##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI           21  CCGC                 CCGC(-3/-1)
AcsI          229  GAATTC               [GA]^AATT[CT]
AfaI          299  GTAC                 GT^AC
AflIII        206  ACATGT               A^C[GA][CT]GT
AocI          142  CCTGAGG              CC^T[ACGT]AGG
ApoI          229  GAATTC               [GA]^AATT[CT]
AseI          618  ATTAAT               AT^TAAT
AsnI          618  ATTAAT               AT^TAAT
AvaII         268  GGTCC                G^G[AT]CC
BanI          603  GGCACC               G^G[CT][GA]CC
BbvCI          12  CCTCAGC              CCTCAGC(-5/-2)
BfaI          106  CTAG                 C^TAG
BseRI         523  GAGGAG               GAGGAG(10/8)
BsmAI         123  GAGAC                GTCTC(1/5)
BsmFI         412  GTCCC                GGGAC(10/14)
BspLU11I      206  ACATGT               A^CATGT
BspMI         265  GCAGGT               ACCTGC(4/8)
BsrBI          21  CCGCTC               CCGCTC(-3/-3)
Bsu36I        142  CCTGAGG              CC^T[ACGT]AGG
Eco81I        142  CCTGAGG              CC^T[ACGT]AGG
EcoRI         229  GAATTC               G^AATTC
HaeIII        528  GGCC                 GG^CC
HgaI          459  GCGTC                GACGC(5/10)
HphI          489  TCACC                GGTGA(8/7)
MaeI          106  CTAG                 C^TAG
MslI           70  CATCAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        514  CAGCTG               C[AC]G^C[GT]G
NlaIV         603  GGCACC               GG[ACGT]^[ACGT]CC
PshBI         618  ATTAAT               AT^TAAT
PstI          263  CTGCAG               CTGCA^G
PvuII         514  CAGCTG               CAG^CTG
RsaI          299  GTAC                 GT^AC
SapI           23  GCTCTTC              GCTCTTC(1/4)
SfaNI         510  GATGC                GCATC(5/9)
SfcI          263  CTGCAG               C^T[GA][CT]AG
SspI          304  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BsgI          234  CTGCAC               GTGCAG(16/14)
              329  CTGCAC              
BsrI          287  ACTGG                ACTGG(1/-1)
              253  CCAGT               
BstYI          64  GGATCT               [GA]^GATC[CT]
              153  GGATCT              
Cac8I         244  GCAAGC               GC[ACGT]^[ACGT]GC
              600  GCTGGC              
Cfr13I        268  GGTCC                G^G[ACGT]CC
              528  GGCCC               
EarI           24  CTCTTC               CTCTTC(1/4)
              608  CTCTTC              
HinfI         117  GAATC                G^A[ACGT]TC
              502  GATTC               
Ksp632I        24  CTCTTC               CTCTTC(1/4)
              608  CTCTTC              
MflI           64  GGATCT               [GA]^GATC[CT]
              153  GGATCT              
NspI           90  ACATGC               [GA]CATG^[CT]
              206  ACATGT              
Sau96I        268  GGTCC                G^G[ACGT]CC
              528  GGCCC               
TfiI          117  GAATC                G^A[AT]TC
              502  GATTC               
Tsp509I       230  AATT                 ^AATT
              617  AATT                
XhoII          64  GGATCT               [GA]^GATC[CT]
              153  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        AlwI        BbvI        DdeI        DpnII       
Fnu4HI      ItaI        MboI        MboII       MnlI        
MseI        NdeII       NlaIII      Sau3AI      Tru9I       
TseI        TspRI