Restriction map for
pF1KB9453
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfaI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsgI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FbaI FseI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MunI
MvnI NarI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1077 GTCTAC GT^[AC][GT]AC
AccIII 360 TCCGGA T^CCGGA
AflII 783 CTTAAG C^TTAAG
AocI 226 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1603 AGCGCT AGC^GCT
AspHI 798 GAGCTC G[AT]GC[AT]^C
AviII 194 TGCGCA TGC^GCA
BbrPI 401 CACGTG CAC^GTG
BfrI 783 CTTAAG C^TTAAG
BsaAI 401 CACGTG [CT]AC^GT[GA]
BsaBI 522 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 360 TCCGGA T^CCGGA
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiHKAI 798 GAGCTC G[AT]GC[AT]^C
BsmBI 1067 CGTCTC CGTCTC(1/5)
BsmI 104 GAATGC GAATGC(1/-1)
BspEI 360 TCCGGA T^CCGGA
BspHI 419 TCATGA T^CATGA
BspMI 375 ACCTGC ACCTGC(4/8)
BsrBI 322 GAGCGG CCGCTC(-3/-3)
BsrFI 1777 GCCGGC [GA]^CCGG[CT]
BssSI 1599 CACGAG CACGAG(-5/-1)
BstYI 779 GGATCT [GA]^GATC[CT]
Bsu36I 226 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1777 GCCGGC [GA]^CCGG[CT]
DraIII 828 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1603 AGCGCT AGC^GCT
Eco81I 226 CCTGAGG CC^T[ACGT]AGG
EcoNI 675 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1107 GAATTC G^AATTC
EcoT22I 1144 ATGCAT ATGCA^T
FspI 194 TGCGCA TGC^GCA
HaeII 1603 AGCGCT [GA]GCGC^[CT]
MamI 522 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 779 GGATCT [GA]^GATC[CT]
MroI 360 TCCGGA T^CCGGA
MspA1I 60 CCGCTG C[AC]G^C[GT]G
NaeI 1777 GCCGGC GCC^GGC
NgoMIV 1777 GCCGGC G^CCGGC
NsiI 1144 ATGCAT ATGCA^T
PaeR7I 134 CTCGAG C^TCGAG
PflMI 1762 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 401 CACGTG CAC^GTG
PmlI 401 CACGTG CAC^GTG
RcaI 419 TCATGA T^CATGA
SacI 798 GAGCTC GAGCT^C
SmaI 1711 CCCGGG CCC^GGG
SspI 935 AATATT AAT^ATT
StuI 886 AGGCCT AGG^CCT
Tsp45I 1837 GTCAC ^GT[GC]AC
Van91I 1762 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 134 CTCGAG C^TCGAG
XhoII 779 GGATCT [GA]^GATC[CT]
XmaCI 1711 CCCGGG C^CCGGG
XmaI 1711 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 877 AAATTC [GA]^AATT[CT]
1107 GAATTC
AflIII 400 ACACGT A^C[GA][CT]GT
402 ACGTGT
AlwNI 977 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1345 CAGTACCTG
ApoI 877 AAATTC [GA]^AATT[CT]
1107 GAATTC
BanI 1319 GGCACC G^G[CT][GA]CC
1698 GGTGCC
BsaI 203 GAGACC GGTCTC(1/5)
805 GAGACC
BsaWI 7 ACCGGA [AT]^CCGG[AT]
360 TCCGGA
BsrDI 861 CATTGC GCAATG(2/0)
512 GCAATG
MaeII 402 ACGT A^CGT
641 ACGT
MaeIII 619 GTTAC ^GT[ACGT]AC
1837 GTCAC
NspI 1142 GCATGC [GA]CATG^[CT]
1865 GCATGC
PpuMI 235 AGGTCCC [GA]G^G[AT]CC[CT]
754 GGGACCC
PstI 166 CTGCAG CTGCA^G
377 CTGCAG
SmlI 134 CTCGAG C^T[CT][GA]AG
783 CTTAAG
SphI 1142 GCATGC GCATG^C
1865 GCATGC
TaqI 135 TCGA T^CGA
689 TCGA
TfiI 96 GATTC G^A[AT]TC
423 GATTC
Tsp509I 878 AATT ^AATT
1108 AATT
TthHB8I 135 TCGA T^CGA
689 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
Tru9I TseI TspRI XcmI