG CC^T[ACGT]AGG
3499 CCTCAGG
BbsI 1324 GAAGAC GAAGAC(2/6)
1560 GAAGAC
BcgI 188 CGATTGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1656 GCACCAGAATCG
BpuAI 1324 GAAGAC GAAGAC(2/6)
1560 GAAGAC
BsaAI 361 TACGTG [CT]AC^GT[GA]
4157 TACGTG
BsaI 1306 GAGACC GGTCTC(1/5)
2075 GAGACC
BsaWI 1742 TCCGGA [AT]^CCGG[AT]
2180 ACCGGT
Bsu36I 1141 CCTGAGG CC^T[ACGT]AGG
3499 CCTCAGG
DraIII 241 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2971 CACAACGTG
Eco81I 1141 CCTGAGG CC^T[ACGT]AGG
3499 CCTCAGG
HincII 1096 GTCAAC GT[CT]^[GA]AC
4477 GTCAAC
HindII 1096 GTCAAC GT[CT]^[GA]AC
4477 GTCAAC
NcoI 983 CCATGG C^CATGG
4205 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI