Restriction map for
pF1KB9876
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspI AvaI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 567 GGTACC G^GTACC
AflIII 254 ACGTGT A^C[GA][CT]GT
ApaI 1081 GGGCCC GGGCC^C
Asp718I 567 GGTACC G^GTACC
BanI 567 GGTACC G^G[CT][GA]CC
BbrPI 253 CACGTG CAC^GTG
BbvCI 900 CCTCAGC CCTCAGC(-5/-2)
BglII 90 AGATCT A^GATCT
BpmI 461 CTGGAG CTGGAG(16/14)
BsaAI 253 CACGTG [CT]AC^GT[GA]
BsaI 456 GGTCTC GGTCTC(1/5)
BsiEI 325 CGATCG CG[GA][CT]^CG
BsmAI 457 GTCTC GTCTC(1/5)
BspMI 1003 ACCTGC ACCTGC(4/8)
BsrDI 751 GCAATG GCAATG(2/0)
BssSI 1460 CTCGTG CACGAG(-5/-1)
DsaI 1076 CCATGG C^C[GA][CT]GG
EcoRV 7 GATATC GAT^ATC
KpnI 567 GGTACC GGTAC^C
NaeI 1367 GCCGGC GCC^GGC
NcoI 1076 CCATGG C^CATGG
NgoMIV 1367 GCCGGC G^CCGGC
NspI 114 GCATGT [GA]CATG^[CT]
PmaCI 253 CACGTG CAC^GTG
PmlI 253 CACGTG CAC^GTG
PvuI 325 CGATCG CGAT^CG
SacI 381 GAGCTC GAGCT^C
SexAI 366 ACCTGGT A^CC[AT]GGT
SspI 925 AATATT AAT^ATT
TaqI 328 TCGA T^CGA
TthHB8I 328 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 381 GAGCTC G[AT]GC[AT]^C
1402 GAGCAC
BanII 381 GAGCTC G[GA]GC[CT]^C
1081 GGGCCC
BsaBI 79 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
85 GATCCAGATC
BseRI 70 GAGGAG GAGGAG(10/8)
464 GAGGAG
BsiHKAI 381 GAGCTC G[AT]GC[AT]^C
1402 GAGCAC
BsmFI 430 GGGAC GGGAC(10/14)
586 GTCCC
Bsp1407I 117 TGTACA T^GTACA
1065 TGTACA
BsrGI 117 TGTACA T^GTACA
1065 TGTACA
BsrI 169 CCAGT ACTGG(1/-1)
404 CCAGT
DraI 892 TTTAAA TTT^AAA
1429 TTTAAA
MamI 79 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
85 GATCCAGATC
MslI 121 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1071 CATCGCCATG
MspA1I 903 CAGCTG C[AC]G^C[GT]G
984 CAGCTG
PpuMI 1130 AGGTCCT [GA]G^G[AT]CC[CT]
1346 GGGTCCT
PvuII 903 CAGCTG CAG^CTG
984 CAGCTG
SfcI 1045 CTACAG C^T[GA][CT]AG
1372 CTACAG
SspBI 117 TGTACA T^GTACA
1065 TGTACA
XcmI 654 CCATGTTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1155 CCATCATAAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AhdI AluI AlwI
ApoI AspEI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrFI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII Eam1105I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII