Restriction map for
pF1KB6278
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI BstBI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KspI MamI
MluI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1834 GGTACC G^GTACC
AccI 1598 GTATAC GT^[AC][GT]AC
AccII 2165 CGCG CG^CG
AclI 1392 AACGTT AA^CGTT
AcyI 1467 GACGCC G[GA]^CG[CT]C
AflII 1725 CTTAAG C^TTAAG
AlwNI 2318 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 257 GGGCCC GGGCC^C
Asp700I 653 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1834 GGTACC G^GTACC
AvaI 837 CTCGGG C^[CT]CG[GA]G
BanI 1834 GGTACC G^G[CT][GA]CC
BbsI 1899 GAAGAC GAAGAC(2/6)
BfrI 1725 CTTAAG C^TTAAG
BglII 1098 AGATCT A^GATCT
BpuAI 1899 GAAGAC GAAGAC(2/6)
BsaHI 1467 GACGCC G[GA]^CG[CT]C
BsgI 296 CTGCAC GTGCAG(16/14)
BsiEI 242 CGGCCG CG[GA][CT]^CG
BsoBI 837 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1182 TGTACA T^GTACA
BsrGI 1182 TGTACA T^GTACA
Bst1107I 1598 GTATAC GTA^TAC
BstAPI 1422 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2165 CGCG CG^CG
BstXI 316 CCAAACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1598 GTATAC GTA^TAC
CfoI 2164 GCGC GCG^C
EagI 242 CGGCCG C^GGCCG
EclXI 242 CGGCCG C^GGCCG
Eco52I 242 CGGCCG C^GGCCG
EcoRV 2278 GATATC GAT^ATC
HgaI 1467 GACGC GACGC(5/10)
HhaI 2164 GCGC GCG^C
Hin1I 1467 GACGCC G[GA]^CG[CT]C
HinP1I 2164 GCGC G^CGC
KpnI 1834 GGTACC GGTAC^C
MfeI 719 CAATTG C^AATTG
MslI 2023 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 719 CAATTG C^AATTG
MvnI 2165 CGCG CG^CG
NcoI 1263 CCATGG C^CATGG
NlaIII 1264 CATG CATG^
NotI 241 GCGGCCGC GC^GGCCGC
Psp1406I 1392 AACGTT AA^CGTT
PvuII 1324 CAGCTG CAG^CTG
SacI 1916 GAGCTC GAGCT^C
SapI 569 GCTCTTC GCTCTTC(1/4)
SmlI 1725 CTTAAG C^T[CT][GA]AG
SspBI 1182 TGTACA T^GTACA
XbaI 819 TCTAGA T^CTAGA
XcmI 528 CCAGTCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 653 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 868 ATTAAT AT^TAAT
958 ATTAAT
AsnI 868 ATTAAT AT^TAAT
958 ATTAAT
AspHI 311 GAGCAC G[AT]GC[AT]^C
1916 GAGCTC
AvaII 1410 GGTCC G^G[AT]CC
1755 GGACC
BanII 257 GGGCCC G[GA]GC[CT]^C
1916 GAGCTC
BcnI 220 CCGGG CC^[GC]GG
254 CCGGG
BpmI 1526 CTCCAG CTGGAG(16/14)
1937 CTGGAG
BsiHKAI 311 GAGCAC G[AT]GC[AT]^C
1916 GAGCTC
BsmI 1620 GAATGC GAATGC(1/-1)
1932 GCATTC
BstYI 524 AGATCC [GA]^GATC[CT]
1098 AGATCT
DraII 256 GGGGCCC [GA]G^G[ACGT]CC[CT]
257 GGGCCCC
DsaI 87 CCACGG C^C[GA][CT]GG
1263 CCATGG
EcoO109I 256 GGGGCCC [GA]G^G[ACGT]CC[CT]
257 GGGCCCC
EcoT14I 44 CCTTGG C^C[AT][AT]GG
1263 CCATGG
FauI 262 CCCGC CCCGC(4/6)
942 GCGGG
MflI 524 AGATCC [GA]^GATC[CT]
1098 AGATCT
MspA1I 143 CAGCGG C[AC]G^C[GT]G
1324 CAGCTG
NciI 220 CCGGG CC^[GC]GG
254 CCGGG
PflMI 685 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1876 CCAGGATGTGG
PleI 833 GACTC GAGTC(4/5)
1204 GAGTC
PshBI 868 ATTAAT AT^TAAT
958 ATTAAT
SfaNI 1188 GCATC GCATC(5/9)
2149 GCATC
StyI 44 CCTTGG C^C[AT][AT]GG
1263 CCATGG
TaqI 815 TCGA T^CGA
1989 TCGA
TthHB8I 815 TCGA T^CGA
1989 TCGA
Van91I 685 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1876 CCAGGATGTGG
XhoII 524 AGATCC [GA]^GATC[CT]
1098 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI