Restriction map for
pF1KB5980
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AhdI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvrII
BamHI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KspI MfeI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 233 GGTACC G^GTACC
AccII 1035 CGCG CG^CG
AflIII 218 ACGTGT A^C[GA][CT]GT
Alw44I 1246 GTGCAC G^TGCAC
AocI 1049 CCTCAGG CC^T[ACGT]AGG
Aor51HI 305 AGCGCT AGC^GCT
ApaI 76 GGGCCC GGGCC^C
ApaLI 1246 GTGCAC G^TGCAC
Asp718I 233 GGTACC G^GTACC
AviII 245 TGCGCA TGC^GCA
BbrPI 238 CACGTG CAC^GTG
BsaAI 238 CACGTG [CT]AC^GT[GA]
BsgI 586 CTGCAC GTGCAG(16/14)
BsiEI 1020 CGGCCG CG[GA][CT]^CG
BsmI 1395 GCATTC GAATGC(1/-1)
BstUI 1035 CGCG CG^CG
BstYI 452 GGATCT [GA]^GATC[CT]
Bsu36I 1049 CCTCAGG CC^T[ACGT]AGG
EagI 1020 CGGCCG C^GGCCG
EclXI 1020 CGGCCG C^GGCCG
Eco47III 305 AGCGCT AGC^GCT
Eco52I 1020 CGGCCG C^GGCCG
Eco81I 1049 CCTCAGG CC^T[ACGT]AGG
EcoT14I 881 CCATGG C^C[AT][AT]GG
FspI 245 TGCGCA TGC^GCA
HgaI 670 GACGC GACGC(5/10)
KpnI 233 GGTACC GGTAC^C
MflI 452 GGATCT [GA]^GATC[CT]
MslI 111 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1035 CGCG CG^CG
NcoI 881 CCATGG C^CATGG
PmaCI 238 CACGTG CAC^GTG
PmlI 238 CACGTG CAC^GTG
PvuII 356 CAGCTG CAG^CTG
SapI 564 GAAGAGC GCTCTTC(1/4)
SgrAI 113 CACCGGTG C[GA]^CCGG[CT]G
StuI 65 AGGCCT AGG^CCT
StyI 881 CCATGG C^C[AT][AT]GG
XhoII 452 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AgeI 114 ACCGGT A^CCGGT
778 ACCGGT
AlwI 452 GGATC GGATC(4/5)
521 GGATC
AspHI 1246 GTGCAC G[AT]GC[AT]^C
1341 GAGCAC
BalI 107 TGGCCA TGG^CCA
1367 TGGCCA
BbvCI 1029 CCTCAGC CCTCAGC(-5/-2)
901 GCTGAGG
BcgI 1145 GCACACCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1352 GCATGGCCTTCG
BsaBI 1206 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1212 GATCAAGATC
BsaI 421 GAGACC GGTCTC(1/5)
1120 GAGACC
BsiHKAI 1246 GTGCAC G[AT]GC[AT]^C
1341 GAGCAC
BsrBI 738 CCGCTC CCGCTC(-3/-3)
850 GAGCGG
BsrFI 114 ACCGGT [GA]^CCGG[CT]
778 ACCGGT
Cfr10I 114 ACCGGT [GA]^CCGG[CT]
778 ACCGGT
DsaI 701 CCACGG C^C[GA][CT]GG
881 CCATGG
EcoNI 1557 CCTGTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1593 CCTAGAGCAGG
MaeII 218 ACGT A^CGT
239 ACGT
MamI 1206 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1212 GATCAAGATC
MluNI 107 TGGCCA TGG^CCA
1367 TGGCCA
MscI 107 TGGCCA TGG^CCA
1367 TGGCCA
PflMI 272 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
701 CCACGGAGTGG
PinAI 114 ACCGGT A^CCGGT
778 ACCGGT
PpuMI 860 GGGACCT [GA]G^G[AT]CC[CT]
981 AGGTCCC
PshAI 423 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1408 GACTACAGTC
PstI 130 CTGCAG CTGCA^G
865 CTGCAG
SfaNI 126 GATGC GCATC(5/9)
569 GCATC
SmlI 915 CTTGAG C^T[CT][GA]AG
1543 CTCAAG
Van91I 272 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
701 CCACGGAGTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
SfoI SmaI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI