Restriction map for
pF1KE1055
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# NO CUT ENZYMES ( 54 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
AscI AspEI AspI AviII AvrII
BfrI BlnI BsaBI BsiWI Bsp1407I
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI Eam1105I EcoRV FspI
HincII HindII HpaI MamI MluI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacI SalI ScaI SexAI
SfiI SfuI SgrAI SpeI SspBI
SspI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 3962 GGTACC G^GTACC
AlwNI 3081 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2591 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4644 AGCGCT AGC^GCT
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
Asp718I 3962 GGTACC G^GTACC
BamHI 1184 GGATCC G^GATCC
BbrPI 286 CACGTG CAC^GTG
BcgI 2825 CGACGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 501 GTATCC GTATCC(6/5)
BclI 254 TGATCA T^GATCA
BglII 1702 AGATCT A^GATCT
BspDI 2230 ATCGAT AT^CGAT
BspHI 423 TCATGA T^CATGA
BspLU11I 1878 ACATGT A^CATGT
BsrDI 464 CATTGC GCAATG(2/0)
BstEII 1279 GGTGACC G^GT[ACGT]ACC
BstPI 1279 GGTGACC G^GT[ACGT]ACC
Bsu36I 2591 CCTCAGG CC^T[ACGT]AGG
ClaI 2230 ATCGAT AT^CGAT
DraI 3297 TTTAAA TTT^AAA
DraIII 2998 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4644 AGCGCT AGC^GCT
Eco81I 2591 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1279 GGTGACC G^GT[ACGT]ACC
EcoRI 51 GAATTC G^AATTC
EcoT22I 1075 ATGCAT ATGCA^T
FbaI 254 TGATCA T^GATCA
FseI 2650 GGCCGGCC GGCCGG^CC
HindIII 4125 AAGCTT A^AGCTT
KpnI 3962 GGTACC GGTAC^C
MfeI 407 CAATTG C^AATTG
MunI 407 CAATTG C^AATTG
NsiI 1075 ATGCAT ATGCA^T
PmaCI 286 CACGTG CAC^GTG
PmlI 286 CACGTG CAC^GTG
PshAI 4231 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 589 ATTAAT AT^TAAT
RcaI 423 TCATGA T^CATGA
SbfI 4244 CCTGCAGG CCTGCA^GG
SnaBI 498 TACGTA TAC^GTA
Sse8387I 4244 CCTGCAGG CCTGCA^GG
SwaI 3296 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 933 GACGTC GACGT^C
2509 GACGTC
AflIII 285 ACACGT A^C[GA][CT]GT
1878 ACATGT
Alw44I 1492 GTGCAC G^TGCAC
3797 GTGCAC
ApaLI 1492 GTGCAC G^TGCAC
3797 GTGCAC
BbeI 2966 GGCGCC GGCGC^C
4178 GGCGCC
BsaI 2216 GAGACC GGTCTC(1/5)
2409 GGTCTC
BsmBI 935 CGTCTC CGTCTC(1/5)
1062 CGTCTC
BsmI 2062 GAATGC GAATGC(1/-1)
1800 GCATTC
BstAPI 1485 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3681 GCACTTGATGC
EagI 1945 CGGCCG C^GGCCG
2653 CGGCCG
EclXI 1945 CGGCCG C^GGCCG
2653 CGGCCG
Eco52I 1945 CGGCCG C^GGCCG
2653 CGGCCG
KasI 2966 GGCGCC G^GCGCC
4178 GGCGCC
MseI 590 TTAA T^TAA
3298 TTAA
NaeI 781 GCCGGC GCC^GGC
2651 GCCGGC
NarI 2966 GGCGCC GG^CGCC
4178 GGCGCC
NgoMIV 781 GCCGGC G^CCGGC
2651 GCCGGC
SapI 3843 GAAGAGC GCTCTTC(1/4)
980 GCTCTTC
SfoI 2966 GGCGCC GGC^GCC
4178 GGCGCC
SphI 3932 GCATGC GCATG^C
3992 GCATGC
StuI 1171 AGGCCT AGG^CCT
1664 AGGCCT
Tru9I 590 TTAA T^TAA
3298 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoI XhoII
XmaCI XmaI XmnI